Result

Search results for the GEO ID: GSE37516

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM920910

GPL1261

Control aOBSC rep1

Ctr rep1

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 1 day

replicate 1

Sample_geo_accession	GSM920910
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920910/suppl/GSM920910.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920911

GPL1261

Control aOBSC rep2

Ctr rep2

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 1 day

replicate 2

Sample_geo_accession	GSM920911
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920911/suppl/GSM920911.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920912

GPL1261

Control aOBSC rep3

Ctr rep3

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 1 day

replicate 3

Sample_geo_accession	GSM920912
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920912/suppl/GSM920912.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920913

GPL1261

C2 aOBSC rep1

C2 rep1

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 2 days

replicate 1

Sample_geo_accession	GSM920913
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920913/suppl/GSM920913.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920914

GPL1261

C2 aOBSC rep2

C2 rep2

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 2 days

replicate 2

Sample_geo_accession	GSM920914
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920914/suppl/GSM920914.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920915

GPL1261

C2 aOBSC rep3

C2 rep3

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 2 days

replicate 3

Sample_geo_accession	GSM920915
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920915/suppl/GSM920915.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920916

GPL1261

C4 aOBSC rep1

C4 rep1

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 4 days

replicate 1

Sample_geo_accession	GSM920916
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920916/suppl/GSM920916.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920917

GPL1261

C4 aOBSC rep2

C4 rep2

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 4 days

replicate 2

Sample_geo_accession	GSM920917
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920917/suppl/GSM920917.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

GSM920918

GPL1261

C4 aOBSC rep3

C4 rep3

tissue: olfatory bulb stem cells fgf2 and egf addition frequency: Every 4 days

replicate 3

Sample_geo_accession	GSM920918
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 23 2012
Sample_last_update_date	Apr 27 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from aOBSC cultures using the Trizol reagent (Invitrogen) and purified with Qiagen RNeasy Mini Kit separation columns (Qiagen Cat.: 74104).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA). cDNA was synthesized from 2.5 µg of total RNA using One-cycle target labeling and control reagents (Affymetrix, Santa Clara, CA) to produce biotin labeled cRNA. The cRNA preparation (10 µg) were fragmented at 94ºC for 35 min into 35-200 bases in length.
Sample_hyb_protocol	10 µg of fragmented cRNA were hybridized to the mouse MOE 430 2.0 array (Affymetrix, Santa Clara, CA), containing 39000 transcript variants from 34000 well -characterized mouse genes. Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDTA in the presence of 0.01% of Tween-20 to a final cRNA concentration of 0.05 µg/ml. Hybridization was performed for 16 h at 45ºC . Each microarray was washed and stained with streptavidin-phycoerythrin in a Fluidics station 450 (Affymetrix)
Sample_scan_protocol	Scanned at 1.56 µm resolution in a GeneChip® Scanner 3000 7G System (Affymetrix) Data analyses were performed using GeneChip Operating Software (GCOS).
Sample_data_processing	CEL files were processed using the BioConductor software package (www.bioconductor.org). Normalization was done employing GCRMA (Wu et al., Journal of American Statistical Association, 99(468), 909-917, 2004).
Sample_data_processing	Data postprocessing and graphics were performed using in-house developed functions in Matlab®.
Sample_platform_id	GPL1261
Sample_contact_name	Marcos,J,Araúzo-Bravo
Sample_contact_email	mararabra@yahoo.co.uk
Sample_contact_phone	+49 251 70365 326
Sample_contact_laboratory	Computational Biology and Bioinformatics
Sample_contact_department	Cell and Developmental Biology
Sample_contact_institute	Max Planck Institute for Molecular Biomedicine
Sample_contact_address	Rontgenstr. 20
Sample_contact_city	Muenster
Sample_contact_zip/postal_code	48149
Sample_contact_country	Germany
Sample_contact_web_link	http://www.mpi-muenster.mpg.de/ncd/arauzoe.shtml
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM920nnn/GSM920918/suppl/GSM920918.CEL.gz
Sample_series_id	GSE37516
Sample_data_row_count	45101

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