Result

Search results for the GEO ID: GSE36530

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM896200

GPL1261

WT_STI_0Gy_Rep_1

Wild type v-abl-transformed pre-B cell

cell type: G1-phase pre-B cell line genotype/variation: v-abl-transformed

Gene expression from G1-phase pre-B cells

Sample_geo_accession	GSM896200
Sample_status	Public on Mar 13 2013
Sample_submission_date	Mar 15 2012
Sample_last_update_date	Mar 13 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media and incubated for 48 hr. Cells were irradiated at a rate of 0.72 Gy/min for a final dose of 1 Gy in a Cesium source irradiator and pelleted 2 hr following irradiation.
Sample_growth_protocol_ch1	Cells were maintained in suspension in Dulbecco's modified Eagle Medium (DMEM), high glucose, (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One microgram of total RNA was amplified as directed in the Affymetrix 3’ IVT Express kit protocol, performing the IVT reaction for 16 hours. Twelve and a half micrograms of amplified biotin-aRNAs were fragmented.
Sample_hyb_protocol	Ten micrograms of fragmented RNA were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Sample_scan_protocol	Arrays were scanned in an Affymetrix Scanner 3000.
Sample_data_processing	Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) and using the MAS5 algorithm to generate .CHP files.
Sample_platform_id	GPL1261
Sample_contact_name	NIEHS,,Microarray Core
Sample_contact_email	microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Sample_contact_laboratory	Microarray Core
Sample_contact_department	DIR
Sample_contact_institute	NIEHS
Sample_contact_address	111 T.W. Alexander Drive
Sample_contact_city	RTP
Sample_contact_state	NC
Sample_contact_zip/postal_code	27709
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896200/suppl/GSM896200_11010_WT_STI_0Gy_Rep_1_Mouse430_2_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896200/suppl/GSM896200_11010_WT_STI_0Gy_Rep_1_Mouse430_2_.mas5.CHP.gz
Sample_series_id	GSE36530
Sample_data_row_count	45101

GSM896201

GPL1261

WT_STI_1Gy_Rep_1

Irradiated wild type v-abl-transformed pre-B cell

cell type: G1-phase pre-B cell line genotype/variation: v-abl-transformed

Gene expression from irradiated G1-phase pre-B cells

Sample_geo_accession	GSM896201
Sample_status	Public on Mar 13 2013
Sample_submission_date	Mar 15 2012
Sample_last_update_date	Mar 13 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media and incubated for 48 hr. Cells were irradiated at a rate of 0.72 Gy/min for a final dose of 1 Gy in a Cesium source irradiator and pelleted 2 hr following irradiation.
Sample_growth_protocol_ch1	Cells were maintained in suspension in Dulbecco's modified Eagle Medium (DMEM), high glucose, (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One microgram of total RNA was amplified as directed in the Affymetrix 3’ IVT Express kit protocol, performing the IVT reaction for 16 hours. Twelve and a half micrograms of amplified biotin-aRNAs were fragmented.
Sample_hyb_protocol	Ten micrograms of fragmented RNA were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Sample_scan_protocol	Arrays were scanned in an Affymetrix Scanner 3000.
Sample_data_processing	Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) and using the MAS5 algorithm to generate .CHP files.
Sample_platform_id	GPL1261
Sample_contact_name	NIEHS,,Microarray Core
Sample_contact_email	microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Sample_contact_laboratory	Microarray Core
Sample_contact_department	DIR
Sample_contact_institute	NIEHS
Sample_contact_address	111 T.W. Alexander Drive
Sample_contact_city	RTP
Sample_contact_state	NC
Sample_contact_zip/postal_code	27709
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896201/suppl/GSM896201_11011_WT_STI_1Gy_Rep_1_Mouse430_2_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896201/suppl/GSM896201_11011_WT_STI_1Gy_Rep_1_Mouse430_2_.mas5.CHP.gz
Sample_series_id	GSE36530
Sample_data_row_count	45101

GSM896202

GPL1261

WT_STI_0Gy_Rep_2

Wild type v-abl-transformed pre-B cell

cell type: G1-phase pre-B cell line genotype/variation: v-abl-transformed

Gene expression from G1-phase pre-B cells

Sample_geo_accession	GSM896202
Sample_status	Public on Mar 13 2013
Sample_submission_date	Mar 15 2012
Sample_last_update_date	Mar 13 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media and incubated for 48 hr. Cells were irradiated at a rate of 0.72 Gy/min for a final dose of 1 Gy in a Cesium source irradiator and pelleted 2 hr following irradiation.
Sample_growth_protocol_ch1	Cells were maintained in suspension in Dulbecco's modified Eagle Medium (DMEM), high glucose, (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One microgram of total RNA was amplified as directed in the Affymetrix 3’ IVT Express kit protocol, performing the IVT reaction for 16 hours. Twelve and a half micrograms of amplified biotin-aRNAs were fragmented.
Sample_hyb_protocol	Ten micrograms of fragmented RNA were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Sample_scan_protocol	Arrays were scanned in an Affymetrix Scanner 3000.
Sample_data_processing	Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) and using the MAS5 algorithm to generate .CHP files.
Sample_platform_id	GPL1261
Sample_contact_name	NIEHS,,Microarray Core
Sample_contact_email	microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Sample_contact_laboratory	Microarray Core
Sample_contact_department	DIR
Sample_contact_institute	NIEHS
Sample_contact_address	111 T.W. Alexander Drive
Sample_contact_city	RTP
Sample_contact_state	NC
Sample_contact_zip/postal_code	27709
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896202/suppl/GSM896202_11014_WT_STI_0Gy_Rep_2_Mouse430_2_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896202/suppl/GSM896202_11014_WT_STI_0Gy_Rep_2_Mouse430_2_.mas5.CHP.gz
Sample_series_id	GSE36530
Sample_data_row_count	45101

GSM896203

GPL1261

WT_STI_1Gy_Rep_2

Irradiated wild type v-abl-transformed pre-B cell

cell type: G1-phase pre-B cell line genotype/variation: v-abl-transformed

Gene expression from irradiated G1-phase pre-B cells

Sample_geo_accession	GSM896203
Sample_status	Public on Mar 13 2013
Sample_submission_date	Mar 15 2012
Sample_last_update_date	Mar 13 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media and incubated for 48 hr. Cells were irradiated at a rate of 0.72 Gy/min for a final dose of 1 Gy in a Cesium source irradiator and pelleted 2 hr following irradiation.
Sample_growth_protocol_ch1	Cells were maintained in suspension in Dulbecco's modified Eagle Medium (DMEM), high glucose, (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One microgram of total RNA was amplified as directed in the Affymetrix 3’ IVT Express kit protocol, performing the IVT reaction for 16 hours. Twelve and a half micrograms of amplified biotin-aRNAs were fragmented.
Sample_hyb_protocol	Ten micrograms of fragmented RNA were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Sample_scan_protocol	Arrays were scanned in an Affymetrix Scanner 3000.
Sample_data_processing	Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) and using the MAS5 algorithm to generate .CHP files.
Sample_platform_id	GPL1261
Sample_contact_name	NIEHS,,Microarray Core
Sample_contact_email	microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Sample_contact_laboratory	Microarray Core
Sample_contact_department	DIR
Sample_contact_institute	NIEHS
Sample_contact_address	111 T.W. Alexander Drive
Sample_contact_city	RTP
Sample_contact_state	NC
Sample_contact_zip/postal_code	27709
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896203/suppl/GSM896203_11015_WT_STI_1Gy_Rep_2_Mouse430_2_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896203/suppl/GSM896203_11015_WT_STI_1Gy_Rep_2_Mouse430_2_.mas5.CHP.gz
Sample_series_id	GSE36530
Sample_data_row_count	45101

GSM896204

GPL1261

WT_STI_0Gy_Rep_3

Wild type v-abl-transformed pre-B cell

cell type: G1-phase pre-B cell line genotype/variation: v-abl-transformed

Gene expression from G1-phase pre-B cells

Sample_geo_accession	GSM896204
Sample_status	Public on Mar 13 2013
Sample_submission_date	Mar 15 2012
Sample_last_update_date	Mar 13 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media and incubated for 48 hr. Cells were irradiated at a rate of 0.72 Gy/min for a final dose of 1 Gy in a Cesium source irradiator and pelleted 2 hr following irradiation.
Sample_growth_protocol_ch1	Cells were maintained in suspension in Dulbecco's modified Eagle Medium (DMEM), high glucose, (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One microgram of total RNA was amplified as directed in the Affymetrix 3’ IVT Express kit protocol, performing the IVT reaction for 16 hours. Twelve and a half micrograms of amplified biotin-aRNAs were fragmented.
Sample_hyb_protocol	Ten micrograms of fragmented RNA were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Sample_scan_protocol	Arrays were scanned in an Affymetrix Scanner 3000.
Sample_data_processing	Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) and using the MAS5 algorithm to generate .CHP files.
Sample_platform_id	GPL1261
Sample_contact_name	NIEHS,,Microarray Core
Sample_contact_email	microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Sample_contact_laboratory	Microarray Core
Sample_contact_department	DIR
Sample_contact_institute	NIEHS
Sample_contact_address	111 T.W. Alexander Drive
Sample_contact_city	RTP
Sample_contact_state	NC
Sample_contact_zip/postal_code	27709
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896204/suppl/GSM896204_11018_WT_STI_0Gy_Rep_3_Mouse430_2_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896204/suppl/GSM896204_11018_WT_STI_0Gy_Rep_3_Mouse430_2_.mas5.CHP.gz
Sample_series_id	GSE36530
Sample_data_row_count	45101

GSM896205

GPL1261

WT_STI_1Gy_Rep_3

Irradiated wild type v-abl-transformed pre-B cell

cell type: G1-phase pre-B cell line genotype/variation: v-abl-transformed

Gene expression from irradiated G1-phase pre-B cells

Sample_geo_accession	GSM896205
Sample_status	Public on Mar 13 2013
Sample_submission_date	Mar 15 2012
Sample_last_update_date	Mar 13 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media and incubated for 48 hr. Cells were irradiated at a rate of 0.72 Gy/min for a final dose of 1 Gy in a Cesium source irradiator and pelleted 2 hr following irradiation.
Sample_growth_protocol_ch1	Cells were maintained in suspension in Dulbecco's modified Eagle Medium (DMEM), high glucose, (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One microgram of total RNA was amplified as directed in the Affymetrix 3’ IVT Express kit protocol, performing the IVT reaction for 16 hours. Twelve and a half micrograms of amplified biotin-aRNAs were fragmented.
Sample_hyb_protocol	Ten micrograms of fragmented RNA were hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Sample_scan_protocol	Arrays were scanned in an Affymetrix Scanner 3000.
Sample_data_processing	Data was obtained using the GeneChip® Command Console Software (AGCC; Version 1.1) and using the MAS5 algorithm to generate .CHP files.
Sample_platform_id	GPL1261
Sample_contact_name	NIEHS,,Microarray Core
Sample_contact_email	microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Sample_contact_laboratory	Microarray Core
Sample_contact_department	DIR
Sample_contact_institute	NIEHS
Sample_contact_address	111 T.W. Alexander Drive
Sample_contact_city	RTP
Sample_contact_state	NC
Sample_contact_zip/postal_code	27709
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896205/suppl/GSM896205_11019_WT_STI_1Gy_Rep_3_Mouse430_2_.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM896nnn/GSM896205/suppl/GSM896205_11019_WT_STI_1Gy_Rep_3_Mouse430_2_.mas5.CHP.gz
Sample_series_id	GSE36530
Sample_data_row_count	45101

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