Search results for the GEO ID: GSE37427  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM918962 | GPL570 |
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fibroblast-like synoviocytes transfected with mimic-NC
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FLS transfected with mimic-NC
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cell origin: rheumatoid arthritis (RA) patients
cell type: fibroblast-like synoviocytes
transfected with: mimic-NC
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FLS-NC_NS
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| Sample_geo_accession | GSM918962
| | Sample_status | Public on May 15 2012
| | Sample_submission_date | Apr 19 2012
| | Sample_last_update_date | May 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | fibroblast-like synoviocytes were transfected with mimic-NC or mimic-miR-23b by Lipofactamin 2000 for two times.
| | Sample_growth_protocol_ch1 | fibroblast-like synoviocytes were maintained in DME supplemented with 10% (vol/vol) FBS (Hyclone), 100 µg/ml penicillin G, and 100 µg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nan,,Shen
| | Sample_contact_email | nanshensibs@gmail.com
| | Sample_contact_department | Department of Rheumatology
| | Sample_contact_institute | Renji Hospital, JiaoTong University School of Medicine
| | Sample_contact_address | 145 Shan Dong Middle Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200001
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM918nnn/GSM918962/suppl/GSM918962_FLS-NC.CEL.gz
| | Sample_series_id | GSE37276
| | Sample_series_id | GSE37427
| | Sample_data_row_count | 54613
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GSM918963 | GPL570 |
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fibroblast-like synoviocytes transfected with mimic-miR-23b
|
FLS transfected with mimic-miR-23b
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cell origin: rheumatoid arthritis (RA) patients
cell type: fibroblast-like synoviocytes
transfected with: mimic-miR-23b
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FLS-miR-23b_NS
|
| Sample_geo_accession | GSM918963
| | Sample_status | Public on May 15 2012
| | Sample_submission_date | Apr 19 2012
| | Sample_last_update_date | May 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | fibroblast-like synoviocytes were transfected with mimic-NC or mimic-miR-23b by Lipofactamin 2000 for two times.
| | Sample_growth_protocol_ch1 | fibroblast-like synoviocytes were maintained in DME supplemented with 10% (vol/vol) FBS (Hyclone), 100 µg/ml penicillin G, and 100 µg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nan,,Shen
| | Sample_contact_email | nanshensibs@gmail.com
| | Sample_contact_department | Department of Rheumatology
| | Sample_contact_institute | Renji Hospital, JiaoTong University School of Medicine
| | Sample_contact_address | 145 Shan Dong Middle Road
| | Sample_contact_city | Shanghai
| | Sample_contact_zip/postal_code | 200001
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM918nnn/GSM918963/suppl/GSM918963_FLS-miR-23b.CEL.gz
| | Sample_series_id | GSE37276
| | Sample_series_id | GSE37427
| | Sample_data_row_count | 54613
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