Search results for the GEO ID: GSE37208  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM913721 | GPL570 |
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HCCLM3-siRNA-Scramble
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HCCLM3, scramble siRNA control
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cell line: HCCLM3
cell line type: hepatocellular carcinoma cells
transfected sirna: scramble
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| Sample_geo_accession | GSM913721
| | Sample_status | Public on Apr 23 2012
| | Sample_submission_date | Apr 11 2012
| | Sample_last_update_date | Apr 23 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The siRNA duplexes were synthesized by Genepharma Company (Shanghai, China). Transfection of the siRNAs into HCCLM3 was done using X-tremeGENE siRNA Transfection Reagent (Roche Diagnostics GmbH, Penzberg, Germany).
| | Sample_growth_protocol_ch1 | Cells were maintained in DMEM (Dulbecco’s Modified Eagle Medium) supplemented with 10% (v/v) fetal calf serum at 37°C in a humidified incubator under 5% CO2 conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 7G.
| | Sample_data_processing | The data were analyzed with PARTEK Genomics Suite 6.4 using RMA normalization.
| | Sample_platform_id | GPL570
| | Sample_contact_name | zhigang,,zhang
| | Sample_contact_email | zzhang@shsci.org
| | Sample_contact_department | Shanghai cancer Institute
| | Sample_contact_institute | Shanghai Jiaotong University
| | Sample_contact_address | No. 800, Dong Chuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200240
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM913nnn/GSM913721/suppl/GSM913721_HCCLM3-siRNA-Scramble.CEL.gz
| | Sample_series_id | GSE37208
| | Sample_data_row_count | 54613
| | |
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GSM913722 | GPL570 |
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HCCLM3-siRNA-CTHRC1
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HCCLM3, CTHRC1 siRNA
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cell line: HCCLM3
cell line type: hepatocellular carcinoma cells
transfected sirna: CTHRC1
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| Sample_geo_accession | GSM913722
| | Sample_status | Public on Apr 23 2012
| | Sample_submission_date | Apr 11 2012
| | Sample_last_update_date | Apr 23 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The siRNA duplexes were synthesized by Genepharma Company (Shanghai, China). Transfection of the siRNAs into HCCLM3 was done using X-tremeGENE siRNA Transfection Reagent (Roche Diagnostics GmbH, Penzberg, Germany).
| | Sample_growth_protocol_ch1 | Cells were maintained in DMEM (Dulbecco’s Modified Eagle Medium) supplemented with 10% (v/v) fetal calf serum at 37°C in a humidified incubator under 5% CO2 conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (3_ivt_express_kit_manual Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 7G.
| | Sample_data_processing | The data were analyzed with PARTEK Genomics Suite 6.4 using RMA normalization.
| | Sample_platform_id | GPL570
| | Sample_contact_name | zhigang,,zhang
| | Sample_contact_email | zzhang@shsci.org
| | Sample_contact_department | Shanghai cancer Institute
| | Sample_contact_institute | Shanghai Jiaotong University
| | Sample_contact_address | No. 800, Dong Chuan Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200240
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM913nnn/GSM913722/suppl/GSM913722_HCCLM3-siRNA-CTHRC1.CEL.gz
| | Sample_series_id | GSE37208
| | Sample_data_row_count | 54613
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