Search results for the GEO ID: GSE37383  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM916567 | GPL1261 |
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Vehicle treated (OIL)
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Ovaries from mice treated with vehicle (oil)
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genotype: wild type
age: 24-28 d
tissue: ovary
genetic background: CD-1
treatment: oil vehicle
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Mouse, ovary, ovulation, progesterone receptor, contraception, Ulipristal acetate
Vehicle/oil treated control
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| Sample_geo_accession | GSM916567
| | Sample_status | Public on Oct 04 2012
| | Sample_submission_date | Apr 17 2012
| | Sample_last_update_date | Oct 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Microarray analyses were performed using total RNA samples prepared from vehicle or UPA-treated mice. Mice were treated with PMSG for 48 h followed by hCG. Two hour after hCG treatment, mice were administered with UPA or vehicle. Ovaries were collected at 11 h and total RNA was analyzed using Affymetrix mouse arrays (GeneChip Mouse Genome 430 2.0 Array; Affymetrix, Inc., Santa Clara, CA)
| | Sample_growth_protocol_ch1 | Mice were maintained in the designated animal care facility at University of Illinois at Urbana-Champaign according to the institutional guidelines for the care and use of laboratory animals.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from ovaries using Trizol reagent and RNA was cleaned up by the Qiagen RNAeasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The Keck Center Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign prepared amplified, biotin-labeled, fragmented antisense RNA from 100 nanograms of sample total RNA using the Affymetrix 3' Express Kit (Affymetrix, Inc., Santa Clara, CA) according to manufacturer’s instructions.
| | Sample_hyb_protocol | 10 micrograms of labeled aRNA were hybridized to a Mouse Genome 430 2.0 Affymetrix 3' Expression Analysis Array. Wash and stain were performed on Affymetrix Fluidics Station 450 with protocol EukGE_WS2v5 using Affymetrix GeneChip Command Console Fluidics Control software and solutions prepared by Keck Center,Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.
| | Sample_scan_protocol | Arrays were scanned on Affymetrix GeneChip Scanner model 3000 7G Plus using GeneChip Command Console Scan Control software to generate DAT and CEL files.
| | Sample_data_processing | To generate quality control metrics the Keck Center generated CHP files using the MAS5 algorithm with Affymetrix Expression Console version 1_1.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Shanmugasundaram,,Nallasamy
| | Sample_contact_email | snallas2@illinois.edu
| | Sample_contact_phone | 217-333-1731
| | Sample_contact_laboratory | Bagchi Lab
| | Sample_contact_department | Comparative Biosciences
| | Sample_contact_institute | University of illinois at Urbana-Champaign
| | Sample_contact_address | 2001 S Lincoln Ave
| | Sample_contact_city | Urbana
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 61802
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916567/suppl/GSM916567_OIL.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916567/suppl/GSM916567_OIL.CHP.gz
| | Sample_series_id | GSE37353
| | Sample_series_id | GSE37383
| | Sample_data_row_count | 45101
| | |
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GSM916568 | GPL1261 |
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Ulipristal/CDB treated
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Ovaries from mice treated with CDB
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genotype: wild type
age: 24-28 d
tissue: ovary
genetic background: CD-1
treatment: Ulipristal acetate
|
Mouse, ovary, ovulation, progesterone receptor, contraception, Ulipristal acetate
Ulipristal/CDB treated
|
| Sample_geo_accession | GSM916568
| | Sample_status | Public on Oct 04 2012
| | Sample_submission_date | Apr 17 2012
| | Sample_last_update_date | Oct 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Microarray analyses were performed using total RNA samples prepared from vehicle or UPA-treated mice. Mice were treated with PMSG for 48 h followed by hCG. Two hour after hCG treatment, mice were administered with UPA or vehicle. Ovaries were collected at 11 h and total RNA was analyzed using Affymetrix mouse arrays (GeneChip Mouse Genome 430 2.0 Array; Affymetrix, Inc., Santa Clara, CA)
| | Sample_growth_protocol_ch1 | Mice were maintained in the designated animal care facility at University of Illinois at Urbana-Champaign according to the institutional guidelines for the care and use of laboratory animals.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from ovaries using Trizol reagent and RNA was cleaned up by the Qiagen RNAeasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The Keck Center Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign prepared amplified, biotin-labeled, fragmented antisense RNA from 100 nanograms of sample total RNA using the Affymetrix 3' Express Kit (Affymetrix, Inc., Santa Clara, CA) according to manufacturer’s instructions.
| | Sample_hyb_protocol | 10 micrograms of labeled aRNA were hybridized to a Mouse Genome 430 2.0 Affymetrix 3' Expression Analysis Array. Wash and stain were performed on Affymetrix Fluidics Station 450 with protocol EukGE_WS2v5 using Affymetrix GeneChip Command Console Fluidics Control software and solutions prepared by Keck Center,Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.
| | Sample_scan_protocol | Arrays were scanned on Affymetrix GeneChip Scanner model 3000 7G Plus using GeneChip Command Console Scan Control software to generate DAT and CEL files.
| | Sample_data_processing | To generate quality control metrics the Keck Center generated CHP files using the MAS5 algorithm with Affymetrix Expression Console version 1_1.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Shanmugasundaram,,Nallasamy
| | Sample_contact_email | snallas2@illinois.edu
| | Sample_contact_phone | 217-333-1731
| | Sample_contact_laboratory | Bagchi Lab
| | Sample_contact_department | Comparative Biosciences
| | Sample_contact_institute | University of illinois at Urbana-Champaign
| | Sample_contact_address | 2001 S Lincoln Ave
| | Sample_contact_city | Urbana
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 61802
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916568/suppl/GSM916568_CDB.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916568/suppl/GSM916568_CDB.CHP.gz
| | Sample_series_id | GSE37353
| | Sample_series_id | GSE37383
| | Sample_data_row_count | 45101
| | |
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GSM916569 | GPL1261 |
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wild type
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Ovaries from wt mice
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age: 24-28 d
tissue: ovary
genetic background: C57BL/6
genotype: wild type
|
Mouse, ovary, ovulation, progesterone receptor, contraception, Ulipristal acetate
WT
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| Sample_geo_accession | GSM916569
| | Sample_status | Public on Oct 04 2012
| | Sample_submission_date | Apr 17 2012
| | Sample_last_update_date | Oct 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Microarray analyses were performed using total RNA samples prepared from WT or PRKO mice. Mice were treated with PMSG for 48 h followed by hCG. Ovaries were collected at 11 h and total RNA was analyzed using Affymetrix mouse arrays (GeneChip Mouse Genome 430 2.0 Array; Affymetrix, Inc., Santa Clara, CA)
| | Sample_growth_protocol_ch1 | Mice were maintained in the designated animal care facility at University of Illinois at Urbana-Champaign according to the institutional guidelines for the care and use of laboratory animals.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from ovaries using Trizol reagent and RNA was cleaned up by the Qiagen RNAeasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The Keck Center Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign prepared amplified, biotin-labeled, fragmented antisense RNA from 100 nanograms of sample total RNA using the Affymetrix 3' Express Kit (Affymetrix, Inc., Santa Clara, CA) according to manufacturer’s instructions.
| | Sample_hyb_protocol | 10 micrograms of labeled aRNA were hybridized to a Mouse Genome 430 2.0 Affymetrix 3' Expression Analysis Array. Wash and stain were performed on Affymetrix Fluidics Station 450 with protocol EukGE_WS2v5 using Affymetrix GeneChip Command Console Fluidics Control software and solutions prepared by Keck Center,Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.
| | Sample_scan_protocol | Arrays were scanned on Affymetrix GeneChip Scanner model 3000 7G Plus using GeneChip Command Console Scan Control software to generate DAT and CEL files.
| | Sample_data_processing | To generate quality control metrics the Keck Center generated CHP files using the MAS5 algorithm with Affymetrix Expression Console version 1_1.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Shanmugasundaram,,Nallasamy
| | Sample_contact_email | snallas2@illinois.edu
| | Sample_contact_phone | 217-333-1731
| | Sample_contact_laboratory | Bagchi Lab
| | Sample_contact_department | Comparative Biosciences
| | Sample_contact_institute | University of illinois at Urbana-Champaign
| | Sample_contact_address | 2001 S Lincoln Ave
| | Sample_contact_city | Urbana
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 61802
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916569/suppl/GSM916569_WT.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916569/suppl/GSM916569_WT.CHP.gz
| | Sample_series_id | GSE37354
| | Sample_series_id | GSE37383
| | Sample_data_row_count | 45101
| | |
|
GSM916570 | GPL1261 |
|
PR knockout
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Ovaries from PRKO mice
|
age: 24-28 d
tissue: ovary
genetic background: C57BL/6
genotype: PR knockout
|
Mouse, ovary, ovulation, progesterone receptor, contraception, Ulipristal acetate
PRKO
|
| Sample_geo_accession | GSM916570
| | Sample_status | Public on Oct 04 2012
| | Sample_submission_date | Apr 17 2012
| | Sample_last_update_date | Oct 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Microarray analyses were performed using total RNA samples prepared from WT or PRKO mice. Mice were treated with PMSG for 48 h followed by hCG. Ovaries were collected at 11 h and total RNA was analyzed using Affymetrix mouse arrays (GeneChip Mouse Genome 430 2.0 Array; Affymetrix, Inc., Santa Clara, CA)
| | Sample_growth_protocol_ch1 | Mice were maintained in the designated animal care facility at University of Illinois at Urbana-Champaign according to the institutional guidelines for the care and use of laboratory animals.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from ovaries using Trizol reagent and RNA was cleaned up by the Qiagen RNAeasy kit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The Keck Center Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign prepared amplified, biotin-labeled, fragmented antisense RNA from 100 nanograms of sample total RNA using the Affymetrix 3' Express Kit (Affymetrix, Inc., Santa Clara, CA) according to manufacturer’s instructions.
| | Sample_hyb_protocol | 10 micrograms of labeled aRNA were hybridized to a Mouse Genome 430 2.0 Affymetrix 3' Expression Analysis Array. Wash and stain were performed on Affymetrix Fluidics Station 450 with protocol EukGE_WS2v5 using Affymetrix GeneChip Command Console Fluidics Control software and solutions prepared by Keck Center,Comparative and Functional Genomics Unit, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.
| | Sample_scan_protocol | Arrays were scanned on Affymetrix GeneChip Scanner model 3000 7G Plus using GeneChip Command Console Scan Control software to generate DAT and CEL files.
| | Sample_data_processing | To generate quality control metrics the Keck Center generated CHP files using the MAS5 algorithm with Affymetrix Expression Console version 1_1.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Shanmugasundaram,,Nallasamy
| | Sample_contact_email | snallas2@illinois.edu
| | Sample_contact_phone | 217-333-1731
| | Sample_contact_laboratory | Bagchi Lab
| | Sample_contact_department | Comparative Biosciences
| | Sample_contact_institute | University of illinois at Urbana-Champaign
| | Sample_contact_address | 2001 S Lincoln Ave
| | Sample_contact_city | Urbana
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 61802
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916570/suppl/GSM916570_PRKO.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM916nnn/GSM916570/suppl/GSM916570_PRKO.CHP.gz
| | Sample_series_id | GSE37354
| | Sample_series_id | GSE37383
| | Sample_data_row_count | 45101
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