Search results for the GEO ID: GSE36392  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM892312 | GPL1261 |
|
T2M cells_replicate 1
|
T2M
|
cell surface markers: IL-4/GFP+ IL-5rα- F4/80+ MHCIIlo CD11b+ Gr-1mid
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: T2M
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892312
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892312/suppl/GSM892312_Lukacs_002_B_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892313 | GPL1261 |
|
T2M cells_replicate 2
|
T2M
|
cell surface markers: IL-4/GFP+ IL-5rα- F4/80+ MHCIIlo CD11b+ Gr-1mid
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: T2M
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892313
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892313/suppl/GSM892313_Lukacs_003_C_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892314 | GPL1261 |
|
T2M cells_replicate 3
|
T2M
|
cell surface markers: IL-4/GFP+ IL-5rα- F4/80+ MHCIIlo CD11b+ Gr-1mid
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: T2M
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892314
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892314/suppl/GSM892314_Lukacs_004_D_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892315 | GPL1261 |
|
Eosinophils_replicate 1
|
Eosinophils
|
cell surface markers: IL-4/GFP- IL-5rα+ F4/80- MHCIIlo CD11b+ Gr-1hi
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: Eosinophils
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892315
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892315/suppl/GSM892315_Lukacs_005_E_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892316 | GPL1261 |
|
Eosinophils_replicate 2
|
Eosinophils
|
cell surface markers: IL-4/GFP- IL-5rα+ F4/80- MHCIIlo CD11b+ Gr-1hi
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: Eosinophils
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892316
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892316/suppl/GSM892316_Lukacs_006_F_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892317 | GPL1261 |
|
Macrophages_replicate 1
|
Macrophages
|
cell surface markers: IL-4/GFP- IL-5rα- F4/80+ MHCII+ CD11b+ Gr-1-
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: Macrophages
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892317
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892317/suppl/GSM892317_Lukacs_007_G_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892318 | GPL1261 |
|
Macrophages_replicate 2
|
Macrophages
|
cell surface markers: IL-4/GFP- IL-5rα- F4/80+ MHCII+ CD11b+ Gr-1-
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: Macrophages
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892318
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892318/suppl/GSM892318_Lukacs_008_H_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892319 | GPL1261 |
|
Neutrophils_replicate 1
|
Neutrophils
|
cell surface markers: IL-4/GFP- IL-5rα- F4/80- MHCIIlo CD11b+ Gr-1+
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: Neutrophils
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892319
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892319/suppl/GSM892319_Lukacs_009_I_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
| | |
|
GSM892320 | GPL1261 |
|
Neutrophils_replicate 2
|
Neutrophils
|
cell surface markers: IL-4/GFP- IL-5rα- F4/80- MHCIIlo CD11b+ Gr-1+
background strain: BALB/c
genotype/variation: 4get
tissue type: lung
cell type: Neutrophils
|
Lung, IL-25 treated
|
| Sample_geo_accession | GSM892320
| | Sample_status | Public on Mar 10 2012
| | Sample_submission_date | Mar 09 2012
| | Sample_last_update_date | Mar 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lung tissue was processed via enzymatic digestion with 1 mg/ml collagenase A (Roche) in RPMI 1640 (Invitrogen) with 5% FCS (Atlas Biologicals) and 2 U DNase (Sigma-Aldrich) at 37°C for 1 hour, and cells dispersed by passing digest through an 18-gauge cannula. Following RBC lysis,
| | Sample_treatment_protocol_ch1 | MACS columns (Miltenyl) were used to enrich for CD11b+ myleoid populations. Cells were pooled from 4 donor mice and stained for flow cytometry using standard techniques. Cells surface markers used to discriminate between inflammatory subsets are listed in the sample description column.
| | Sample_growth_protocol_ch1 | 6–8 week old female 4get mice received daily intra-tracheal injections (0.5 μg recombinant IL-25 (R&D) in 50 μL PBS) for 4 days to induce antigen-independent type 2 inflammation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 400,000 cells were collected by FACS for each sample and lysed directly upon sorting in RLT lysis buffer. Total RNA was isolated with Qiagen kits according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 50 ng total RNA (ovation pico WTA system V2. PN3302)
| | Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| | Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Bryan,C,Petersen
| | Sample_contact_email | bryancp@umich.edu
| | Sample_contact_department | Pathology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 109 Zina Pitcher, 4620 BSRB
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48103
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM892nnn/GSM892320/suppl/GSM892320_Lukacs_010_J_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE36392
| | Sample_data_row_count | 45101
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