Search results for the GEO ID: GSE37854  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM929067 | GPL1261 |
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01_-
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unstimlated macrophage
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treatment: none
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| Sample_geo_accession | GSM929067
| | Sample_status | Public on May 20 2012
| | Sample_submission_date | May 08 2012
| | Sample_last_update_date | May 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were injected I.p. with 2ml of 4% thioglycolate (Difco), and peritoneal macrophages were harvested from the peritoneal cavity 3 days after by lavage with cold PBS. Cell were then cultured for 2 hours and stimulated with B-DNA (10μg/ml) or CpG-B(3μM) for 4 hours, and then subjected to microarray analysis.
| | Sample_growth_protocol_ch1 | Mice were maintaind under specific pathogen-free condition in the animal facility of the University of Tokyo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extruced by using Rneasy Mini kit (QIAGEN Cat.No.74104) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, aRNA were hybridized for 16hrs at 45˚C on GeneChip Mouse Genome 430 2.0 Array. Arrays were washed and stained in the Affymetrix® GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix® GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Robust Multichip Array(RMA) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | hideo,,negishi
| | Sample_contact_email | hnegishi@m.u-tokyo.ac.jp
| | Sample_contact_phone | 81-3-5841-3378
| | Sample_contact_fax | 81-3-5841-3450
| | Sample_contact_laboratory | immunology
| | Sample_contact_department | immunology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Tokyo,Bunkyo-ku
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.immunol.m.u-tokyo.ac.jp/page%20files/ja/top/top-ja.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929067/suppl/GSM929067.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929067/suppl/GSM929067.CHP.gz
| | Sample_series_id | GSE37854
| | Sample_data_row_count | 45101
| | |
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GSM929068 | GPL1261 |
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02_B-DNA stimulation 4h
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B-DNA stimulated macrophege
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treatment: B-DNA (10ug/ml) for 4 hr
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| Sample_geo_accession | GSM929068
| | Sample_status | Public on May 20 2012
| | Sample_submission_date | May 08 2012
| | Sample_last_update_date | May 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were injected I.p. with 2ml of 4% thioglycolate (Difco), and peritoneal macrophages were harvested from the peritoneal cavity 3 days after by lavage with cold PBS. Cell were then cultured for 2 hours and stimulated with B-DNA (10μg/ml) or CpG-B(3μM) for 4 hours, and then subjected to microarray analysis.
| | Sample_growth_protocol_ch1 | Mice were maintaind under specific pathogen-free condition in the animal facility of the University of Tokyo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extruced by using Rneasy Mini kit (QIAGEN Cat.No.74104) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, aRNA were hybridized for 16hrs at 45˚C on GeneChip Mouse Genome 430 2.0 Array. Arrays were washed and stained in the Affymetrix® GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix® GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Robust Multichip Array(RMA) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | hideo,,negishi
| | Sample_contact_email | hnegishi@m.u-tokyo.ac.jp
| | Sample_contact_phone | 81-3-5841-3378
| | Sample_contact_fax | 81-3-5841-3450
| | Sample_contact_laboratory | immunology
| | Sample_contact_department | immunology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Tokyo,Bunkyo-ku
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.immunol.m.u-tokyo.ac.jp/page%20files/ja/top/top-ja.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929068/suppl/GSM929068.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929068/suppl/GSM929068.CHP.gz
| | Sample_series_id | GSE37854
| | Sample_data_row_count | 45101
| | |
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GSM929069 | GPL1261 |
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03_CpGB stimulation 4h
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CpG-B stimulated macrophege
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treatment: CpG-B(3uM) for 4 hr
|
|
| Sample_geo_accession | GSM929069
| | Sample_status | Public on May 20 2012
| | Sample_submission_date | May 08 2012
| | Sample_last_update_date | May 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were injected I.p. with 2ml of 4% thioglycolate (Difco), and peritoneal macrophages were harvested from the peritoneal cavity 3 days after by lavage with cold PBS. Cell were then cultured for 2 hours and stimulated with B-DNA (10μg/ml) or CpG-B(3μM) for 4 hours, and then subjected to microarray analysis.
| | Sample_growth_protocol_ch1 | Mice were maintaind under specific pathogen-free condition in the animal facility of the University of Tokyo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extruced by using Rneasy Mini kit (QIAGEN Cat.No.74104) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, aRNA were hybridized for 16hrs at 45˚C on GeneChip Mouse Genome 430 2.0 Array. Arrays were washed and stained in the Affymetrix® GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix® GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Robust Multichip Array(RMA) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | hideo,,negishi
| | Sample_contact_email | hnegishi@m.u-tokyo.ac.jp
| | Sample_contact_phone | 81-3-5841-3378
| | Sample_contact_fax | 81-3-5841-3450
| | Sample_contact_laboratory | immunology
| | Sample_contact_department | immunology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Tokyo,Bunkyo-ku
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.immunol.m.u-tokyo.ac.jp/page%20files/ja/top/top-ja.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929069/suppl/GSM929069.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929069/suppl/GSM929069.CHP.gz
| | Sample_series_id | GSE37854
| | Sample_data_row_count | 45101
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