Result

Search results for the GEO ID: GSE37620

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM923328

GPL1261

UGM_UNT_24h_rep1

mouse urogenital sinus mesenchyme, untreated (UNT)

strain: C57BL/6 gender: male age: day 16 cell type: urogenital sinus mesenchyme (UGM) cells treatment: none treatment duration: 72 hrs

UGM untreated Male day 16 urogenital sinus mesenchyme from C57BL/6 mice. Cells were grown to confluency and treated for 72h in media containing charcoal-stripped serum before RNA was collected.

Sample_geo_accession	GSM923328
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 26 2012
Sample_last_update_date	Jul 29 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Media containing FBS was removed from flasks. Cells were rinsed with PBS before treatments were added. Cells were treated for 72 hours in RPMI 1640, 5% dextran charcoal-stripped serum, and 5ug/ml Plasmocin (Untreated). Testosterone (12nM)- and estrogen (120pM)-treated cells were also cultured for 72 hours prior to collection.
Sample_growth_protocol_ch1	Cells were grown to 80% confluency in RPMI 1640, 5% FBS, and 5ug/ml Plasmocin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed using the Omega Bio-Tek RNA Extraction Kit, according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cDNA was prepared with kits from NuGEN as recommended by the supplier.
Sample_hyb_protocol	Hybridization, washes, and staining with phycoerythrin-streptavidin (including antibody enhancement step) were carried out as recommended by Affymetrix for eukaryotic expression arrays, using a hybridization oven and fluidics station purchased from Affymetrix.
Sample_scan_protocol	Scans were done with an Affymetrix scanner exactly as recommended by Affymetrix.
Sample_data_processing	Background subtraction and normalization were done with the Bioconductor GCRMA method.
Sample_platform_id	GPL1261
Sample_contact_name	William,A,Ricke
Sample_contact_laboratory	Carbone Cancer Center
Sample_contact_department	Urology
Sample_contact_institute	University of Wisconsin
Sample_contact_address	1111 Highland Ave
Sample_contact_city	Madison
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53705
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM923nnn/GSM923328/suppl/GSM923328_Ricke1_Ricke_M430_2_1.CEL.gz
Sample_series_id	GSE37620
Sample_data_row_count	45101

GSM923329

GPL1261

UGM_TE2_24h_rep1

mouse urogenital sinus mesenchyme, T+E2 treated

strain: C57BL/6 gender: male age: day 16 cell type: urogenital sinus mesenchyme (UGM) cells treatment: testosterone + estradiol-17 beta treatment duration: 72 hrs

UGM T+E treated Male day 16 urogenital sinus mesenchyme from C57BL/6 mice. Cells were grown to near confluency and T+E2 treated for 72h before RNA was collected.

Sample_geo_accession	GSM923329
Sample_status	Public on Apr 27 2012
Sample_submission_date	Apr 26 2012
Sample_last_update_date	Jul 29 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Media containing FBS was removed from flasks. Cells were rinsed with PBS before treatments were added. Cells were treated for 72 hours in RPMI 1640, 5% dextran charcoal-stripped serum, and 5ug/ml Plasmocin (Untreated). Testosterone (12nM)- and estrogen (120pM)-treated cells were also cultured for 72 hours prior to collection.
Sample_growth_protocol_ch1	Cells were grown to 80% confluency in RPMI 1640, 5% FBS, and 5ug/ml Plasmocin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Extraction of total RNA was performed using the Omega Bio-Tek RNA Extraction Kit, according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cDNA was prepared with kits from NuGEN as recommended by the supplier.
Sample_hyb_protocol	Hybridization, washes, and staining with phycoerythrin-streptavidin (including antibody enhancement step) were carried out as recommended by Affymetrix for eukaryotic expression arrays, using a hybridization oven and fluidics station purchased from Affymetrix.
Sample_scan_protocol	Scans were done with an Affymetrix scanner exactly as recommended by Affymetrix.
Sample_data_processing	Background subtraction and normalization were done with the Bioconductor GCRMA method.
Sample_platform_id	GPL1261
Sample_contact_name	William,A,Ricke
Sample_contact_laboratory	Carbone Cancer Center
Sample_contact_department	Urology
Sample_contact_institute	University of Wisconsin
Sample_contact_address	1111 Highland Ave
Sample_contact_city	Madison
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53705
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM923nnn/GSM923329/suppl/GSM923329_Ricke2_Ricke_M430_2_1.CEL.gz
Sample_series_id	GSE37620
Sample_data_row_count	45101

Make groups for comparisons

(2 groups will be compared at a time)

Select GSMs and click on "Add groups"

Enter the group name here:

Select expression type

Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes