Search results for the GEO ID: GSE36782  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM900983 | GPL570 |
|
NNI-1 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-1
|
| Sample_geo_accession | GSM900983
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900983/suppl/GSM900983_AFG1133-1.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900984 | GPL570 |
|
NNI-2 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-2
|
| Sample_geo_accession | GSM900984
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900984/suppl/GSM900984_AFG1133-2.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900985 | GPL570 |
|
NNI-3 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-3
|
| Sample_geo_accession | GSM900985
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900985/suppl/GSM900985_AFG1133-3.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900986 | GPL570 |
|
NNI-4 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-4
|
| Sample_geo_accession | GSM900986
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900986/suppl/GSM900986_AFG1133-4.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900987 | GPL570 |
|
NNI-5 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-5
|
| Sample_geo_accession | GSM900987
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900987/suppl/GSM900987_AFG1133-5.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900988 | GPL570 |
|
NNI-8 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Anaplastic oligoastrocytoma
grade: III
|
Date: Feb 1, 2011
AFG1133-6
|
| Sample_geo_accession | GSM900988
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900988/suppl/GSM900988_AFG1133-6.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900989 | GPL570 |
|
NNI-9 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-7
|
| Sample_geo_accession | GSM900989
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900989/suppl/GSM900989_AFG1133-7.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900990 | GPL570 |
|
NNI-10 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-8
|
| Sample_geo_accession | GSM900990
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900990/suppl/GSM900990_AFG1133-8.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900991 | GPL570 |
|
NNI-11 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-9
|
| Sample_geo_accession | GSM900991
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900991/suppl/GSM900991_AFG1133-9.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900992 | GPL570 |
|
NNI-12 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-10
|
| Sample_geo_accession | GSM900992
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900992/suppl/GSM900992_AFG1133-10.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900993 | GPL570 |
|
NNI-13 rep1
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-11
|
| Sample_geo_accession | GSM900993
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900993/suppl/GSM900993_AFG1133-11.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900994 | GPL570 |
|
NNI-1 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-22
|
| Sample_geo_accession | GSM900994
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900994/suppl/GSM900994_AFG1133-22.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900995 | GPL570 |
|
NNI-2 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-23
|
| Sample_geo_accession | GSM900995
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900995/suppl/GSM900995_AFG1133-23.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900996 | GPL570 |
|
NNI-3 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-24
|
| Sample_geo_accession | GSM900996
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900996/suppl/GSM900996_AFG1133-24.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900997 | GPL570 |
|
NNI-4 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-25
|
| Sample_geo_accession | GSM900997
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900997/suppl/GSM900997_AFG1133-25.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900998 | GPL570 |
|
NNI-5 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-26
|
| Sample_geo_accession | GSM900998
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900998/suppl/GSM900998_AFG1133-26.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM900999 | GPL570 |
|
NNI-8 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Anaplastic oligoastrocytoma
grade: III
|
Date: Feb 1, 2011
AFG1133-27
|
| Sample_geo_accession | GSM900999
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM900nnn/GSM900999/suppl/GSM900999_AFG1133-27.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM901000 | GPL570 |
|
NNI-9 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-28
|
| Sample_geo_accession | GSM901000
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM901nnn/GSM901000/suppl/GSM901000_AFG1133-28.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM901001 | GPL570 |
|
NNI-10 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-29
|
| Sample_geo_accession | GSM901001
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM901nnn/GSM901001/suppl/GSM901001_AFG1133-29.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
| | |
|
GSM901003 | GPL570 |
|
NNI-12 rep2
|
Primary neurosphere
|
tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
|
Date: Feb 1, 2011
AFG1133-31
|
| Sample_geo_accession | GSM901003
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM901nnn/GSM901003/suppl/GSM901003_AFG1133-31.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
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GSM901004 | GPL570 |
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NNI-13 rep2
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Primary neurosphere
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tissue: brain tumor
histology: Glioblastoma multiforme
grade: IV
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Date: Feb 1, 2011
AFG1133-32
|
| Sample_geo_accession | GSM901004
| | Sample_status | Public on Aug 02 2012
| | Sample_submission_date | Mar 25 2012
| | Sample_last_update_date | Aug 02 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Graded brain tumor specimens were obtained with informed consent, as part of a study protocol approved by the SingHealth Centralised Institutional Review Board A. GPC lines were described in our previous work.5 Briefly, cells were seeded as free-floating spheres at a density of 2,500 cells per cm2 in chemically defined serum-free growth medium containing basic fibroblast growth factor (bFGF; 20 ng/ml; PeproTech, New Jersey), epidermal growth factor (EGF; 20 ng/ml; PeproTech), heparin (5 ug/ml; Sigma-Aldrich, St Louis), and serumfree supplement (B27; 1x; Gibco, Grand Island, NY) in a 3:1 mix of Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) and Ham's F-12 Nutrient Mixture (F-12; Gibco). Neurospheres were mechanically triturated with a flame-drawn glass Pasteur pipette twice weekly and re-seeded in fresh culture medium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from tumor neurospheres using TRI Reagent (Molecular Research Center, Cincinnati, OH). Thereafter, RNA samples were hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array using 3' IVT express kit (Affymetrix Inc, Santa Clara, California).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labelling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix scanning protocol
| | Sample_data_processing | The data were processed using mas5 normalized scaled and log2 scaleded in R2.14
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carol,,Tang
| | Sample_contact_phone | 6564070112
| | Sample_contact_laboratory | Brain Tumor Stem cells
| | Sample_contact_department | SICS
| | Sample_contact_institute | NNI
| | Sample_contact_address | 11 Jalan Tan Tock Seng
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | Singapore
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM901nnn/GSM901004/suppl/GSM901004_AFG1133-32.CEL.gz
| | Sample_series_id | GSE36782
| | Sample_data_row_count | 54675
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