Search results for the GEO ID: GSE36520  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM896078 | GPL1261 |
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CD103+ Th1
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primary CD4 T cell from spleen
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strain: C57BL/6
tissue: spleen
cell type: CD4 T cell
culture condition: CD103+ Th1
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Gene expression data from wild type CD4+ T cells cultured for 5 days in CD103+ Th1 conditions
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| Sample_geo_accession | GSM896078
| | Sample_status | Public on Mar 17 2012
| | Sample_submission_date | Mar 15 2012
| | Sample_last_update_date | Mar 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CD103+Th1 and Th1 cells were collected and resuspendet in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | Splenic CD4 T cells were prepared using a magnetic cell sorter (AutoMACS; Miltenyi Biotec) yielding a purity of >98%. Where indicated, cells from C57BL/6 mice were stimulated with immobilized anti-TCR mAb (H57−597; 3 mg/ml) and anti-CD28 mAb under CD103+ Th1- or Th1-culture conditions for 2 days in vitro. CD103+ Th1 conditions: IL-4 (3ng/ml), IFNgamma (10ng/ml), TGFbeta (3ng/ml). Th1 conditions: IL-12 (1ng/ml) and an anti-IL-4 mAb (11B11) (1μg/ml). After culturing for 3 more days, the cells were harvested.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was extracted using the IVT Labeling kit from Affymetrix
| | Sample_hyb_protocol | aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Expression values were determined using GeneChip Operating Software (GCOS) v1.2 software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Soichi,,Tofukuji
| | Sample_contact_laboratory | Laboratory of Medical Genomics
| | Sample_contact_department | Human Genome Research
| | Sample_contact_institute | Kazusa DNA Research Institute
| | Sample_contact_address | 2-6-7 Kazusa-kamatari
| | Sample_contact_city | Kisarazu
| | Sample_contact_zip/postal_code | 292-0818
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896078/suppl/GSM896078_EA0681_02.CEL.gz
| | Sample_series_id | GSE36520
| | Sample_series_id | GSE36556
| | Sample_data_row_count | 45037
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GSM896079 | GPL1261 |
|
Th1
|
primary CD4 T cell from spleen
|
strain: C57BL/6
tissue: spleen
cell type: CD4 T cell
culture condition: Th1
|
Gene expression data from wild type CD4+ T cells cultured for 5 days in Th1 conditions
|
| Sample_geo_accession | GSM896079
| | Sample_status | Public on Mar 17 2012
| | Sample_submission_date | Mar 15 2012
| | Sample_last_update_date | Mar 18 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | CD103+Th1 and Th1 cells were collected and resuspendet in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | Splenic CD4 T cells were prepared using a magnetic cell sorter (AutoMACS; Miltenyi Biotec) yielding a purity of >98%. Where indicated, cells from C57BL/6 mice were stimulated with immobilized anti-TCR mAb (H57−597; 3 mg/ml) and anti-CD28 mAb under CD103+ Th1- or Th1-culture conditions for 2 days in vitro. CD103+ Th1 conditions: IL-4 (3ng/ml), IFNgamma (10ng/ml), TGFbeta (3ng/ml). Th1 conditions: IL-12 (1ng/ml) and an anti-IL-4 mAb (11B11) (1μg/ml). After culturing for 3 more days, the cells were harvested.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was extracted using the IVT Labeling kit from Affymetrix
| | Sample_hyb_protocol | aRNA was hybridized to GeneChip Mouse Genome 430 2.0 Arrays from Affymetrix according the manufacturers protocol.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Expression values were determined using GeneChip Operating Software (GCOS) v1.2 software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Soichi,,Tofukuji
| | Sample_contact_laboratory | Laboratory of Medical Genomics
| | Sample_contact_department | Human Genome Research
| | Sample_contact_institute | Kazusa DNA Research Institute
| | Sample_contact_address | 2-6-7 Kazusa-kamatari
| | Sample_contact_city | Kisarazu
| | Sample_contact_zip/postal_code | 292-0818
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896079/suppl/GSM896079_EA0681_01.CEL.gz
| | Sample_series_id | GSE36520
| | Sample_series_id | GSE36556
| | Sample_data_row_count | 45037
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