Search results for the GEO ID: GSE37705  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM925774 | GPL1261 |
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MEFs (Wildtype)
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MEFs from wildtype mouse
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tissue: Embryonic Fibroblast
genotype: Wildtype
age: Embryonic Day 13.5
strain: C57BL/6
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Gene expression data from MEFs
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| Sample_geo_accession | GSM925774
| | Sample_status | Public on Jun 25 2012
| | Sample_submission_date | May 02 2012
| | Sample_last_update_date | Jun 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment was performed.
| | Sample_growth_protocol_ch1 | MEFs were prepared from E13.5 mouse embryos and were maintained for 2 weeks before RNA extraction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Trizol reagent according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled aRNA were prepared from 100 ng total RNA using GeneChip 3’IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the aRNA synthesized from total RNA harvested from MEFs were hybridized to Affymetrix Mouse Genome 430 2.0 arrays. Hybridization was carried out in AFFYMETRIX GeneChip Hybridization Oven 640 at 45°C overnight. All arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | AFFYMETRIX GeneChip Scanner 3000 was used to scan the GeneChips.
| | Sample_data_processing | The data were analyzed with Aligent GeneSpring GX.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuen Yan,Joanna,Ip
| | Sample_contact_laboratory | Nakagawa RNA Biology
| | Sample_contact_department | Advanced Science Institute
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 2-1 Hirosawa
| | Sample_contact_city | Wako
| | Sample_contact_state | Saitama
| | Sample_contact_zip/postal_code | 351-0198
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925774/suppl/GSM925774_Malat1_7WT.CEL.gz
| | Sample_series_id | GSE37705
| | Sample_series_id | GSE37707
| | Sample_data_row_count | 45101
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GSM925775 | GPL1261 |
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MEFs (Malat1 Knock-out)
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MEFs from Malat1 knock-out mouse
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tissue: Embryonic Fibroblast
genotype: Malat1 Knock-out
age: Embryonic Day 13.5
strain: C57BL/6
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Gene expression data from MEFs
|
| Sample_geo_accession | GSM925775
| | Sample_status | Public on Jun 25 2012
| | Sample_submission_date | May 02 2012
| | Sample_last_update_date | Jun 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment was performed.
| | Sample_growth_protocol_ch1 | MEFs were prepared from E13.5 mouse embryos and were maintained for 2 weeks before RNA extraction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with Trizol reagent according to the manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled aRNA were prepared from 100 ng total RNA using GeneChip 3’IVT Express Kit (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the aRNA synthesized from total RNA harvested from MEFs were hybridized to Affymetrix Mouse Genome 430 2.0 arrays. Hybridization was carried out in AFFYMETRIX GeneChip Hybridization Oven 640 at 45°C overnight. All arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | AFFYMETRIX GeneChip Scanner 3000 was used to scan the GeneChips.
| | Sample_data_processing | The data were analyzed with Aligent GeneSpring GX.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Yuen Yan,Joanna,Ip
| | Sample_contact_laboratory | Nakagawa RNA Biology
| | Sample_contact_department | Advanced Science Institute
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 2-1 Hirosawa
| | Sample_contact_city | Wako
| | Sample_contact_state | Saitama
| | Sample_contact_zip/postal_code | 351-0198
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM925nnn/GSM925775/suppl/GSM925775_Malat1_9KO.CEL.gz
| | Sample_series_id | GSE37705
| | Sample_series_id | GSE37707
| | Sample_data_row_count | 45101
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