Sample_geo_accession | GSM701542
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Sample_status | Public on Sep 10 2011
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Sample_submission_date | Apr 04 2011
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Sample_last_update_date | Mar 21 2012
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Homo sapiens
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Sample_taxid_ch1 | 9606
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Sample_treatment_protocol_ch1 | Cells were washed with PBS and Rneasy kit was used to isolate total RNA.
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Sample_growth_protocol_ch1 | iPSC were cultured in hES medium containing bFGF.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total RNA samples were isolated using RNeasy kit.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Approximately 150 ng of RNA from each sample was labeled using GeneChip 3’IVT Express (Affymetrix) according to the manufacturer’s instructions.
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Sample_hyb_protocol | Following fragmentation, all arrays were hybridized at 45C for 16 hr.
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Sample_scan_protocol | GeneChips were scanned using an AFX GC3000 G7 scanner.
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Sample_data_processing | The gene expression raw data were extracted using the AFFX Gene Chip Operation System. Quality control was performed on the basis of Affymetrix quality control metrics. The data were analyzed with the Gene Spring GX 11.0 (Agilent).
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Sample_platform_id | GPL570
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Sample_contact_name | Takuya,,Yagi
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Sample_contact_email | takuyagi@2006.jukuin.keio.ac.jp
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Sample_contact_institute | Keio University
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Sample_contact_address | 35-Shinanomachi Shinjuku-ku
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Sample_contact_city | Tokyo
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Sample_contact_zip/postal_code | 160-8582
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Sample_contact_country | Japan
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM701nnn/GSM701542/suppl/GSM701542_201B7.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM701nnn/GSM701542/suppl/GSM701542_201B7.CHP.gz
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Sample_series_id | GSE28379
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Sample_series_id | GSE36667
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Sample_data_row_count | 54675
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