Search results for the GEO ID: GSE37485  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM921274 | GPL3921 |
|
X122L.p2AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
actively proliferating pre-stasis HMEC
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
±oxytocin: +oxytocin
|
X122L.p2AM87ACTX
|
| Sample_geo_accession | GSM921274
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921274/suppl/GSM921274_5500014051302032209600_HTA37_2_Image.H05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921275 | GPL3921 |
|
X122L.p2BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
actively proliferating pre-stasis HMEC
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
±oxytocin: +oxytocin
|
X122L.p2BM87ACTX
|
| Sample_geo_accession | GSM921275
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921275/suppl/GSM921275_5500014051302032209600_HTA37_2_Image.A07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921276 | GPL3921 |
|
X122L.p4M87ACTX_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 4
±oxytocin: +oxytocin
|
X122L.p4M87ACTX
|
| Sample_geo_accession | GSM921276
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921276/suppl/GSM921276_5500014051302032209600_HTA37_2_Image.B07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921277 | GPL3921 |
|
X122L.p6AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
intermediate proliferating pre-stasis HMEC
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 6
±oxytocin: +oxytocin
|
X122L.p6AM87ACTX
|
| Sample_geo_accession | GSM921277
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921277/suppl/GSM921277_5500014051302032209600_HTA37_2_Image.C07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921278 | GPL3921 |
|
X122L.p6BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
intermediate proliferating pre-stasis HMEC
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 6
±oxytocin: +oxytocin
|
X122L.p6BM87ACTX
|
| Sample_geo_accession | GSM921278
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921278/suppl/GSM921278_5500014051302032209600_HTA37_2_Image.D07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921279 | GPL3921 |
|
X122L.p9AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 9
±oxytocin: +oxytocin
|
X122L.p9AM87ACTX
|
| Sample_geo_accession | GSM921279
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921279/suppl/GSM921279_5500014051302032209600_HTA37_2_Image.E07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921280 | GPL3921 |
|
X122L.p9BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 66
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 9
±oxytocin: +oxytocin
|
X122L.p9BM87ACTX
|
| Sample_geo_accession | GSM921280
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921280/suppl/GSM921280_5500014051302032209600_HTA37_2_Image.F07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921281 | GPL3921 |
|
X153L.p11AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 11
±oxytocin: +oxytocin
|
X153L.p11AM87ACTX
|
| Sample_geo_accession | GSM921281
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921281/suppl/GSM921281_5500014051302032209600_HTA37_2_Image.F09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921282 | GPL3921 |
|
X153L.p11BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 11
±oxytocin: +oxytocin
|
X153L.p11BM87ACTX
|
| Sample_geo_accession | GSM921282
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921282/suppl/GSM921282_5500014051302032209600_HTA37_2_Image.G09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921283 | GPL3921 |
|
X153L.p2M87ACTX_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
±oxytocin: +oxytocin
|
X153L.p2M87ACTX
|
| Sample_geo_accession | GSM921283
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921283/suppl/GSM921283_5500014051302032209600_HTA37_2_Image.G07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921284 | GPL3921 |
|
X153L.p3AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
actively proliferating pre-stasis HMEC
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 3
±oxytocin: +oxytocin
|
X153L.p3AM87ACTX
|
| Sample_geo_accession | GSM921284
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921284/suppl/GSM921284_5500014051302032209600_HTA37_2_Image.H07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921285 | GPL3921 |
|
X153L.p3BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
actively proliferating pre-stasis HMEC
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 3
±oxytocin: +oxytocin
|
X153L.p3BM87ACTX
|
| Sample_geo_accession | GSM921285
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921285/suppl/GSM921285_5500014051302032209600_HTA37_2_Image.A09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921286 | GPL3921 |
|
X153L.p5AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
intermediate proliferating pre-stasis HMEC
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X153L.p5AM87ACTX
|
| Sample_geo_accession | GSM921286
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921286/suppl/GSM921286_5500014051302032209600_HTA37_2_Image.B09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921287 | GPL3921 |
|
X153L.p5BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
intermediate proliferating pre-stasis HMEC
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X153L.p5BM87ACTX
|
| Sample_geo_accession | GSM921287
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921287/suppl/GSM921287_5500014051302032209600_HTA37_2_Image.C09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921288 | GPL3921 |
|
X153L.p8AM87ACTX_Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 8
±oxytocin: +oxytocin
|
X153L.p8AM87ACTX
|
| Sample_geo_accession | GSM921288
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921288/suppl/GSM921288_5500014051302032209600_HTA37_2_Image.D09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921289 | GPL3921 |
|
X153L.p8BM87ACTX_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 60
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 8
±oxytocin: +oxytocin
|
X153L.p8BM87ACTX
|
| Sample_geo_accession | GSM921289
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921289/suppl/GSM921289_5500014051302032209600_HTA37_2_Image.E09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921290 | GPL3921 |
|
X184D11pM85X_Pre-stasis HMEC
|
intermediate proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 11
±oxytocin: +oxytocin
|
X184D11pM85X
|
| Sample_geo_accession | GSM921290
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921290/suppl/GSM921290_5500024017800112906300.B05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921291 | GPL3921 |
|
X184D14pM85X.1_Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 14
±oxytocin: +oxytocin
|
X184D14pM85X.1
|
| Sample_geo_accession | GSM921291
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921291/suppl/GSM921291_5500024017800112906300.C05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921292 | GPL3921 |
|
X184D14pM85X.2_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 14
±oxytocin: +oxytocin
|
X184D14pM85X.2
|
| Sample_geo_accession | GSM921292
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921292/suppl/GSM921292_5500024017800112906300.D05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921293 | GPL3921 |
|
X184D2p_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
|
X184D2p
|
| Sample_geo_accession | GSM921293
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921293/suppl/GSM921293_5500024017800112906300.A04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921294 | GPL3921 |
|
X184D2pM85X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
±oxytocin: +oxytocin
|
X184D2pM85X
|
| Sample_geo_accession | GSM921294
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921294/suppl/GSM921294_5500024017800112906300.F04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921295 | GPL3921 |
|
X184D4p_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 4
|
X184D4p
|
| Sample_geo_accession | GSM921295
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921295/suppl/GSM921295_5500024017800112906300.B04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921296 | GPL3921 |
|
X184D4pM85X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 4
±oxytocin: +oxytocin
|
X184D4pM85X
|
| Sample_geo_accession | GSM921296
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921296/suppl/GSM921296_5500024017800112906300.H04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921297 | GPL3921 |
|
X184D6p.1_Pre-stasis HMEC
|
intermediate proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 6
|
X184D6p.1
|
| Sample_geo_accession | GSM921297
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921297/suppl/GSM921297_5500024017800112906300.C04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921298 | GPL3921 |
|
X184D6X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 6
±oxytocin: +oxytocin
|
X184D6X
|
| Sample_geo_accession | GSM921298
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921298/suppl/GSM921298_5500014035520022508537HTA342.E09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921299 | GPL3921 |
|
X184D9pM85.1_Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 9
|
X184D9pM85.1
|
| Sample_geo_accession | GSM921299
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921299/suppl/GSM921299_5500024017800112906300.D04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921300 | GPL3921 |
|
X184D9pM85.2_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 21
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 9
|
X184D9pM85.2
|
| Sample_geo_accession | GSM921300
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921300/suppl/GSM921300_5500024017800112906300.E04.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921301 | GPL3921 |
|
X240L10X_Pre-stasis HMEC
|
pre-stasis HMEC at stasis
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 10
±oxytocin: +oxytocin
|
X240L10X
|
| Sample_geo_accession | GSM921301
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921301/suppl/GSM921301_5500014026072032607010.B09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921302 | GPL3921 |
|
X240L11X_Pre-stasis HMEC
|
pre-stasis HMEC at stasis
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 11
±oxytocin: +oxytocin
|
X240L11X
|
| Sample_geo_accession | GSM921302
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921302/suppl/GSM921302_5500014026072032607010.C09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921303 | GPL3921 |
|
X240L2X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
±oxytocin: +oxytocin
|
X240L2X
|
| Sample_geo_accession | GSM921303
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921303/suppl/GSM921303_5500014026072032607010.G07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921304 | GPL3921 |
|
X240L5X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X240L5X
|
| Sample_geo_accession | GSM921304
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921304/suppl/GSM921304_5500014026072032607010.H07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921305 | GPL3921 |
|
X240L8X_Pre-stasis HMEC
|
intermediate proliferating pre-stasis HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 8
±oxytocin: +oxytocin
|
X240L8X
|
| Sample_geo_accession | GSM921305
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921305/suppl/GSM921305_5500014026072032607010.A09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921306 | GPL3921 |
|
X240LB_CD10.5.1_Pre-stasis myoepithelial HMEC Biological replicate #1
|
actively proliferating pre-stasis myoepithelial HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X240LB_CD10.5.1
|
| Sample_geo_accession | GSM921306
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921306/suppl/GSM921306_5500014060591072109530_HTA40_2_Image.G07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921307 | GPL3921 |
|
X240LB_CD10.5.2_Pre-stasis myoepithelial HMEC Biological replicate #1
|
actively proliferating pre-stasis myoepithelial HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X240LB_CD10.5.2
|
| Sample_geo_accession | GSM921307
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921307/suppl/GSM921307_5500014060591072109530_HTA40_2_Image.H07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921308 | GPL3921 |
|
X240LB_EpCam.5.1_Pre-stasis luminal HMEC Biological replicate #1
|
actively proliferating pre-stasis luminal HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X240LB_EpCam.5.1
|
| Sample_geo_accession | GSM921308
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921308/suppl/GSM921308_5500014060591072109530_HTA40_2_Image.A09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921309 | GPL3921 |
|
X240LB_EpCam.5.2_Pre-stasis luminal HMEC Biological replicate #1
|
actively proliferating pre-stasis luminal HMEC
|
age: 19
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X240LB_EpCam.5.2
|
| Sample_geo_accession | GSM921309
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921309/suppl/GSM921309_5500014060591072109530_HTA40_2_Image.B09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921310 | GPL3921 |
|
X250MK3pM85X.1_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 36
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 3
±oxytocin: +oxytocin
|
X250MK3pM85X.1
|
| Sample_geo_accession | GSM921310
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921310/suppl/GSM921310_5500014035520022508537HTA342.D07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921311 | GPL3921 |
|
X250MK4pM85X.1_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 36
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 4
±oxytocin: +oxytocin
|
X250MK4pM85X.1
|
| Sample_geo_accession | GSM921311
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921311/suppl/GSM921311_5500014035520022508537HTA342.E07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921312 | GPL3921 |
|
X48RT10X_Pre-stasis HMEC
|
intermediate proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 10
±oxytocin: +oxytocin
|
X48RT10X
|
| Sample_geo_accession | GSM921312
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921312/suppl/GSM921312_5500014026072032607010.D07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921313 | GPL3921 |
|
X48RT12pM85.1_Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 12
|
X48RT12pM85.1
|
| Sample_geo_accession | GSM921313
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921313/suppl/GSM921313_5500024017800112906300.H06.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921314 | GPL3921 |
|
X48RT12pM85.2_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 12
|
X48RT12pM85.2
|
| Sample_geo_accession | GSM921314
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921314/suppl/GSM921314_5500024017800112906300.A07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921315 | GPL3921 |
|
X48RT12X_Pre-stasis HMEC
|
intermediate proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 12
±oxytocin: +oxytocin
|
X48RT12X
|
| Sample_geo_accession | GSM921315
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921315/suppl/GSM921315_5500014026072032607010.E07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921316 | GPL3921 |
|
X48RT15X_Pre-stasis HMEC
|
pre-stasis HMEC at stasis
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 15
±oxytocin: +oxytocin
|
X48RT15X
|
| Sample_geo_accession | GSM921316
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921316/suppl/GSM921316_5500014026072032607010.F07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921317 | GPL3921 |
|
X48RT16X_Pre-stasis HMEC
|
pre-stasis HMEC at stasis
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 16
±oxytocin: +oxytocin
|
X48RT16X
|
| Sample_geo_accession | GSM921317
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921317/suppl/GSM921317_5500014035520022508537HTA342.C09.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921318 | GPL3921 |
|
X48RT2pM85_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
|
X48RT2pM85
|
| Sample_geo_accession | GSM921318
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921318/suppl/GSM921318_5500024017800112906300.E06.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921319 | GPL3921 |
|
X48RT3X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 3
±oxytocin: +oxytocin
|
X48RT3X
|
| Sample_geo_accession | GSM921319
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921319/suppl/GSM921319_5500014026072032607010.A07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921320 | GPL3921 |
|
X48RT4pM85_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 4
|
X48RT4pM85
|
| Sample_geo_accession | GSM921320
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921320/suppl/GSM921320_5500024017800112906300.F06.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921321 | GPL3921 |
|
X48RT5X_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 5
±oxytocin: +oxytocin
|
X48RT5X
|
| Sample_geo_accession | GSM921321
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921321/suppl/GSM921321_5500014026072032607010.B07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921322 | GPL3921 |
|
X48RT8X_Pre-stasis HMEC
|
intermediate proliferating pre-stasis HMEC
|
age: 16
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: intermediate proliferating pre-stasis HMEC
passage number: 8
±oxytocin: +oxytocin
|
X48RT8X
|
| Sample_geo_accession | GSM921322
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921322/suppl/GSM921322_5500014026072032607010.C07.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921323 | GPL3921 |
|
X96R.p2.M87ACTX_Pre-stasis HMEC
|
actively proliferating pre-stasis HMEC
|
age: 62
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: actively proliferating pre-stasis HMEC
passage number: 2
±oxytocin: +oxytocin
|
X96R.p2.M87ACTX
|
| Sample_geo_accession | GSM921323
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921323/suppl/GSM921323_5500014051302032209600_HTA37_2_Image.C05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921324 | GPL3921 |
|
X96R.p5A.M87ACTX._Pre-stasis HMEC Biological replicate #1
|
pre-stasis HMEC at stasis
|
age: 62
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 5
±oxytocin: +oxytocin
|
X96R.p5A.M87ACTX.
|
| Sample_geo_accession | GSM921324
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921324/suppl/GSM921324_5500014051302032209600_HTA37_2_Image.F05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
GSM921325 | GPL3921 |
|
X96R.p5B.M87ACTX_Pre-stasis HMEC Biological replicate #2
|
pre-stasis HMEC at stasis
|
age: 62
tissue source: human mammary gland (reduction mammoplasty tissue)
cell type: pre-stasis HMEC at stasis
passage number: 5
±oxytocin: +oxytocin
|
X96R.p5B.M87ACTX
|
| Sample_geo_accession | GSM921325
| | Sample_status | Public on May 03 2012
| | Sample_submission_date | Apr 24 2012
| | Sample_last_update_date | May 04 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell cultures were harvested and different points in their growth from early passage good growing to near stasis.
| | Sample_growth_protocol_ch1 | Finite life span HMEC were obtained from reduction mammoplasty tissue of women of the indicated ages. HMEC were grown starting from organoids in the serum-containing M85+CT±X or M87A+CT+X medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cell were lysed with trizol reagent (Invitrogen) according manufacturer's protocol. RNA was resuspended in 100 µl nuclease free water and subject to a second step purification on ion exchange column (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | For each sample, the RNA target is prepared by annealing 2.0µg of total RNA in 5µl water with 5µl of 10µM T7 (Oligo dt) primer. First and second strand cDNA synthesis is performed and the cDNA products purified using magnetic beads. Labeled cRNA is synthesized from the cDNA using T7 RNA polymerase followed by clean-up with magnetic beads. Purified cRNA is quantitated and fragmented.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT_HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Robust multi-array analysis (RMA, from affy package version 1.26.1) was performed to normalize data collected from different samples through R bioconductor (R version 2.11.1). Default parameter values were used.
| | Sample_platform_id | GPL3921
| | Sample_contact_name | Francois,,Pepin
| | Sample_contact_institute | McGill University
| | Sample_contact_address | 1160 Pine Av W
| | Sample_contact_city | Montreal
| | Sample_contact_state | Quebec
| | Sample_contact_zip/postal_code | H3A1A3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM921nnn/GSM921325/suppl/GSM921325_5500014051302032209600_HTA37_2_Image.G05.CEL.gz
| | Sample_series_id | GSE37485
| | Sample_data_row_count | 22277
| | |
|
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