Search results for the GEO ID: GSE37676 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM925429 | GPL1261 |
|
Mc3T3 control_ 5day_rep1
|
Mc3T3_untreated_5 days
|
cell line: Mc3T3-E1
cell type: osteoblastic cells
|
Mc3T3-E1_T0-1
MOE4302_042809H_RH1_Ctl1
Gene expression data from control Mc3T3-E1 cells
|
Sample_geo_accession | GSM925429
| Sample_status | Public on May 02 2012
| Sample_submission_date | Apr 30 2012
| Sample_last_update_date | May 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | MC3T3-E1 OB cells grown in 6-well plates and either left untreated or treated with 50 micrograms of Ascorbic Acid (AA) for 5 days. Media was replenished every 2nd day.
| Sample_growth_protocol_ch1 | MC3T3-E1 subclone 4 was obtained from American Type Culture Collection (ATCC). The MC3T3-E1 steoblastic cell line was routinely cultured in α-MEM supplemented with heat inactivated 10% FBS and incubated in a humidified atmosphere at 37oC with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse MOE430 2.0 array.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Noushin,,Nabavi
| Sample_contact_email | harrison@utsc.utoronto.ca
| Sample_contact_institute | University of Toronto
| Sample_contact_address | 1265 Military Trail
| Sample_contact_city | Toronto
| Sample_contact_zip/postal_code | M1C 1A4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925429/suppl/GSM925429_Mc3T3-E1_T0-1.CEL.gz
| Sample_series_id | GSE37676
| Sample_data_row_count | 45101
| |
|
GSM925430 | GPL1261 |
|
Mc3T3 control_ 5day_rep2
|
Mc3T3_untreated_5 days
|
cell line: Mc3T3-E1
cell type: osteoblastic cells
|
Mc3T3-E1_T0-2
MOE4302_042809H_RH3_Ctl2
Gene expression data from control Mc3T3-E1 cells
|
Sample_geo_accession | GSM925430
| Sample_status | Public on May 02 2012
| Sample_submission_date | Apr 30 2012
| Sample_last_update_date | May 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | MC3T3-E1 OB cells grown in 6-well plates and either left untreated or treated with 50 micrograms of Ascorbic Acid (AA) for 5 days. Media was replenished every 2nd day.
| Sample_growth_protocol_ch1 | MC3T3-E1 subclone 4 was obtained from American Type Culture Collection (ATCC). The MC3T3-E1 steoblastic cell line was routinely cultured in α-MEM supplemented with heat inactivated 10% FBS and incubated in a humidified atmosphere at 37oC with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse MOE430 2.0 array.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Noushin,,Nabavi
| Sample_contact_email | harrison@utsc.utoronto.ca
| Sample_contact_institute | University of Toronto
| Sample_contact_address | 1265 Military Trail
| Sample_contact_city | Toronto
| Sample_contact_zip/postal_code | M1C 1A4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925430/suppl/GSM925430_Mc3T3-E1_T0-2.CEL.gz
| Sample_series_id | GSE37676
| Sample_data_row_count | 45101
| |
|
GSM925431 | GPL1261 |
|
Mc3T3 control_ 5day_rep3
|
Mc3T3_untreated_5 days
|
cell line: Mc3T3-E1
cell type: osteoblastic cells
|
Mc3T3-E1_T0-3
MOE4302_042809H_RH5_Ctl3
Gene expression data from control Mc3T3-E1 cells
|
Sample_geo_accession | GSM925431
| Sample_status | Public on May 02 2012
| Sample_submission_date | Apr 30 2012
| Sample_last_update_date | May 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | MC3T3-E1 OB cells grown in 6-well plates and either left untreated or treated with 50 micrograms of Ascorbic Acid (AA) for 5 days. Media was replenished every 2nd day.
| Sample_growth_protocol_ch1 | MC3T3-E1 subclone 4 was obtained from American Type Culture Collection (ATCC). The MC3T3-E1 steoblastic cell line was routinely cultured in α-MEM supplemented with heat inactivated 10% FBS and incubated in a humidified atmosphere at 37oC with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse MOE430 2.0 array.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Noushin,,Nabavi
| Sample_contact_email | harrison@utsc.utoronto.ca
| Sample_contact_institute | University of Toronto
| Sample_contact_address | 1265 Military Trail
| Sample_contact_city | Toronto
| Sample_contact_zip/postal_code | M1C 1A4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925431/suppl/GSM925431_Mc3T3-E1_T0-3.CEL.gz
| Sample_series_id | GSE37676
| Sample_data_row_count | 45101
| |
|
GSM925432 | GPL1261 |
|
Mc3T3 AA_ 5day_rep1
|
Mc3T3 cells_treated with AA_5 days
|
cell line: Mc3T3-E1
cell type: osteoblastic cells
treated with: 50µg of Ascorbic Acid (AA) for 5 days
|
Mc3T3-E1_T1-1
MOE4302_042809H_RH2_AA1
Gene expression data from Mc3T3-E1 cells stimulated with AA for 5 days
|
Sample_geo_accession | GSM925432
| Sample_status | Public on May 02 2012
| Sample_submission_date | Apr 30 2012
| Sample_last_update_date | May 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | MC3T3-E1 OB cells grown in 6-well plates and either left untreated or treated with 50 micrograms of Ascorbic Acid (AA) for 5 days. Media was replenished every 2nd day.
| Sample_growth_protocol_ch1 | MC3T3-E1 subclone 4 was obtained from American Type Culture Collection (ATCC). The MC3T3-E1 steoblastic cell line was routinely cultured in α-MEM supplemented with heat inactivated 10% FBS and incubated in a humidified atmosphere at 37oC with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse MOE430 2.0 array.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Noushin,,Nabavi
| Sample_contact_email | harrison@utsc.utoronto.ca
| Sample_contact_institute | University of Toronto
| Sample_contact_address | 1265 Military Trail
| Sample_contact_city | Toronto
| Sample_contact_zip/postal_code | M1C 1A4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925432/suppl/GSM925432_Mc3T3-E1_T1-1.CEL.gz
| Sample_series_id | GSE37676
| Sample_data_row_count | 45101
| |
|
GSM925433 | GPL1261 |
|
Mc3T3 AA_ 5day_rep2
|
Mc3T3 cells_treated with AA_5 days
|
cell line: Mc3T3-E1
cell type: osteoblastic cells
treated with: 50µg of Ascorbic Acid (AA) for 5 days
|
Mc3T3-E1_T1-2
MOE4302_042809H_RH4_AA2
Gene expression data from Mc3T3-E1 cells stimulated with AA for 5 days
|
Sample_geo_accession | GSM925433
| Sample_status | Public on May 02 2012
| Sample_submission_date | Apr 30 2012
| Sample_last_update_date | May 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | MC3T3-E1 OB cells grown in 6-well plates and either left untreated or treated with 50 micrograms of Ascorbic Acid (AA) for 5 days. Media was replenished every 2nd day.
| Sample_growth_protocol_ch1 | MC3T3-E1 subclone 4 was obtained from American Type Culture Collection (ATCC). The MC3T3-E1 steoblastic cell line was routinely cultured in α-MEM supplemented with heat inactivated 10% FBS and incubated in a humidified atmosphere at 37oC with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse MOE430 2.0 array.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Noushin,,Nabavi
| Sample_contact_email | harrison@utsc.utoronto.ca
| Sample_contact_institute | University of Toronto
| Sample_contact_address | 1265 Military Trail
| Sample_contact_city | Toronto
| Sample_contact_zip/postal_code | M1C 1A4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925433/suppl/GSM925433_Mc3T3-E1_T1-2.CEL.gz
| Sample_series_id | GSE37676
| Sample_data_row_count | 45101
| |
|
GSM925434 | GPL1261 |
|
Mc3T3 AA_ 5day_rep3
|
Mc3T3 cells_treated with AA_5 days
|
cell line: Mc3T3-E1
cell type: osteoblastic cells
treated with: 50µg of Ascorbic Acid (AA) for 5 days
|
Mc3T3-E1_T1-3
MOE4302_042809H_RH6_AA3
Gene expression data from Mc3T3-E1 cells stimulated with AA for 5 days
|
Sample_geo_accession | GSM925434
| Sample_status | Public on May 02 2012
| Sample_submission_date | Apr 30 2012
| Sample_last_update_date | May 02 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | MC3T3-E1 OB cells grown in 6-well plates and either left untreated or treated with 50 micrograms of Ascorbic Acid (AA) for 5 days. Media was replenished every 2nd day.
| Sample_growth_protocol_ch1 | MC3T3-E1 subclone 4 was obtained from American Type Culture Collection (ATCC). The MC3T3-E1 steoblastic cell line was routinely cultured in α-MEM supplemented with heat inactivated 10% FBS and incubated in a humidified atmosphere at 37oC with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse MOE430 2.0 array.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Noushin,,Nabavi
| Sample_contact_email | harrison@utsc.utoronto.ca
| Sample_contact_institute | University of Toronto
| Sample_contact_address | 1265 Military Trail
| Sample_contact_city | Toronto
| Sample_contact_zip/postal_code | M1C 1A4
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925434/suppl/GSM925434_Mc3T3-E1_T1-3.CEL.gz
| Sample_series_id | GSE37676
| Sample_data_row_count | 45101
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|