Result

Search results for the GEO ID: GSE37225

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM914041

GPL1261

01_JK1(WT-)

CD11b- IgA+ cells

background strain: Balb/c gender: female age: 9 wks cell type: CD11b- IgA+ tissue: intestine

Sample_geo_accession	GSM914041
Sample_status	Public on Apr 01 2013
Sample_submission_date	Apr 12 2012
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	No treatment was applied
Sample_growth_protocol_ch1	Primary cells isolated from murine small intestine
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rneasy kit
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Isolated total RNA were amplified and labeled as described in the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA). First, total RNA (1 µg) was converted into double-stranded cDNA using the One-Cycle cDNA Synthesis Kit (Affymetrix). Double-stranded cDNA was purified by using a GeneChip Sample Cleanup Module (Affymetrix). In vitro transcription reactions were performed using a GeneChip IVT Labeling Kit, which includes T7 RNA polymerase and Biotin-labeled ribonucleotides. Biotin-labeled cRNA was purified using a GeneChip Sample Cleanup Module. The concentration of cRNA was calculated from light absorbance at 260 nm using a UV spectrophotometer.
Sample_hyb_protocol	cRNA (15 µg) was fragmented at 94°C in the presence of a fragmentation buffer (Affymetrix). Fifteen micrograms of the cRNA was hybridized to Affymetrix GeneChip® Mouse Genome 430 2.0 Array (Affymetrix).
Sample_scan_protocol	The array was incubated for 16 hr at 45°C, then automatically washed and stained with GeneChip Hybridization, Wash and Stain Kit (Affymetrix). The Probe Array was scanned using a GeneChip Scanner 3000 7G.
Sample_data_processing	The scanned data were processed with Affymetrix® Expression Console™ by MAS 5.0 algorithm .
Sample_platform_id	GPL1261
Sample_contact_name	Jun,,Kunisawa
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914041/suppl/GSM914041_01_JK1_WT-_No_863-P.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914041/suppl/GSM914041_01_JK1_WT-_No_863-P.mas5.CHP.gz
Sample_series_id	GSE37225
Sample_data_row_count	45101

GSM914042

GPL1261

02_JK2(WT+)

CD11b+ IgA+ cells

background strain: Balb/c gender: female age: 9 wks cell type: CD11b+ IgA+ tissue: intestine

Sample_geo_accession	GSM914042
Sample_status	Public on Apr 01 2013
Sample_submission_date	Apr 12 2012
Sample_last_update_date	Apr 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	No treatment was applied
Sample_growth_protocol_ch1	Primary cells isolated from murine small intestine
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Rneasy kit
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Isolated total RNA were amplified and labeled as described in the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA). First, total RNA (1 µg) was converted into double-stranded cDNA using the One-Cycle cDNA Synthesis Kit (Affymetrix). Double-stranded cDNA was purified by using a GeneChip Sample Cleanup Module (Affymetrix). In vitro transcription reactions were performed using a GeneChip IVT Labeling Kit, which includes T7 RNA polymerase and Biotin-labeled ribonucleotides. Biotin-labeled cRNA was purified using a GeneChip Sample Cleanup Module. The concentration of cRNA was calculated from light absorbance at 260 nm using a UV spectrophotometer.
Sample_hyb_protocol	cRNA (15 µg) was fragmented at 94°C in the presence of a fragmentation buffer (Affymetrix). Fifteen micrograms of the cRNA was hybridized to Affymetrix GeneChip® Mouse Genome 430 2.0 Array (Affymetrix).
Sample_scan_protocol	The array was incubated for 16 hr at 45°C, then automatically washed and stained with GeneChip Hybridization, Wash and Stain Kit (Affymetrix). The Probe Array was scanned using a GeneChip Scanner 3000 7G.
Sample_data_processing	The scanned data were processed with Affymetrix® Expression Console™ by MAS 5.0 algorithm .
Sample_platform_id	GPL1261
Sample_contact_name	Jun,,Kunisawa
Sample_contact_institute	The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914042/suppl/GSM914042_02_JK2_WT+_No_863-P.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914042/suppl/GSM914042_02_JK2_WT+_No_863-P.mas5.CHP.gz
Sample_series_id	GSE37225
Sample_data_row_count	45101

GSM914043

GPL1261

01_WT CD11b-

CD11b- IgA+ cells

background strain: Balb/c gender: female age: 9 wks cell type: CD11b- IgA+ tissue: intestine

GSM914044

GPL1261

02_WT CD11b+

CD11b+ IgA+ cells

background strain: Balb/c gender: female age: 9 wks cell type: CD11b+ IgA+ tissue: intestine

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