Search results for the GEO ID: GSE37595  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM923059 | GPL570 |
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siRNA-Tbet
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siRNA-Tbet transfected IL-18 stimulated KG1-cells
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cell line: KG1 cells
transfection: siRNA-Tbet
treatment: stimulated with IL-18
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Gene expression data from siRNA-Tbet transfected IL-18 simulated KG1 cells
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| Sample_geo_accession | GSM923059
| | Sample_status | Public on Oct 30 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Oct 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | KG1 cells were transfected by electroporation either with 1.98µg siRNA-Tbet or with 1.98µg siRNAc. After 5h of rest, transfected cells were stimulated with IL-18 (10ng/ml) for 12h.
| | Sample_growth_protocol_ch1 | Cells were cultivated in RPMI 1640 supplemented with 100U/ml penicillin, 100µg/ml streptomycin and 10% heat-inactivated FCS. All incubations were performed at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA..
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Malte,,Bachmann
| | Sample_contact_email | M.Bachmann@med.uni-frankfurt.de
| | Sample_contact_laboratory | AG Mühl
| | Sample_contact_department | General Pharmacology
| | Sample_contact_institute | University Hospital Frankfurt
| | Sample_contact_address | Theodor Stern Kai 7
| | Sample_contact_city | Frankfurt
| | Sample_contact_zip/postal_code | 60590
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM923nnn/GSM923059/suppl/GSM923059.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM923nnn/GSM923059/suppl/GSM923059.CHP.gz
| | Sample_series_id | GSE37595
| | Sample_data_row_count | 54675
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GSM923060 | GPL570 |
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siRNAc
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siRNAc transfected IL-18 stimulated KG1-cells
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cell line: KG1 cells
transfection: siRNAc
treatment: stimulated with IL-18
|
Gene expression data from siRNAc transfected IL-18 simulated KG1 cells
|
| Sample_geo_accession | GSM923060
| | Sample_status | Public on Oct 30 2012
| | Sample_submission_date | Apr 25 2012
| | Sample_last_update_date | Oct 30 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | KG1 cells were transfected by electroporation either with 1.98µg siRNA-Tbet or with 1.98µg siRNAc. After 5h of rest, transfected cells were stimulated with IL-18 (10ng/ml) for 12h.
| | Sample_growth_protocol_ch1 | Cells were cultivated in RPMI 1640 supplemented with 100U/ml penicillin, 100µg/ml streptomycin and 10% heat-inactivated FCS. All incubations were performed at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA..
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Malte,,Bachmann
| | Sample_contact_email | M.Bachmann@med.uni-frankfurt.de
| | Sample_contact_laboratory | AG Mühl
| | Sample_contact_department | General Pharmacology
| | Sample_contact_institute | University Hospital Frankfurt
| | Sample_contact_address | Theodor Stern Kai 7
| | Sample_contact_city | Frankfurt
| | Sample_contact_zip/postal_code | 60590
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM923nnn/GSM923060/suppl/GSM923060.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM923nnn/GSM923060/suppl/GSM923060.CHP.gz
| | Sample_series_id | GSE37595
| | Sample_data_row_count | 54675
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