Search results for the GEO ID: GSE36572  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM897007 | GPL570 |
|
A-204_Control
|
rhabdomyosacrcoma A-204 cells
|
cell line: rhabdomyosacrcoma A-204 cells
agent: untreated
|
Gene expression data from A-204 cells, untreated
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897007
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897007/suppl/GSM897007_A-204_Kontrolle.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897007/suppl/GSM897007_A-204_Kontrolle.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897008 | GPL570 |
|
A-204_T+T
|
rhabdomyosacrcoma A-204 cells
|
cell line: rhabdomyosacrcoma A-204 cells
agent: Trail and Taurolidine
|
Gene expression data from A-204 cells treated with Trail and Taurolidine
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897008
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897008/suppl/GSM897008_A-204_T+T.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897008/suppl/GSM897008_A-204_T+T.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897009 | GPL570 |
|
A-204_TRD
|
rhabdomyosacrcoma A-204 cells
|
cell line: rhabdomyosacrcoma A-204 cells
agent: Taurolidine
|
Gene expression data from A-204 cells treated with Taurolidine
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897009
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897009/suppl/GSM897009_A-204_Tauro.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897009/suppl/GSM897009_A-204_Tauro.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897010 | GPL570 |
|
A-204_Trail
|
rhabdomyosacrcoma A-204 cells
|
cell line: rhabdomyosacrcoma A-204 cells
agent: Trail
|
Gene expression data from A-204 cells treated with Trail
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897010
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897010/suppl/GSM897010_A-204_Trail.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897010/suppl/GSM897010_A-204_Trail.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897011 | GPL570 |
|
SK-LMS-1_Control
|
leiomyosarcoma SK-LMS-1 cells
|
cell line: leiomyosarcoma SK-LMS-1 cells
agent: untreated
|
Gene expression data from SK-LMS-1 cells, untreated
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897011
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897011/suppl/GSM897011_SK-LMS-1_Kontrolle.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897011/suppl/GSM897011_SK-LMS-1_Kontrolle.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897012 | GPL570 |
|
SK-LMS-1_T+T
|
leiomyosarcoma SK-LMS-1 cells
|
cell line: leiomyosarcoma SK-LMS-1 cells
agent: Trail and Taurolidine
|
Gene expression data from SK-LMS-1 cells treated with Trail and Taurolidine
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897012
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897012/suppl/GSM897012_SK-LMS-1_T+T.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897012/suppl/GSM897012_SK-LMS-1_T+T.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897013 | GPL570 |
|
SK-LMS-1_TRD
|
leiomyosarcoma SK-LMS-1 cells
|
cell line: leiomyosarcoma SK-LMS-1 cells
agent: Taurolidine
|
Gene expression data from SK-LMS-1 cells treated with Taurolidine
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897013
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897013/suppl/GSM897013_SK-LMS-1_Tauro.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897013/suppl/GSM897013_SK-LMS-1_Tauro.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897014 | GPL570 |
|
SK-LMS-1_Trail
|
leiomyosarcoma SK-LMS-1 cells
|
cell line: leiomyosarcoma SK-LMS-1 cells
agent: Trail
|
Gene expression data from SK-LMS-1 cells treated with Trail
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897014
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897014/suppl/GSM897014_SK-LMS-1_Trail.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897014/suppl/GSM897014_SK-LMS-1_Trail.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897015 | GPL570 |
|
VA-ES-BJ_Control
|
epithelioid sarcoma VA-ES-BJ cells
|
cell line: epithelioid sarcoma VA-ES-BJ cells
agent: untreated
|
Gene expression data from VA-ES-BJ cells, untreated
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897015
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897015/suppl/GSM897015_VA-ES-BJ_Kontrolle.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897015/suppl/GSM897015_VA-ES-BJ_Kontrolle.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897016 | GPL570 |
|
VA-ES-BJ_T+T
|
epithelioid sarcoma VA-ES-BJ cells
|
cell line: epithelioid sarcoma VA-ES-BJ cells
agent: Trail and Taurolidine
|
Gene expression data from VA-ES-BJ cells treated with Trail and Taurolidine
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897016
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897016/suppl/GSM897016_VA-ES-BJ_T+T.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897016/suppl/GSM897016_VA-ES-BJ_T+T.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897017 | GPL570 |
|
VA-ES-BJ_TRD
|
epithelioid sarcoma VA-ES-BJ cells
|
cell line: epithelioid sarcoma VA-ES-BJ cells
agent: Taurolidine
|
Gene expression data from VA-ES-BJ cells treated with Taurolidine
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897017
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897017/suppl/GSM897017_VA-ES-BJ_Tauro.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897017/suppl/GSM897017_VA-ES-BJ_Tauro.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
GSM897018 | GPL570 |
|
VA-ES-BJ_Trail
|
epithelioid sarcoma VA-ES-BJ cells
|
cell line: epithelioid sarcoma VA-ES-BJ cells
agent: Trail
|
Gene expression data from VA-ES-BJ cells treated with Trail
total RNA (>200 nt)
|
| Sample_geo_accession | GSM897018
| | Sample_status | Public on Mar 17 2013
| | Sample_submission_date | Mar 16 2012
| | Sample_last_update_date | Mar 17 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For every drug experiment, 80 µl of 3.125 × 106 cells/ml were placed in 6-well plates containing the medium. After 24 hours, the medium was replaced and the drugs were added to each well. The cultivated soft tissue sarcoma cells were incubated with TRD (250 μmol/l) and/or TRAIL (50 ng/ml) for 6 hours.
| | Sample_growth_protocol_ch1 | Rhabdomyosarcoma (A-204) and leiomyosarcoma (SK-LMS-1) and epithelioid cell sarcoma (VA-ES-BJ) cells were maintained in modified Eagle's medium (MEM) and NEAA (non-essential amino acids) + 10% FBS (foetal bovine serum) supplemented with 1% penicillin (100 U/ml) and streptomycin (100 μg/ ml), 1% sodium pyruvate and 1% L-glutamine. The cells were cultured in a humidified atmosphere with 5% CO2 at 37°C .
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA isolation from cell lines was performed using standard acid guanidinium thiocyanate-phenolchloroform extraction procedure. Total-RNA concentration and purity of total RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix 3'IVT protocol starting from 4-5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in the Affymetrix GeneChip Scanner 3000 with G7 update.
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897018/suppl/GSM897018_VA-ES-BJ_Trail.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897018/suppl/GSM897018_VA-ES-BJ_Trail.CHP.gz
| | Sample_series_id | GSE36572
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
