Search results for the GEO ID: GSE37868  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM929186 | GPL570 |
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CL1-0 cells expressing control vector
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CL1-0_control vector
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cell line: CL1-0
genotype/variation: expressing control vector
tissue origin: Lung adenocarcinoma
cell type: Type II alveolar cell
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CL1-0
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| Sample_geo_accession | GSM929186
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | May 09 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
| | Sample_growth_protocol_ch1 | Cells were cultured in 5% CO2 incubator at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Hsiao
| | Sample_contact_email | mhsiao@gate.sinica.edu.tw
| | Sample_contact_phone | 886-2-27898752
| | Sample_contact_laboratory | Michael Hsiao Lab
| | Sample_contact_department | Genomics Research Center
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929186/suppl/GSM929186_cl1-0_vector.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929186/suppl/GSM929186_cl1-0_vector.CHP.gz
| | Sample_series_id | GSE37868
| | Sample_data_row_count | 54675
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GSM929187 | GPL570 |
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CL1-0 cells expressing FDFT1
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CL1-0_FDFT overexpression
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cell line: CL1-0
genotype/variation: expressing FDFT1 vector
tissue origin: Lung adenocarcinoma
cell type: Type II alveolar cell
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CL1-0/FDFT1
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| Sample_geo_accession | GSM929187
| | Sample_status | Public on Dec 31 2012
| | Sample_submission_date | May 09 2012
| | Sample_last_update_date | Dec 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
| | Sample_growth_protocol_ch1 | Cells were cultured in 5% CO2 incubator at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Hsiao
| | Sample_contact_email | mhsiao@gate.sinica.edu.tw
| | Sample_contact_phone | 886-2-27898752
| | Sample_contact_laboratory | Michael Hsiao Lab
| | Sample_contact_department | Genomics Research Center
| | Sample_contact_institute | Academia Sinica
| | Sample_contact_address | 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 115
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929187/suppl/GSM929187_cl1-0_fdft1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM929nnn/GSM929187/suppl/GSM929187_cl1-0_fdft1.CHP.gz
| | Sample_series_id | GSE37868
| | Sample_data_row_count | 54675
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