Search results for the GEO ID: GSE37290  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM915659 | GPL570 |
|
RMG2-HNF1b-sh2-rep1
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_196459)
|
Sh2_1
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915659
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915659/suppl/GSM915659_Sh2_1.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915660 | GPL570 |
|
RMG2-HNF1b-sh1-rep5
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_204881)
|
Sh1_5
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915660
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915660/suppl/GSM915660_Sh1_5.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915661 | GPL570 |
|
RMG2-HNF1b-sh2-rep2
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_196459)
|
Sh2_2
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915661
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915661/suppl/GSM915661_Sh2_2.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915662 | GPL570 |
|
RMG2-HNF1b-sh2-rep3
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_196459)
|
Sh2_3
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915662
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915662/suppl/GSM915662_Sh2_3.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915663 | GPL570 |
|
RMG2-HNF1b-sh2-rep4
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_196459)
|
Sh2_4
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915663
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915663/suppl/GSM915663_Sh2_4.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915664 | GPL570 |
|
RMG2-HNF1b-sh2-rep5
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_196459)
|
Sh2_5
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915664
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915664/suppl/GSM915664_Sh2_5.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915665 | GPL570 |
|
RMG2-HNF1b-sh1-rep1
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_204881)
|
Sh1_1
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915665
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915665/suppl/GSM915665_Sh1_1.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915666 | GPL570 |
|
RMG2-HNF1b-sh1-rep2
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_204881)
|
Sh1_2
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915666
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915666/suppl/GSM915666_Sh1_2.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915667 | GPL570 |
|
RMG2-HNF1b-sh1-rep3
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_204881)
|
Sh1_3
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915667
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915667/suppl/GSM915667_Sh1_3.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915668 | GPL570 |
|
RMG2-HNF1b-sh1-rep4
|
RMG2_HNF1b knockdown
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: HNF1b-targeting shRNA (clones V2LHS_204881)
|
Sh1_4
Gene expression data from HNF1b gene supressed cells
|
| Sample_geo_accession | GSM915668
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915668/suppl/GSM915668_Sh1_4.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915669 | GPL570 |
|
RMG2-control-rep1
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_1
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915669
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915669/suppl/GSM915669_Cont_1.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915670 | GPL570 |
|
RMG2-control-rep2
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_2
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915670
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915670/suppl/GSM915670_Cont_2.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915671 | GPL570 |
|
RMG2-control-rep3
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_3
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915671
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915671/suppl/GSM915671_Cont_3.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915672 | GPL570 |
|
RMG2-control-rep4
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_4
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915672
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915672/suppl/GSM915672_Cont_4_1.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915673 | GPL570 |
|
RMG2-control-rep5
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_5
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915673
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915673/suppl/GSM915673_Cont_5.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915674 | GPL570 |
|
RMG2-control-rep6
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_6
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915674
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915674/suppl/GSM915674_Cont_6.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915675 | GPL570 |
|
RMG2-control-rep7
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_7
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915675
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915675/suppl/GSM915675_Cont_7.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915676 | GPL570 |
|
RMG2-control-rep8
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_8
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915676
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915676/suppl/GSM915676_Cont_8.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915677 | GPL570 |
|
RMG2-control-rep9
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_9
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915677
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915677/suppl/GSM915677_Cont_9.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
GSM915678 | GPL570 |
|
RMG2-control-rep10
|
RMG2_non-silencing control
|
cell line: RMG2
cell type: Human ovarian clear cell carcinoma (OCCC) cells
transfected with: non-silencing control
|
Cont_10
Gene expression data as non-silencing control
|
| Sample_geo_accession | GSM915678
| | Sample_status | Public on May 01 2012
| | Sample_submission_date | Apr 16 2012
| | Sample_last_update_date | May 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RMG2 cells transfected with ｔwo HNF1β-targeting shRNA clones (clone IDs: V2LHS_204881 and V2LHS_196459) and a non-silencing control (clone ID: RHS4348) (Thermo Fisher Scientific, Huntsville, AL, USA), using a standard protocol with lentiviruses.
| | Sample_growth_protocol_ch1 | RMG2 cells were cultured in RPMI (Nikken, Kyoto, Japan) supplemented with 10% FBS in a humidified atmosphere containing 5% CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000 7G.
| | Sample_data_processing | Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor Affy package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | TAKAKO,,OKAMOTO
| | Sample_contact_email | okmttkko@kuhp.kyoto-u.ac.jp
| | Sample_contact_institute | Kyoto University
| | Sample_contact_address | 54 Kawahara-cho, shogoin,sakyo-ku,
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8273
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM915nnn/GSM915678/suppl/GSM915678_Cont_10.CEL.gz
| | Sample_series_id | GSE37290
| | Sample_data_row_count | 54675
| | |
|
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