Search results for the GEO ID: GSE37855  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM929070 | GPL1261 |
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EA0907_07
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unstimlated WT macrophage
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strain: C57B6J
genotype/variation: wild type
cell type: peritoneal macrophage
treatment: none
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| Sample_geo_accession | GSM929070
| | Sample_status | Public on May 20 2012
| | Sample_submission_date | May 08 2012
| | Sample_last_update_date | May 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were injected I.p. with 2ml of 4% thioglycolate (Difco), and peritoneal macrophages were harvested from the peritoneal cavity 3 days after by lavage with cold PBS. Cell were then cultured for 2 hours and infected with VSV (1 M.O.I.) for 6 hours, and then subjected to microarray analysis.
| | Sample_growth_protocol_ch1 | Mice were maintaind under specific pathogen-free condition in the animal facility of the University of Tokyo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extruced by using Rneasy Mini kit (QIAGEN Cat.No.74104) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were synthesized by GeneChip 3'IVT Express Kit（Affymetrix) from 250 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degrees on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| | Sample_data_processing | Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | hideo,,negishi
| | Sample_contact_email | hnegishi@m.u-tokyo.ac.jp
| | Sample_contact_phone | 81-3-5841-3378
| | Sample_contact_fax | 81-3-5841-3450
| | Sample_contact_laboratory | immunology
| | Sample_contact_department | immunology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Tokyo,Bunkyo-ku
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.immunol.m.u-tokyo.ac.jp/page%20files/ja/top/top-ja.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929070/suppl/GSM929070.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929070/suppl/GSM929070.CHP.gz
| | Sample_series_id | GSE37855
| | Sample_data_row_count | 45101
| | |
|
GSM929071 | GPL1261 |
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EA0910_02
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VSV infected WT macrophege
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strain: C57B6J
genotype/variation: wild type
cell type: peritoneal macrophage
treatment: VSV (1 M.O.I.) for 6 hr
|
|
| Sample_geo_accession | GSM929071
| | Sample_status | Public on May 20 2012
| | Sample_submission_date | May 08 2012
| | Sample_last_update_date | May 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were injected I.p. with 2ml of 4% thioglycolate (Difco), and peritoneal macrophages were harvested from the peritoneal cavity 3 days after by lavage with cold PBS. Cell were then cultured for 2 hours and infected with VSV (1 M.O.I.) for 6 hours, and then subjected to microarray analysis.
| | Sample_growth_protocol_ch1 | Mice were maintaind under specific pathogen-free condition in the animal facility of the University of Tokyo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extruced by using Rneasy Mini kit (QIAGEN Cat.No.74104) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were synthesized by GeneChip 3'IVT Express Kit（Affymetrix) from 250 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degrees on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| | Sample_data_processing | Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | hideo,,negishi
| | Sample_contact_email | hnegishi@m.u-tokyo.ac.jp
| | Sample_contact_phone | 81-3-5841-3378
| | Sample_contact_fax | 81-3-5841-3450
| | Sample_contact_laboratory | immunology
| | Sample_contact_department | immunology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Tokyo,Bunkyo-ku
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.immunol.m.u-tokyo.ac.jp/page%20files/ja/top/top-ja.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929071/suppl/GSM929071.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929071/suppl/GSM929071.CHP.gz
| | Sample_series_id | GSE37855
| | Sample_data_row_count | 45101
| | |
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GSM929072 | GPL1261 |
|
EA0910_04
|
VSV infected IRF3-/- macrophege
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strain: C57B6J
genotype/variation: IRF3-/-
cell type: peritoneal macrophage
treatment: VSV (1 M.O.I.) for 6 hr
|
|
| Sample_geo_accession | GSM929072
| | Sample_status | Public on May 20 2012
| | Sample_submission_date | May 08 2012
| | Sample_last_update_date | May 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were injected I.p. with 2ml of 4% thioglycolate (Difco), and peritoneal macrophages were harvested from the peritoneal cavity 3 days after by lavage with cold PBS. Cell were then cultured for 2 hours and infected with VSV (1 M.O.I.) for 6 hours, and then subjected to microarray analysis.
| | Sample_growth_protocol_ch1 | Mice were maintaind under specific pathogen-free condition in the animal facility of the University of Tokyo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extruced by using Rneasy Mini kit (QIAGEN Cat.No.74104) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were synthesized by GeneChip 3'IVT Express Kit（Affymetrix) from 250 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degrees on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| | Sample_data_processing | Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | hideo,,negishi
| | Sample_contact_email | hnegishi@m.u-tokyo.ac.jp
| | Sample_contact_phone | 81-3-5841-3378
| | Sample_contact_fax | 81-3-5841-3450
| | Sample_contact_laboratory | immunology
| | Sample_contact_department | immunology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Tokyo,Bunkyo-ku
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.immunol.m.u-tokyo.ac.jp/page%20files/ja/top/top-ja.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929072/suppl/GSM929072.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929072/suppl/GSM929072.CHP.gz
| | Sample_series_id | GSE37855
| | Sample_data_row_count | 45101
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