Search results for the GEO ID: GSE36591 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM897234 | GPL1261 |
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EA0703_01
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uninephrectomy alone mice
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tissue: kidney removed 6week after the uninephrectomy
gender: Male
strain: C57BL/6J mice
age: 16 weeks old
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Sample_geo_accession | GSM897234
| Sample_status | Public on Mar 19 2012
| Sample_submission_date | Mar 18 2012
| Sample_last_update_date | Mar 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cardio-renal syndrome mice were established by the combination of abdominal aortic banding and uninephrectomy. Abdominal aortic banding was conducted by constricting the abdominal aorta under the divergence of bilateral renal arteries. Unilateral nephrectomy was conducted 2 weeks after the aortic banding.
| Sample_growth_protocol_ch1 | Mice were maintained in our animal facility. Animal care was in accordance with the guidelines of Niigata University.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Each remaining kidney in uninephrectomy alone mice or cardio-renal syndrome mice was removed to purify total RNA 6 weeks after the uninephrectomy. Total RNA was extracted by using RNAiso (Takara Bio, Japan) which use AGPC(acid guanidinium thiocyanate-phenol-chloroform extraction) method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by GeneChip 3'IVT Express Kit(Affymetrix) from 250 ng total RNA according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL1261
| Sample_contact_name | yohei,,Tsuchida
| Sample_contact_email | yoheyyohei@yahoo.co.jp
| Sample_contact_institute | Niigata University Graduate School of Medical and Dental Sciences
| Sample_contact_address | 1-757 Asahimachi-dori
| Sample_contact_city | Niigata
| Sample_contact_zip/postal_code | 951-8510
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897234/suppl/GSM897234_EA0703_01.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897234/suppl/GSM897234_EA0703_01_scale.CHP.gz
| Sample_series_id | GSE36591
| Sample_data_row_count | 45101
| |
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GSM897235 | GPL1261 |
|
EA0703_02
|
cardio-renal syndrome mice
|
tissue: kidney removed 6week after the uninephrectomy
gender: Male
strain: C57BL/6J mice
age: 16 weeks old
|
|
Sample_geo_accession | GSM897235
| Sample_status | Public on Mar 19 2012
| Sample_submission_date | Mar 18 2012
| Sample_last_update_date | Mar 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cardio-renal syndrome mice were established by the combination of abdominal aortic banding and uninephrectomy. Abdominal aortic banding was conducted by constricting the abdominal aorta under the divergence of bilateral renal arteries. Unilateral nephrectomy was conducted 2 weeks after the aortic banding.
| Sample_growth_protocol_ch1 | Mice were maintained in our animal facility. Animal care was in accordance with the guidelines of Niigata University.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Each remaining kidney in uninephrectomy alone mice or cardio-renal syndrome mice was removed to purify total RNA 6 weeks after the uninephrectomy. Total RNA was extracted by using RNAiso (Takara Bio, Japan) which use AGPC(acid guanidinium thiocyanate-phenol-chloroform extraction) method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by GeneChip 3'IVT Express Kit(Affymetrix) from 250 ng total RNA according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL1261
| Sample_contact_name | yohei,,Tsuchida
| Sample_contact_email | yoheyyohei@yahoo.co.jp
| Sample_contact_institute | Niigata University Graduate School of Medical and Dental Sciences
| Sample_contact_address | 1-757 Asahimachi-dori
| Sample_contact_city | Niigata
| Sample_contact_zip/postal_code | 951-8510
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897235/suppl/GSM897235_EA0703_02.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897235/suppl/GSM897235_EA0703_02_scale.CHP.gz
| Sample_series_id | GSE36591
| Sample_data_row_count | 45101
| |
|
GSM897236 | GPL1261 |
|
EA0703_03
|
cardio-renal syndrome mice
|
tissue: kidney removed 6week after the uninephrectomy
gender: Male
strain: C57BL/6J mice
age: 16 weeks old
|
|
Sample_geo_accession | GSM897236
| Sample_status | Public on Mar 19 2012
| Sample_submission_date | Mar 18 2012
| Sample_last_update_date | Mar 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cardio-renal syndrome mice were established by the combination of abdominal aortic banding and uninephrectomy. Abdominal aortic banding was conducted by constricting the abdominal aorta under the divergence of bilateral renal arteries. Unilateral nephrectomy was conducted 2 weeks after the aortic banding.
| Sample_growth_protocol_ch1 | Mice were maintained in our animal facility. Animal care was in accordance with the guidelines of Niigata University.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Each remaining kidney in uninephrectomy alone mice or cardio-renal syndrome mice was removed to purify total RNA 6 weeks after the uninephrectomy. Total RNA was extracted by using RNAiso (Takara Bio, Japan) which use AGPC(acid guanidinium thiocyanate-phenol-chloroform extraction) method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared by GeneChip 3'IVT Express Kit(Affymetrix) from 250 ng total RNA according to the manufacturer's instructions.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL1261
| Sample_contact_name | yohei,,Tsuchida
| Sample_contact_email | yoheyyohei@yahoo.co.jp
| Sample_contact_institute | Niigata University Graduate School of Medical and Dental Sciences
| Sample_contact_address | 1-757 Asahimachi-dori
| Sample_contact_city | Niigata
| Sample_contact_zip/postal_code | 951-8510
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897236/suppl/GSM897236_EA0703_03.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897236/suppl/GSM897236_EA0703_03_scale.CHP.gz
| Sample_series_id | GSE36591
| Sample_data_row_count | 45101
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