Search results for the GEO ID: GSE37346  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM916525 | GPL1261 |
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wild type MEF
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embryonic fibroblasts
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strain: C57BL/6
age: embryonic day 12.5
passage no.: 1
genotype: wild type
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| Sample_geo_accession | GSM916525
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | Apr 17 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MEFs were cultured in Dulbecco’s minimal essential medium containing 10% fetal calf serum and 1% (w/v) penicillin/streptomycin on gelatin-coated dishes at 37˚C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme Mix to synthesize Biotin-Labeled cRNA.
| | Sample_hyb_protocol | Heat the Hybridization Cocktail at 99°C for 5 minutes. Cool to 45°C for 5 minutes. Inject the appropriate amount of the specific sample into the Affymetrix Mouse Genome 430 2.0 array through one of the septa. Place array in 45°C hybridization oven, at 60 rpm, and incubate for 16 hours.
| | Sample_scan_protocol | GeneChip Scaner 3000 was used . The core software was used GCOS for image processing.
| | Sample_data_processing | The summarized probe set data were generated using Expression console. Analyzing algorithm for the data was MAS5 by Affymetrix.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916525/suppl/GSM916525_WT_.CEL.gz
| | Sample_series_id | GSE37346
| | Sample_series_id | GSE38154
| | Sample_data_row_count | 45101
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GSM916526 | GPL1261 |
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Phc2 L307R MEF
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embryonic fibroblasts
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strain: C57BL/6
age: embryonic day 12.5
passage no.: 1
genotype: Phc2 L307R/L307R
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| Sample_geo_accession | GSM916526
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | Apr 17 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MEFs were cultured in Dulbecco’s minimal essential medium containing 10% fetal calf serum and 1% (w/v) penicillin/streptomycin on gelatin-coated dishes at 37˚C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme Mix to synthesize Biotin-Labeled cRNA.
| | Sample_hyb_protocol | Heat the Hybridization Cocktail at 99°C for 5 minutes. Cool to 45°C for 5 minutes. Inject the appropriate amount of the specific sample into the Affymetrix Mouse Genome 430 2.0 array through one of the septa. Place array in 45°C hybridization oven, at 60 rpm, and incubate for 16 hours.
| | Sample_scan_protocol | GeneChip Scaner 3000 was used . The core software was used GCOS for image processing.
| | Sample_data_processing | The summarized probe set data were generated using Expression console. Analyzing algorithm for the data was MAS5 by Affymetrix.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916526/suppl/GSM916526_L307RL307R.CEL.gz
| | Sample_series_id | GSE37346
| | Sample_series_id | GSE38154
| | Sample_data_row_count | 45101
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