Search results for the GEO ID: GSE36688  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM898813 | GPL1261 |
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K14CREER/RosaYFP 3.5days 1
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K14CREER/RosaYFP+
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background/strain: CD-1
genotype: K14CREER/RosaYFP
tissue: interfollicular epidermis (IFE)
cell type: alpha6 integrin-high/CD34-neg basal cells
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A1396_07
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| Sample_geo_accession | GSM898813
| | Sample_status | Public on Sep 07 2012
| | Sample_submission_date | Mar 21 2012
| | Sample_last_update_date | Sep 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | K14-CREER/rosa-YFP and Involucrin-CREER/Rosa-YFP mice were induced with 1ug and 10ug of tamoxifen, respectively, by IP injection and sacrificed 4 days later.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy microkit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies), and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 100 ng of total RNA spiked with bacterial RNA transcript positive controls (Affymetrix) was amplified and labeled using the GeneChip 3' IVT express kit (Affymetrix). All steps were carried out according to the manufacturer's protocol (Affymetrix).
| | Sample_hyb_protocol | A mixture of purified and fragmented biotinylated aRNA and hybridisation controls (Affymetrix) was hybridised on Affymetrix MOE 430 2.0 arrays, followed by staining and washing in a GeneChip® Fluidics Station 450 (Affymetrix) according to the manufacturer’s procedures.
| | Sample_scan_protocol | To assess the raw probe signal intensities, chips were scanned using a GeneChip® Scanner 3000 7G (Affymetrix, software AGCC v3.1.1.1229).
| | Sample_data_processing | Affymetrix Expression Console software v1.1. For normalization: RMA values in log2.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Cédric,,Blanpain
| | Sample_contact_email | cedric.blanpain@ulb.ac.be
| | Sample_contact_institute | IRIBHM
| | Sample_contact_address | route de Lennik 808
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM898nnn/GSM898813/suppl/GSM898813_A1396_07.CEL.gz
| | Sample_series_id | GSE36688
| | Sample_data_row_count | 45101
| | |
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GSM898814 | GPL1261 |
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K14CREER/RosaYFP 3.5days 2
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K14CREER/RosaYFP+
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background/strain: CD-1
genotype: K14CREER/RosaYFP
tissue: interfollicular epidermis (IFE)
cell type: alpha6 integrin-high/CD34-neg basal cells
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A1396_10
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| Sample_geo_accession | GSM898814
| | Sample_status | Public on Sep 07 2012
| | Sample_submission_date | Mar 21 2012
| | Sample_last_update_date | Sep 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | K14-CREER/rosa-YFP and Involucrin-CREER/Rosa-YFP mice were induced with 1ug and 10ug of tamoxifen, respectively, by IP injection and sacrificed 4 days later.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy microkit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies), and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 100 ng of total RNA spiked with bacterial RNA transcript positive controls (Affymetrix) was amplified and labeled using the GeneChip 3' IVT express kit (Affymetrix). All steps were carried out according to the manufacturer's protocol (Affymetrix).
| | Sample_hyb_protocol | A mixture of purified and fragmented biotinylated aRNA and hybridisation controls (Affymetrix) was hybridised on Affymetrix MOE 430 2.0 arrays, followed by staining and washing in a GeneChip® Fluidics Station 450 (Affymetrix) according to the manufacturer’s procedures.
| | Sample_scan_protocol | To assess the raw probe signal intensities, chips were scanned using a GeneChip® Scanner 3000 7G (Affymetrix, software AGCC v3.1.1.1229).
| | Sample_data_processing | Affymetrix Expression Console software v1.1. For normalization: RMA values in log2.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Cédric,,Blanpain
| | Sample_contact_email | cedric.blanpain@ulb.ac.be
| | Sample_contact_institute | IRIBHM
| | Sample_contact_address | route de Lennik 808
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM898nnn/GSM898814/suppl/GSM898814_A1396_10.CEL.gz
| | Sample_series_id | GSE36688
| | Sample_data_row_count | 45101
| | |
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GSM898815 | GPL1261 |
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InvCREER/RosaYFP 3.5days 1
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InvCREER/RosaYFP+
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background/strain: CD-1
genotype: InvCREER/RosaYFP
tissue: interfollicular epidermis (IFE)
cell type: alpha6 integrin-high/CD34-neg basal cells
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A1396_08
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| Sample_geo_accession | GSM898815
| | Sample_status | Public on Sep 07 2012
| | Sample_submission_date | Mar 21 2012
| | Sample_last_update_date | Sep 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | K14-CREER/rosa-YFP and Involucrin-CREER/Rosa-YFP mice were induced with 1ug and 10ug of tamoxifen, respectively, by IP injection and sacrificed 4 days later.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy microkit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies), and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 100 ng of total RNA spiked with bacterial RNA transcript positive controls (Affymetrix) was amplified and labeled using the GeneChip 3' IVT express kit (Affymetrix). All steps were carried out according to the manufacturer's protocol (Affymetrix).
| | Sample_hyb_protocol | A mixture of purified and fragmented biotinylated aRNA and hybridisation controls (Affymetrix) was hybridised on Affymetrix MOE 430 2.0 arrays, followed by staining and washing in a GeneChip® Fluidics Station 450 (Affymetrix) according to the manufacturer’s procedures.
| | Sample_scan_protocol | To assess the raw probe signal intensities, chips were scanned using a GeneChip® Scanner 3000 7G (Affymetrix, software AGCC v3.1.1.1229).
| | Sample_data_processing | Affymetrix Expression Console software v1.1. For normalization: RMA values in log2.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Cédric,,Blanpain
| | Sample_contact_email | cedric.blanpain@ulb.ac.be
| | Sample_contact_institute | IRIBHM
| | Sample_contact_address | route de Lennik 808
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM898nnn/GSM898815/suppl/GSM898815_A1396_08.CEL.gz
| | Sample_series_id | GSE36688
| | Sample_data_row_count | 45101
| | |
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GSM898816 | GPL1261 |
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InvCREER/RosaYFP 3.5days 2
|
InvCREER/RosaYFP+
|
background/strain: CD-1
genotype: InvCREER/RosaYFP
tissue: interfollicular epidermis (IFE)
cell type: alpha6 integrin-high/CD34-neg basal cells
|
A1396_11
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| Sample_geo_accession | GSM898816
| | Sample_status | Public on Sep 07 2012
| | Sample_submission_date | Mar 21 2012
| | Sample_last_update_date | Sep 07 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | K14-CREER/rosa-YFP and Involucrin-CREER/Rosa-YFP mice were induced with 1ug and 10ug of tamoxifen, respectively, by IP injection and sacrificed 4 days later.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy microkit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies), and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 100 ng of total RNA spiked with bacterial RNA transcript positive controls (Affymetrix) was amplified and labeled using the GeneChip 3' IVT express kit (Affymetrix). All steps were carried out according to the manufacturer's protocol (Affymetrix).
| | Sample_hyb_protocol | A mixture of purified and fragmented biotinylated aRNA and hybridisation controls (Affymetrix) was hybridised on Affymetrix MOE 430 2.0 arrays, followed by staining and washing in a GeneChip® Fluidics Station 450 (Affymetrix) according to the manufacturer’s procedures.
| | Sample_scan_protocol | To assess the raw probe signal intensities, chips were scanned using a GeneChip® Scanner 3000 7G (Affymetrix, software AGCC v3.1.1.1229).
| | Sample_data_processing | Affymetrix Expression Console software v1.1. For normalization: RMA values in log2.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Cédric,,Blanpain
| | Sample_contact_email | cedric.blanpain@ulb.ac.be
| | Sample_contact_institute | IRIBHM
| | Sample_contact_address | route de Lennik 808
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM898nnn/GSM898816/suppl/GSM898816_A1396_11.CEL.gz
| | Sample_series_id | GSE36688
| | Sample_data_row_count | 45101
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