Result

Search results for the GEO ID: GSE36492

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM895819

GPL1261

Adipose tissue, normal diet

The adipose tissue of mice fed a normal diet (CE-2)

strain: C57BL/6 diet: normal diet (CE-2, as a control) tissue: adipose tissue

Gene expression data from adipose tissue obtained from mice fed a normal diet (ND) as a control

Sample_geo_accession	GSM895819
Sample_status	Public on Jan 30 2013
Sample_submission_date	Mar 14 2012
Sample_last_update_date	Jan 30 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were fed a normal diet CE-2 or high fat diet HFD32 (Clea, Tokyo, Japan) from 6 week-old to 18 week-old.
Sample_growth_protocol_ch1	Healthy grown C57BL/6 mice were used for high fat diet treatment to induce obesity.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	At 18 week-old, total RNAs from adipose tissues were prepared by using Nucleospin RNA II Isolation Kit (Macherey-Nagel) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol. (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Labelled samples were hybridized to the microarrays according to the manufacturer’s protocol with Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 45
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneArray Scanner 3000. Scanning was preformed as described elsewhere. (Irizarry et al, Nucleic Acids Res 31, e15, 2003)
Sample_data_processing	The intensity data was background-subtracted and normalized with the robust multi-array analysis (RMA; Irizarry et al. Nucleic Acids Res 31(4): e15, 2003) via R/Bioconductor affy 2.13.1 package using default analysis settings.
Sample_data_processing	The values of matrix table are logarithmic (log2) values
Sample_platform_id	GPL1261
Sample_contact_name	Yasushi,,Okazaki
Sample_contact_email	okazaki@saitama-med.ac.jp
Sample_contact_laboratory	Functional Genomics & Systems Medicine
Sample_contact_department	Research Center for Genomic Medicine
Sample_contact_institute	Saitama Medical University
Sample_contact_address	1397-1 Yamane
Sample_contact_city	Hidaka
Sample_contact_state	Saitama
Sample_contact_zip/postal_code	350-1241
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM895nnn/GSM895819/suppl/GSM895819_MOUSE_CD.CEL.gz
Sample_series_id	GSE36492
Sample_data_row_count	45101

GSM895820

GPL1261

Adipose tissue, high fat diet

The adipose tissue of mice fed a high fat diet (HFD32)

strain: C57BL/6 diet: high fat diet (HFD32) tissue: adipose tissue

Gene expression data from the adipose tissue obtained from mice fed a high fat diet (HFD).

Sample_geo_accession	GSM895820
Sample_status	Public on Jan 30 2013
Sample_submission_date	Mar 14 2012
Sample_last_update_date	Jan 30 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were fed a normal diet CE-2 or high fat diet HFD32 (Clea, Tokyo, Japan) from 6 week-old to 18 week-old.
Sample_growth_protocol_ch1	Healthy grown C57BL/6 mice were used for high fat diet treatment to induce obesity.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	At 18 week-old, total RNAs from adipose tissues were prepared by using Nucleospin RNA II Isolation Kit (Macherey-Nagel) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol. (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Labelled samples were hybridized to the microarrays according to the manufacturer’s protocol with Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 45
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneArray Scanner 3000. Scanning was preformed as described elsewhere. (Irizarry et al, Nucleic Acids Res 31, e15, 2003)
Sample_data_processing	The intensity data was background-subtracted and normalized with the robust multi-array analysis (RMA; Irizarry et al. Nucleic Acids Res 31(4): e15, 2003) via R/Bioconductor affy 2.13.1 package using default analysis settings.
Sample_data_processing	The values of matrix table are logarithmic (log2) values
Sample_platform_id	GPL1261
Sample_contact_name	Yasushi,,Okazaki
Sample_contact_email	okazaki@saitama-med.ac.jp
Sample_contact_laboratory	Functional Genomics & Systems Medicine
Sample_contact_department	Research Center for Genomic Medicine
Sample_contact_institute	Saitama Medical University
Sample_contact_address	1397-1 Yamane
Sample_contact_city	Hidaka
Sample_contact_state	Saitama
Sample_contact_zip/postal_code	350-1241
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM895nnn/GSM895820/suppl/GSM895820_MOUSE_HFD.CEL.gz
Sample_series_id	GSE36492
Sample_data_row_count	45101

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