Search results for the GEO ID: GSE37258 ![](/q11.jpeg) |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM914974 | GPL570 |
|
KS-fs, biological rep1
|
fibroblast cells from a KS patient, rep 1
|
karyotype: 47, XXY
cell type: fibroblast
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS fibroblast cells
|
Sample_geo_accession | GSM914974
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914974/suppl/GSM914974_K-fs_P15_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914975 | GPL570 |
|
KS-fs, biological rep2
|
fibroblast cells from a KS patient, rep 2
|
karyotype: 47, XXY
cell type: fibroblast
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS fibroblast cells
|
Sample_geo_accession | GSM914975
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914975/suppl/GSM914975_K-fs_P16_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914976 | GPL570 |
|
KS-fs, biological rep3
|
fibroblast cells from a KS patient, rep 3
|
karyotype: 47, XXY
cell type: fibroblast
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS fibroblast cells
|
Sample_geo_accession | GSM914976
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914976/suppl/GSM914976_K-fs_P17_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914977 | GPL570 |
|
KS-iPSC-1, biological rep1
|
a line of iPSCs from KS fibroblast cells (KS-iPSC-1), rep1
|
karyotype: 47, XXY
cell type: iPSC
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS iPSCs
|
Sample_geo_accession | GSM914977
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914977/suppl/GSM914977_K-i-1_P23_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914978 | GPL570 |
|
KS-iPSC-1, biological rep2
|
a line of iPSCs from KS fibroblast cells (KS-iPSC-1), rep2
|
karyotype: 47, XXY
cell type: iPSC
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS iPSCs
|
Sample_geo_accession | GSM914978
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914978/suppl/GSM914978_K-i-1_P24_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914979 | GPL570 |
|
KS-iPSC-1, biological rep3
|
a line of iPSCs from KS fibroblast cells (KS-iPSC-1), rep3
|
karyotype: 47, XXY
cell type: iPSC
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS iPSCs
|
Sample_geo_accession | GSM914979
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914979/suppl/GSM914979_K-i-1_P25_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914980 | GPL570 |
|
KS-iPSC-4, biological rep1
|
the other line of iPSCs from KS fibroblast cells (KS-iPSC-4), rep1
|
karyotype: 47, XXY
cell type: iPSC
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS iPSCs
|
Sample_geo_accession | GSM914980
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914980/suppl/GSM914980_K-i-4_P22_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914981 | GPL570 |
|
KS-iPSC-4, biological rep2
|
the other line of iPSCs from KS fibroblast cells (KS-iPSC-4), rep2
|
karyotype: 47, XXY
cell type: iPSC
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS iPSCs
|
Sample_geo_accession | GSM914981
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914981/suppl/GSM914981_K-i-4_P23_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914982 | GPL570 |
|
KS-iPSC-4, biological rep3
|
the other line of iPSCs from KS fibroblast cells (KS-iPSC-4), rep3
|
karyotype: 47, XXY
cell type: iPSC
disease state: Klinefelter’s Syndrome
|
Gene Expression data of KS iPSCs
|
Sample_geo_accession | GSM914982
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914982/suppl/GSM914982_K-i-4_P24_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914983 | GPL570 |
|
N-fs, biological rep1
|
fibroblast cells from normal person, rep1
|
karyotype: 46, XY
cell type: fibroblast
disease state: control
|
Gene Expression data of normal fibroblast cells
|
Sample_geo_accession | GSM914983
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914983/suppl/GSM914983_N-fs_P14_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914984 | GPL570 |
|
N-fs, biological rep2
|
fibroblast cells from normal person, rep2
|
karyotype: 46, XY
cell type: fibroblast
disease state: control
|
Gene Expression data of normal fibroblast cells
|
Sample_geo_accession | GSM914984
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914984/suppl/GSM914984_N-fs_P15_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914985 | GPL570 |
|
N-fs, biological rep3
|
fibroblast cells from normal person, rep3
|
karyotype: 46, XY
cell type: fibroblast
disease state: control
|
Gene Expression data of normal fibroblast cells
|
Sample_geo_accession | GSM914985
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914985/suppl/GSM914985_N-fs_P16_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914986 | GPL570 |
|
N-iPSC-1, biological rep1
|
a line of iPSCs from normal fibroblast cells (N-iPSC-1), rep1
|
karyotype: 46, XY
cell type: iPSC
disease state: control
|
Gene Expression data of normal iPSCs
|
Sample_geo_accession | GSM914986
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914986/suppl/GSM914986_N-i-1_P24_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914987 | GPL570 |
|
N-iPSC-1, biological rep2
|
a line of iPSCs from normal fibroblast cells (N-iPSC-1), rep2
|
karyotype: 46, XY
cell type: iPSC
disease state: control
|
Gene Expression data of normal iPSCs
|
Sample_geo_accession | GSM914987
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914987/suppl/GSM914987_N-i-1_P25_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914988 | GPL570 |
|
N-iPSC-1, biological rep3
|
a line of iPSCs from normal fibroblast cells (N-iPSC-1), rep3
|
karyotype: 46, XY
cell type: iPSC
disease state: control
|
Gene Expression data of normal iPSCs
|
Sample_geo_accession | GSM914988
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914988/suppl/GSM914988_N-i-1_P26_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914989 | GPL570 |
|
hESCs, biological rep1
|
a line of human embryonic stem cells (SHhES2), rep1
|
karyotype: 46, XY
cell type: hESC
disease state: control
|
Gene Expression data of human embryonic stem cells
|
Sample_geo_accession | GSM914989
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914989/suppl/GSM914989_SHhES2P43_HG-U133_Plus_2_2.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914990 | GPL570 |
|
hESCs, biological rep2
|
a line of human embryonic stem cells (SHhES2), rep2
|
karyotype: 46, XY
cell type: hESC
disease state: control
|
Gene Expression data of human embryonic stem cells
|
Sample_geo_accession | GSM914990
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914990/suppl/GSM914990_SHhES2P44_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
GSM914991 | GPL570 |
|
hESCs, biological rep3
|
a line of human embryonic stem cells (SHhES2), rep3
|
karyotype: 46, XY
cell type: hESC
disease state: control
|
Gene Expression data of human embryonic stem cells
|
Sample_geo_accession | GSM914991
| Sample_status | Public on Apr 14 2012
| Sample_submission_date | Apr 13 2012
| Sample_last_update_date | Apr 14 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Normal and KS fibroblast cells were transduced by retrovirus coding hOCT4, hSOX2, hKLF4 and hC-MYC and replated the next day. In the 12th day, human embryonic stem cell-like colonies were picked and expanded on MEF in KO-DMEM medium with 20% Knockout Serum Replacement, 100 U/ml penicillin, 100 g/ml streptomycin, 0.1 mM beta-mercaptoethanol (Sigma), 2 mM L-glutamine, 1% non essential amino acid and 4 ng/ml bFGF.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted by Trizol as the manufacturer's protocol and purified by QIAGEN RNeasy kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was labelled and purified by using GeneChip 3' IVT Express Kit of Affymetrix as the manufacturer's instructions.
| Sample_hyb_protocol | Array hybridization was processed using GeneChip Hybridization, Wash and Stain Kit (Affymetrix) and Fluidics Station 450 (Affymetrix) followed the manufacturer's instructions.
| Sample_scan_protocol | Slids were scanned by GeneChip Scanner 3000 (Affymetrix) and Command Console Software 3.1 (Affymetrix) with default settings.
| Sample_data_processing | Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
| Sample_platform_id | GPL570
| Sample_contact_name | Yu,,Ma
| Sample_contact_email | nkmy13@163.com
| Sample_contact_institute | Institute of Health Sciences
| Sample_contact_address | 225 South Chongqing Road
| Sample_contact_city | Shanghai
| Sample_contact_zip/postal_code | 200025
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM914nnn/GSM914991/suppl/GSM914991_SHhES2P45_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE37258
| Sample_data_row_count | 54675
| |
|
|
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(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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