Search results for the GEO ID: GSE36618  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM897562 | GPL1261 |
|
Wild type, replicate 1
|
Normal fetal liver 1
|
strain: C57BL/6
age: 13.5 day old fetus
genotype/variation: wild-type
tissue: liver
cell type: early erythroid progenitors
|
|
| Sample_geo_accession | GSM897562
| | Sample_status | Public on Mar 20 2012
| | Sample_submission_date | Mar 20 2012
| | Sample_last_update_date | Mar 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fetal livers from day 13.5 wild type and Eklf -/- embryos were dissociated to a single cell suspension and stained with 7AAD, anti-CD71 and Ter119 antibodies. Early erythroid progenitor cell populations were isolated using a FACSAria flow cytometer running FACSDiva software.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared from early erythroid progenitor cells sorted from day 13.5 wild type and Eklf -/- fetal liver cells using TRIzol reagent. RNA quality was verified with an Agilent 2100 Bioanalyzer. GeneChip target probes from 3 independent Eklf -/- and 3 wild type RNA samples were prepared according to Affymetrix’ instructions starting with 10μg of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Prepared samples using the Affymetrix GeneChip ENZO labeling kit
| | Sample_hyb_protocol | Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
| | Sample_scan_protocol | Scanning of Affymetrix GeneChip arrays was performed according to the Affymerix manual instructions
| | Sample_data_processing | MAS 5.0 data was performed in the Affymetrix GeneChip Expression Console
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Holly,Kloos,Dressman
| | Sample_contact_email | dress002@mc.duke.edu
| | Sample_contact_department | Institute for Genome Sciences and Policy
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | 101 Science Drive
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27708
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897562/suppl/GSM897562_0103_8656_M430-2_TER01.CEL.gz
| | Sample_series_id | GSE36618
| | Sample_data_row_count | 45101
| | |
|
GSM897563 | GPL1261 |
|
Wild type, replicate 2
|
Normal fetal liver 2
|
strain: C57BL/6
age: 13.5 day old fetus
genotype/variation: wild-type
tissue: liver
cell type: early erythroid progenitors
|
|
| Sample_geo_accession | GSM897563
| | Sample_status | Public on Mar 20 2012
| | Sample_submission_date | Mar 20 2012
| | Sample_last_update_date | Mar 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fetal livers from day 13.5 wild type and Eklf -/- embryos were dissociated to a single cell suspension and stained with 7AAD, anti-CD71 and Ter119 antibodies. Early erythroid progenitor cell populations were isolated using a FACSAria flow cytometer running FACSDiva software.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared from early erythroid progenitor cells sorted from day 13.5 wild type and Eklf -/- fetal liver cells using TRIzol reagent. RNA quality was verified with an Agilent 2100 Bioanalyzer. GeneChip target probes from 3 independent Eklf -/- and 3 wild type RNA samples were prepared according to Affymetrix’ instructions starting with 10μg of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Prepared samples using the Affymetrix GeneChip ENZO labeling kit
| | Sample_hyb_protocol | Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
| | Sample_scan_protocol | Scanning of Affymetrix GeneChip arrays was performed according to the Affymerix manual instructions
| | Sample_data_processing | MAS 5.0 data was performed in the Affymetrix GeneChip Expression Console
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Holly,Kloos,Dressman
| | Sample_contact_email | dress002@mc.duke.edu
| | Sample_contact_department | Institute for Genome Sciences and Policy
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | 101 Science Drive
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27708
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897563/suppl/GSM897563_0103_8657_M430-2_TER02.CEL.gz
| | Sample_series_id | GSE36618
| | Sample_data_row_count | 45101
| | |
|
GSM897564 | GPL1261 |
|
Wild type, replicate 3
|
Normal fetal liver 3
|
strain: C57BL/6
age: 13.5 day old fetus
genotype/variation: wild-type
tissue: liver
cell type: early erythroid progenitors
|
|
| Sample_geo_accession | GSM897564
| | Sample_status | Public on Mar 20 2012
| | Sample_submission_date | Mar 20 2012
| | Sample_last_update_date | Mar 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fetal livers from day 13.5 wild type and Eklf -/- embryos were dissociated to a single cell suspension and stained with 7AAD, anti-CD71 and Ter119 antibodies. Early erythroid progenitor cell populations were isolated using a FACSAria flow cytometer running FACSDiva software.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared from early erythroid progenitor cells sorted from day 13.5 wild type and Eklf -/- fetal liver cells using TRIzol reagent. RNA quality was verified with an Agilent 2100 Bioanalyzer. GeneChip target probes from 3 independent Eklf -/- and 3 wild type RNA samples were prepared according to Affymetrix’ instructions starting with 10μg of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Prepared samples using the Affymetrix GeneChip ENZO labeling kit
| | Sample_hyb_protocol | Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
| | Sample_scan_protocol | Scanning of Affymetrix GeneChip arrays was performed according to the Affymerix manual instructions
| | Sample_data_processing | MAS 5.0 data was performed in the Affymetrix GeneChip Expression Console
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Holly,Kloos,Dressman
| | Sample_contact_email | dress002@mc.duke.edu
| | Sample_contact_department | Institute for Genome Sciences and Policy
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | 101 Science Drive
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27708
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897564/suppl/GSM897564_0103_8658_M430-2_TER03.CEL.gz
| | Sample_series_id | GSE36618
| | Sample_data_row_count | 45101
| | |
|
GSM897565 | GPL1261 |
|
EKLF, replicate 1
|
EKLF -/- fetal liver 1
|
strain: C57BL/6
age: 13.5 day old fetus
genotype/variation: EKLF knock out
tissue: liver
cell type: early erythroid progenitors
|
The EKLF knock out mice were referenced in our manuscript
(#34) Perkins, A. C., A. H. Sharpe, and S. H. Orkin. 1995. Lethal beta-thalassaemia in mice lacking the erythroid CACCC-transcription factor EKLF.
Nature 375:318 322.
From this paper:
The EKLF genomic clones were isolated from a lambdaFiXII mouse strain 129 library.......and targeting clones were injected into C57BL/6 blastocysts, which were transferred to the uterus in pseudopregnant BALB/c Swiss mice.
|
| Sample_geo_accession | GSM897565
| | Sample_status | Public on Mar 20 2012
| | Sample_submission_date | Mar 20 2012
| | Sample_last_update_date | Mar 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fetal livers from day 13.5 wild type and Eklf -/- embryos were dissociated to a single cell suspension and stained with 7AAD, anti-CD71 and Ter119 antibodies. Early erythroid progenitor cell populations were isolated using a FACSAria flow cytometer running FACSDiva software.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared from early erythroid progenitor cells sorted from day 13.5 wild type and Eklf -/- fetal liver cells using TRIzol reagent. RNA quality was verified with an Agilent 2100 Bioanalyzer. GeneChip target probes from 3 independent Eklf -/- and 3 wild type RNA samples were prepared according to Affymetrix’ instructions starting with 10μg of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Prepared samples using the Affymetrix GeneChip ENZO labeling kit
| | Sample_hyb_protocol | Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
| | Sample_scan_protocol | Scanning of Affymetrix GeneChip arrays was performed according to the Affymerix manual instructions
| | Sample_data_processing | MAS 5.0 data was performed in the Affymetrix GeneChip Expression Console
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Holly,Kloos,Dressman
| | Sample_contact_email | dress002@mc.duke.edu
| | Sample_contact_department | Institute for Genome Sciences and Policy
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | 101 Science Drive
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27708
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897565/suppl/GSM897565_0103_6361_M430-2_EKLF01.CEL.gz
| | Sample_series_id | GSE36618
| | Sample_data_row_count | 45101
| | |
|
GSM897566 | GPL1261 |
|
EKLF, replicate 2
|
EKLF -/- fetal liver 2
|
strain: C57BL/6
age: 13.5 day old fetus
genotype/variation: EKLF knock out
tissue: liver
cell type: early erythroid progenitors
|
The EKLF knock out mice were referenced in our manuscript
(#34) Perkins, A. C., A. H. Sharpe, and S. H. Orkin. 1995. Lethal beta-thalassaemia in mice lacking the erythroid CACCC-transcription factor EKLF.
Nature 375:318 322.
From this paper:
The EKLF genomic clones were isolated from a lambdaFiXII mouse strain 129 library.......and targeting clones were injected into C57BL/6 blastocysts, which were transferred to the uterus in pseudopregnant BALB/c Swiss mice.
|
| Sample_geo_accession | GSM897566
| | Sample_status | Public on Mar 20 2012
| | Sample_submission_date | Mar 20 2012
| | Sample_last_update_date | Mar 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fetal livers from day 13.5 wild type and Eklf -/- embryos were dissociated to a single cell suspension and stained with 7AAD, anti-CD71 and Ter119 antibodies. Early erythroid progenitor cell populations were isolated using a FACSAria flow cytometer running FACSDiva software.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared from early erythroid progenitor cells sorted from day 13.5 wild type and Eklf -/- fetal liver cells using TRIzol reagent. RNA quality was verified with an Agilent 2100 Bioanalyzer. GeneChip target probes from 3 independent Eklf -/- and 3 wild type RNA samples were prepared according to Affymetrix’ instructions starting with 10μg of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Prepared samples using the Affymetrix GeneChip ENZO labeling kit
| | Sample_hyb_protocol | Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
| | Sample_scan_protocol | Scanning of Affymetrix GeneChip arrays was performed according to the Affymerix manual instructions
| | Sample_data_processing | MAS 5.0 data was performed in the Affymetrix GeneChip Expression Console
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Holly,Kloos,Dressman
| | Sample_contact_email | dress002@mc.duke.edu
| | Sample_contact_department | Institute for Genome Sciences and Policy
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | 101 Science Drive
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27708
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897566/suppl/GSM897566_0103_6344_M430-2_EKLF02.CEL.gz
| | Sample_series_id | GSE36618
| | Sample_data_row_count | 45101
| | |
|
GSM897567 | GPL1261 |
|
EKLF, replicate 3
|
EKLF -/- fetal liver 3
|
strain: C57BL/6
age: 13.5 day old fetus
genotype/variation: EKLF knock out
tissue: liver
cell type: early erythroid progenitors
|
The EKLF knock out mice were referenced in our manuscript
(#34) Perkins, A. C., A. H. Sharpe, and S. H. Orkin. 1995. Lethal beta-thalassaemia in mice lacking the erythroid CACCC-transcription factor EKLF.
Nature 375:318 322.
From this paper:
The EKLF genomic clones were isolated from a lambdaFiXII mouse strain 129 library.......and targeting clones were injected into C57BL/6 blastocysts, which were transferred to the uterus in pseudopregnant BALB/c Swiss mice.
|
| Sample_geo_accession | GSM897567
| | Sample_status | Public on Mar 20 2012
| | Sample_submission_date | Mar 20 2012
| | Sample_last_update_date | Mar 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fetal livers from day 13.5 wild type and Eklf -/- embryos were dissociated to a single cell suspension and stained with 7AAD, anti-CD71 and Ter119 antibodies. Early erythroid progenitor cell populations were isolated using a FACSAria flow cytometer running FACSDiva software.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was prepared from early erythroid progenitor cells sorted from day 13.5 wild type and Eklf -/- fetal liver cells using TRIzol reagent. RNA quality was verified with an Agilent 2100 Bioanalyzer. GeneChip target probes from 3 independent Eklf -/- and 3 wild type RNA samples were prepared according to Affymetrix’ instructions starting with 10μg of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Prepared samples using the Affymetrix GeneChip ENZO labeling kit
| | Sample_hyb_protocol | Hybridization of samples to Affymetrix GeneChip arrays was performed according to Affymetrix manual instructions
| | Sample_scan_protocol | Scanning of Affymetrix GeneChip arrays was performed according to the Affymerix manual instructions
| | Sample_data_processing | MAS 5.0 data was performed in the Affymetrix GeneChip Expression Console
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Holly,Kloos,Dressman
| | Sample_contact_email | dress002@mc.duke.edu
| | Sample_contact_department | Institute for Genome Sciences and Policy
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | 101 Science Drive
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27708
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM897nnn/GSM897567/suppl/GSM897567_0103_6345_M430-2_EKLF03.CEL.gz
| | Sample_series_id | GSE36618
| | Sample_data_row_count | 45101
| | |
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