Result

Search results for the GEO ID: GSE37397

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Title

Source name

Description

Characteristics

GSM917607

GPL1261

mouse embryonic fibroblasts

strain: C57BL6 cell type: embryonic fibroblasts gender: male tissue: embryo

primary cell

Sample_geo_accession	GSM917607
Sample_status	Public on Oct 01 2012
Sample_submission_date	Apr 18 2012
Sample_last_update_date	Oct 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MEF cells were plated at 80% conﬂuence and transfected with PMX-based retroviral vectors (Addgene, Cambridge, MA, USA) containing murine Oct4, Sox2, and Klf4 cDNAs using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions. The virus supernatant was harvested 48 hours after transfection. MEFs at passage 2 or 3 were seeded at a density of 3500–5000 cells/cm2 and incubated with filtered viral supernatants containing equal parts of the three transcription factors (Oct4, Sox2, Klf4)[88] and 5 μg/ml polybrene. Twelve hours later, the medium was gradually replaced with ESC medium supplemented with 50 μg/ml Vc (Sigma-Aldrich, St. Louis, MO, USA) using a 4-day stepwise process
Sample_growth_protocol_ch1	MEFs were derived from embryonic day 13.5 mice embryos and maintained in Dulbecco’s modified Eagle’s medium (DMEM; high glucose) containing 10% fetal bovine serum (FBS), nonessential amino acids (NEAA), L-glutamate, sodium pyruvate, and penicillin/streptomycin. ESCs and iPSCs were cultured on γ-irradiated MEFs in ES medium containing Knockout DMEM supplemented with 20% KnockOut Serum Replacement, NEAA, L-glutamate, sodium pyruvate, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, and 1000 U/ml leukemia inhibitory factor (Millipore, Billerica, MA, USA). All other reagents were purchased from Invitrogen Corp. (Carlsbad, CA, USA).
Sample_growth_protocol_ch1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified using TRIzol reagent (Invitrogen) and reverse transcribed using a reverse transcription system (Promega, Madison, WI, USA) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-cycle target labeling protocol was completed with the reagent MessageAmp II-Biotin (Ambion, #1791) and Poly-A RNA Control Kit (Affymetrix, P/N 900433),
Sample_hyb_protocol	Hybridization Control Kit (Affymetrix, P/N 900454). Hybridization Oven 640. Affymetrix Gene Chip Mouse Genome 430 2.0 Array (GEO accession number: platform GPL1261, Affymetrix, Santa Clara, CA, USA) was used for microarray hybridizations to examine global gene expression of samples at Capital biochip Corp. (Beijing, China) in which the gene chip microarray service was certified by Affymetrix.
Sample_scan_protocol	GeneChip® Scanner 3000
Sample_data_processing	The normalization method in our data processing is according to the RMA (based on log (PM/MM)) with s series of calculation with some default set supplied by Affymetrix company.
Sample_platform_id	GPL1261
Sample_contact_name	zhenwei,,song
Sample_contact_email	gactu@163.com
Sample_contact_institute	sun yat-sen university
Sample_contact_address	beisan road
Sample_contact_city	guangzhou
Sample_contact_zip/postal_code	510006
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM917nnn/GSM917607/suppl/GSM917607.CEL.gz
Sample_series_id	GSE37397
Sample_data_row_count	45037

GSM917608

GPL1261

Induced pluripotent stem cells

strain: C57BL6 cell type: Induced pluripotent stem cells gender: male tissue: embryo

primary cell

Sample_geo_accession	GSM917608
Sample_status	Public on Oct 01 2012
Sample_submission_date	Apr 18 2012
Sample_last_update_date	Oct 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MEF cells were plated at 80% conﬂuence and transfected with PMX-based retroviral vectors (Addgene, Cambridge, MA, USA) containing murine Oct4, Sox2, and Klf4 cDNAs using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions. The virus supernatant was harvested 48 hours after transfection. MEFs at passage 2 or 3 were seeded at a density of 3500–5000 cells/cm2 and incubated with filtered viral supernatants containing equal parts of the three transcription factors (Oct4, Sox2, Klf4)[88] and 5 μg/ml polybrene. Twelve hours later, the medium was gradually replaced with ESC medium supplemented with 50 μg/ml Vc (Sigma-Aldrich, St. Louis, MO, USA) using a 4-day stepwise process
Sample_growth_protocol_ch1	MEFs were derived from embryonic day 13.5 mice embryos and maintained in Dulbecco’s modified Eagle’s medium (DMEM; high glucose) containing 10% fetal bovine serum (FBS), nonessential amino acids (NEAA), L-glutamate, sodium pyruvate, and penicillin/streptomycin. ESCs and iPSCs were cultured on γ-irradiated MEFs in ES medium containing Knockout DMEM supplemented with 20% KnockOut Serum Replacement, NEAA, L-glutamate, sodium pyruvate, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, and 1000 U/ml leukemia inhibitory factor (Millipore, Billerica, MA, USA). All other reagents were purchased from Invitrogen Corp. (Carlsbad, CA, USA).
Sample_growth_protocol_ch1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified using TRIzol reagent (Invitrogen) and reverse transcribed using a reverse transcription system (Promega, Madison, WI, USA) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-cycle target labeling protocol was completed with the reagent MessageAmp II-Biotin (Ambion, #1791) and Poly-A RNA Control Kit (Affymetrix, P/N 900433),
Sample_hyb_protocol	Hybridization Control Kit (Affymetrix, P/N 900454). Hybridization Oven 640. Affymetrix Gene Chip Mouse Genome 430 2.0 Array (GEO accession number: platform GPL1261, Affymetrix, Santa Clara, CA, USA) was used for microarray hybridizations to examine global gene expression of samples at Capital biochip Corp. (Beijing, China) in which the gene chip microarray service was certified by Affymetrix.
Sample_scan_protocol	GeneChip® Scanner 3000
Sample_data_processing	The normalization method in our data processing is according to the RMA (based on log (PM/MM)) with s series of calculation with some default set supplied by Affymetrix company.
Sample_platform_id	GPL1261
Sample_contact_name	zhenwei,,song
Sample_contact_email	gactu@163.com
Sample_contact_institute	sun yat-sen university
Sample_contact_address	beisan road
Sample_contact_city	guangzhou
Sample_contact_zip/postal_code	510006
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM917nnn/GSM917608/suppl/GSM917608.CEL.gz
Sample_series_id	GSE37397
Sample_data_row_count	45037

GSM917609

GPL1261

mouse embryonic fibroblasts day7

mouse embryonic fibroblasts_ ay7

strain: C57BL6 cell type: embryonic fibroblasts day7 gender: male tissue: embryo

artificial cell induced by virus

Sample_geo_accession	GSM917609
Sample_status	Public on Oct 01 2012
Sample_submission_date	Apr 18 2012
Sample_last_update_date	Oct 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MEF cells were plated at 80% conﬂuence and transfected with PMX-based retroviral vectors (Addgene, Cambridge, MA, USA) containing murine Oct4, Sox2, and Klf4 cDNAs using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions. The virus supernatant was harvested 48 hours after transfection. MEFs at passage 2 or 3 were seeded at a density of 3500–5000 cells/cm2 and incubated with filtered viral supernatants containing equal parts of the three transcription factors (Oct4, Sox2, Klf4)[88] and 5 μg/ml polybrene. Twelve hours later, the medium was gradually replaced with ESC medium supplemented with 50 μg/ml Vc (Sigma-Aldrich, St. Louis, MO, USA) using a 4-day stepwise process
Sample_growth_protocol_ch1	MEFs were derived from embryonic day 13.5 mice embryos and maintained in Dulbecco’s modified Eagle’s medium (DMEM; high glucose) containing 10% fetal bovine serum (FBS), nonessential amino acids (NEAA), L-glutamate, sodium pyruvate, and penicillin/streptomycin. ESCs and iPSCs were cultured on γ-irradiated MEFs in ES medium containing Knockout DMEM supplemented with 20% KnockOut Serum Replacement, NEAA, L-glutamate, sodium pyruvate, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, and 1000 U/ml leukemia inhibitory factor (Millipore, Billerica, MA, USA). All other reagents were purchased from Invitrogen Corp. (Carlsbad, CA, USA).
Sample_growth_protocol_ch1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified using TRIzol reagent (Invitrogen) and reverse transcribed using a reverse transcription system (Promega, Madison, WI, USA) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-cycle target labeling protocol was completed with the reagent MessageAmp II-Biotin (Ambion, #1791) and Poly-A RNA Control Kit (Affymetrix, P/N 900433),
Sample_hyb_protocol	Hybridization Control Kit (Affymetrix, P/N 900454). Hybridization Oven 640. Affymetrix Gene Chip Mouse Genome 430 2.0 Array (GEO accession number: platform GPL1261, Affymetrix, Santa Clara, CA, USA) was used for microarray hybridizations to examine global gene expression of samples at Capital biochip Corp. (Beijing, China) in which the gene chip microarray service was certified by Affymetrix.
Sample_scan_protocol	GeneChip® Scanner 3000
Sample_data_processing	The normalization method in our data processing is according to the RMA (based on log (PM/MM)) with s series of calculation with some default set supplied by Affymetrix company.
Sample_platform_id	GPL1261
Sample_contact_name	zhenwei,,song
Sample_contact_email	gactu@163.com
Sample_contact_institute	sun yat-sen university
Sample_contact_address	beisan road
Sample_contact_city	guangzhou
Sample_contact_zip/postal_code	510006
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM917nnn/GSM917609/suppl/GSM917609.CEL.gz
Sample_series_id	GSE37397
Sample_data_row_count	45037

GSM917610

GPL1261

embryonic stem cells

strain: C57BL6 cell type: embryonic stem cells gender: male tissue: embryo

primary cell

Sample_geo_accession	GSM917610
Sample_status	Public on Oct 01 2012
Sample_submission_date	Apr 18 2012
Sample_last_update_date	Oct 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	MEF cells were plated at 80% conﬂuence and transfected with PMX-based retroviral vectors (Addgene, Cambridge, MA, USA) containing murine Oct4, Sox2, and Klf4 cDNAs using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions. The virus supernatant was harvested 48 hours after transfection. MEFs at passage 2 or 3 were seeded at a density of 3500–5000 cells/cm2 and incubated with filtered viral supernatants containing equal parts of the three transcription factors (Oct4, Sox2, Klf4)[88] and 5 μg/ml polybrene. Twelve hours later, the medium was gradually replaced with ESC medium supplemented with 50 μg/ml Vc (Sigma-Aldrich, St. Louis, MO, USA) using a 4-day stepwise process
Sample_growth_protocol_ch1	MEFs were derived from embryonic day 13.5 mice embryos and maintained in Dulbecco’s modified Eagle’s medium (DMEM; high glucose) containing 10% fetal bovine serum (FBS), nonessential amino acids (NEAA), L-glutamate, sodium pyruvate, and penicillin/streptomycin. ESCs and iPSCs were cultured on γ-irradiated MEFs in ES medium containing Knockout DMEM supplemented with 20% KnockOut Serum Replacement, NEAA, L-glutamate, sodium pyruvate, 0.1 mM β-mercaptoethanol, penicillin/streptomycin, and 1000 U/ml leukemia inhibitory factor (Millipore, Billerica, MA, USA). All other reagents were purchased from Invitrogen Corp. (Carlsbad, CA, USA).
Sample_growth_protocol_ch1
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified using TRIzol reagent (Invitrogen) and reverse transcribed using a reverse transcription system (Promega, Madison, WI, USA) according to the manufacturer’s protocol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-cycle target labeling protocol was completed with the reagent MessageAmp II-Biotin (Ambion, #1791) and Poly-A RNA Control Kit (Affymetrix, P/N 900433),
Sample_hyb_protocol	Hybridization Control Kit (Affymetrix, P/N 900454). Hybridization Oven 640. Affymetrix Gene Chip Mouse Genome 430 2.0 Array (GEO accession number: platform GPL1261, Affymetrix, Santa Clara, CA, USA) was used for microarray hybridizations to examine global gene expression of samples at Capital biochip Corp. (Beijing, China) in which the gene chip microarray service was certified by Affymetrix.
Sample_scan_protocol	GeneChip® Scanner 3000
Sample_data_processing	The normalization method in our data processing is according to the RMA (based on log (PM/MM)) with s series of calculation with some default set supplied by Affymetrix company.
Sample_platform_id	GPL1261
Sample_contact_name	zhenwei,,song
Sample_contact_email	gactu@163.com
Sample_contact_institute	sun yat-sen university
Sample_contact_address	beisan road
Sample_contact_city	guangzhou
Sample_contact_zip/postal_code	510006
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM917nnn/GSM917610/suppl/GSM917610.CEL.gz
Sample_series_id	GSE37397
Sample_data_row_count	45037

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