Search results for the GEO ID: GSE37964  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM931035 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 1 ug/ml doxycycline for 8hr, biological replicate #1
|
D7p11 cells, treated with 1 ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 1ug/ml doxycycline to express maximum wild-type dnTCF-1E for 8 hr
|
| Sample_geo_accession | GSM931035
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931035/suppl/GSM931035_0705A-05_D7p11+Dox_A.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931036 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 1 ug/ml doxycycline for 8hr, biological replicate #2
|
D7p11 cells, treated with 1 ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 1ug/ml doxycycline to express maximum wild-type dnTCF-1E for 8 hr
|
| Sample_geo_accession | GSM931036
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931036/suppl/GSM931036_0705A-05_D7p11+Dox_B.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931037 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 1 ug/ml doxycycline for 8hr, biological replicate #3
|
D7p11 cells, treated with 1 ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 1ug/ml doxycycline to express maximum wild-type dnTCF-1E for 8 hr
|
| Sample_geo_accession | GSM931037
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931037/suppl/GSM931037_0705A-05_D7p11+Dox_C.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931038 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 6x10E-4ug/ml doxycycline for 8hr, biological replicate #1
|
D7p11 cells, treated with 6x10E-4ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 6x10E-4ug/ml doxycycline to express wild-type dnTCF-1E for 8hr
|
| Sample_geo_accession | GSM931038
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931038/suppl/GSM931038_0106A-03_8hr_dox_A.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931039 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 6x10E-4ug/ml doxycycline for 8hr, biological replicate #2
|
D7p11 cells, treated with 6x10E-4ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 6x10E-4ug/ml doxycycline to express wild-type dnTCF-1E for 8hr
|
| Sample_geo_accession | GSM931039
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931039/suppl/GSM931039_0106A-03_8hr_dox_B.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931040 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 6x10E-4ug/ml doxycycline for 8hr, biological replicate #3
|
D7p11 cells, treated with 6x10E-4ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 6x10E-4ug/ml doxycycline to express wild-type dnTCF-1E for 8hr
|
| Sample_geo_accession | GSM931040
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931040/suppl/GSM931040_0106A-03_8hr_dox_C.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931041 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 8hr, biological replicate #1
|
D7p11 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water for 8hr, NO expression of the wildtype dnTCF-1E transgene
|
| Sample_geo_accession | GSM931041
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931041/suppl/GSM931041_0106A-03_8hr_no_dox.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931042 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 8hr, biological replicate #2
|
D7p11 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water for 8hr, NO expression of the wildtype dnTCF-1E transgene
|
| Sample_geo_accession | GSM931042
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931042/suppl/GSM931042_0705A-05_D7p11-Dox_A.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931043 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 8hr, biological replicate #3
|
D7p11 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water for 8hr, NO expression of the wildtype dnTCF-1E transgene
|
| Sample_geo_accession | GSM931043
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931043/suppl/GSM931043_0705A-05_D7p11-Dox_B.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931044 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 8hr, biological replicate #4
|
D7p11 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water for 8hr, NO expression of the wildtype dnTCF-1E transgene
|
| Sample_geo_accession | GSM931044
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931044/suppl/GSM931044_0705A-05_D7p11-Dox_C.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931045 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with 1ug/ml doxycycline for 8hr, biological replicate #1
|
MT45 cells, treated with 1ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: mutant dnTCF-1E
|
Expression data from MT45 cells induced with 1ug/ml doxycycline for 8hr to express mutant dnTCF-1E
|
| Sample_geo_accession | GSM931045
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931045/suppl/GSM931045_0705A-05_MT45+Dox_A.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931046 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with 1ug/ml doxycycline for 8hr, biological replicate #2
|
MT45 cells, treated with 1ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: mutant dnTCF-1E
|
Expression data from MT45 cells induced with 1ug/ml doxycycline for 8hr to express mutant dnTCF-1E
|
| Sample_geo_accession | GSM931046
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931046/suppl/GSM931046_0705A-05_MT45+Dox_B.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931047 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with 1ug/ml doxycycline for 8hr, biological replicate #3
|
MT45 cells, treated with 1ug/ml doxycycline for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: mutant dnTCF-1E
|
Expression data from MT45 cells induced with 1ug/ml doxycycline for 8hr to express mutant dnTCF-1E
|
| Sample_geo_accession | GSM931047
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931047/suppl/GSM931047_0705A-05_MT45+Dox_C.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931048 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with water control for 8hr, biological replicate #1
|
MT45 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from MT45 cells induced with water for 8hr, NO expression of the mutant dnTCF-1E transgene
|
| Sample_geo_accession | GSM931048
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931048/suppl/GSM931048_0705A-05_MT45-Dox_A.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931049 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with water control for 8hr, biological replicate #2
|
MT45 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from MT45 cells induced with water for 8hr, NO expression of the mutant dnTCF-1E transgene
|
| Sample_geo_accession | GSM931049
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931049/suppl/GSM931049_0705A-05_MT45-Dox_B.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931050 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with water control for 8hr, biological replicate #3
|
MT45 cells, treated with water control for 8hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from MT45 cells induced with water for 8hr, NO expression of the mutant dnTCF-1E transgene
|
| Sample_geo_accession | GSM931050
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931050/suppl/GSM931050_0705A-05_MT45-Dox_C.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931051 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 1 ug/ml doxycycline for 23hr, biological replicate #1
|
D7p11 cells, treated with 1ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 1ug/ml doxycycline to express maximum wild-type dnTCF-1E for 23 hr
|
| Sample_geo_accession | GSM931051
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931051/suppl/GSM931051_100704A-01_D7P11_wDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931052 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 1 ug/ml doxycycline for 23hr, biological replicate #2
|
D7p11 cells, treated with 1ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 1ug/ml doxycycline to express maximum wild-type dnTCF-1E for 23 hr
|
| Sample_geo_accession | GSM931052
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931052/suppl/GSM931052_121304A-01_D7P11_wDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931053 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 1 ug/ml doxycycline for 23hr, biological replicate #3
|
D7p11 cells, treated with 1ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 1ug/ml doxycycline to express maximum wild-type dnTCF-1E for 23 hr
|
| Sample_geo_accession | GSM931053
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931053/suppl/GSM931053_121304A-01_D7P11_wDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931054 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 6x10E-4ug/ml doxycycline for 23hr, biological replicate #1
|
D7p11 cells, treated with 6x10E-4ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 6x10E-4ug/ml doxycycline to express wild-type dnTCF-1E for 23 hr
|
| Sample_geo_accession | GSM931054
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931054/suppl/GSM931054_0106A-03_23hr_dox_A.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931055 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 6x10E-4ug/ml doxycycline for 23hr, biological replicate #2
|
D7p11 cells, treated with 6x10E-4ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 6x10E-4ug/ml doxycycline to express wild-type dnTCF-1E for 23 hr
|
| Sample_geo_accession | GSM931055
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931055/suppl/GSM931055_0106A-03_23hr_dox_B_ReHyb.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931056 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with 6x10E-4ug/ml doxycycline for 23hr, biological replicate #3
|
D7p11 cells, treated with 6x10E-4ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnTCF-1E
|
Expression data from D7p11 cells induced with 6x10E-4ug/ml doxycycline to express wild-type dnTCF-1E for 23 hr
|
| Sample_geo_accession | GSM931056
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931056/suppl/GSM931056_0106A-03_23hr_dox_C.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931057 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 23hr, biological replicate #1
|
D7p11 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931057
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931057/suppl/GSM931057_0106A-03_23hr_no_dox.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931058 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 23hr, biological replicate #2
|
D7p11 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931058
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931058/suppl/GSM931058_100704A-01_D7P11_noDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931059 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 23hr, biological replicate #3
|
D7p11 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931059
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931059/suppl/GSM931059_121304A-01_D7P11_noDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931060 | GPL570 |
|
wild-type dnTCF-1E inducible cell line, treated with water control for 23hr, biological replicate #4
|
D7p11 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from D7p11 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931060
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931060/suppl/GSM931060_121304A-01_D7P11_noDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931061 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with 1ug/ml doxycycline for 23hr, biological replicate #1
|
MT45 cells, treated with 1 ug/ml doxycycline for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: mutant TCF-1E
|
Expression data from MT45 cells induced with 1 ug/ml doxycycline to express the mutant dnTCF-1E transgene for 23hr
|
| Sample_geo_accession | GSM931061
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931061/suppl/GSM931061_100704A-01_T20_wDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931062 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with 1ug/ml doxycycline for 23hr, biological replicate #2
|
MT45 cells, treated with 1 ug/ml doxycycline for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: mutant TCF-1E
|
Expression data from MT45 cells induced with 1 ug/ml doxycycline to express the mutant dnTCF-1E transgene for 23hr
|
| Sample_geo_accession | GSM931062
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931062/suppl/GSM931062_120704A-01_MT45_wDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931063 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with 1ug/ml doxycycline for 23hr, biological replicate #3
|
MT45 cells, treated with 1 ug/ml doxycycline for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: mutant TCF-1E
|
Expression data from MT45 cells induced with 1 ug/ml doxycycline to express the mutant dnTCF-1E transgene for 23hr
|
| Sample_geo_accession | GSM931063
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931063/suppl/GSM931063_120704A-01_MT45_wDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931064 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with water control for 23hr, biological replicate #1
|
MT45 cells, treated with water control for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from MT45 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931064
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931064/suppl/GSM931064_100704A-01_T20_noDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931065 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with water control for 23hr, biological replicate #2
|
MT45 cells, treated with water control for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from MT45 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931065
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931065/suppl/GSM931065_120704A-01_MT45_noDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931066 | GPL570 |
|
mutant dnTCF-1Emut inducible cell line, treated with water control for 23hr, biological replicate #3
|
MT45 cells, treated with water control for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from MT45 cells induced with water control for 23 hr
|
| Sample_geo_accession | GSM931066
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931066/suppl/GSM931066_120704A-01_MT45_noDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931067 | GPL570 |
|
dnLEF-1 inducible cell line, treated with 1 ug/ml doxycycline for 23 hr, biological replicate #1
|
T20 cells, treated with 1 ug/ml for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnLEF-1
|
Expression data from T20 cells cells induced with 1 ug/ml doxycycline for 23 hr to induce expression of dnLEF-1 transgene
|
| Sample_geo_accession | GSM931067
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931067/suppl/GSM931067_100704A-01_MT45_wDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931068 | GPL570 |
|
dnLEF-1 inducible cell line, treated with 1 ug/ml doxycycline for 23 hr, biological replicate #2
|
T20 cells, treated with 1 ug/ml for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnLEF-1
|
Expression data from T20 cells cells induced with 1 ug/ml doxycycline for 23 hr to induce expression of dnLEF-1 transgene
|
| Sample_geo_accession | GSM931068
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931068/suppl/GSM931068_120704A-01_T20_wDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931069 | GPL570 |
|
dnLEF-1 inducible cell line, treated with 1 ug/ml doxycycline for 23 hr, biological replicate #3
|
T20 cells, treated with 1 ug/ml for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: dnLEF-1
|
Expression data from T20 cells cells induced with 1 ug/ml doxycycline for 23 hr to induce expression of dnLEF-1 transgene
|
| Sample_geo_accession | GSM931069
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931069/suppl/GSM931069_121304A-01_T20_wDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931070 | GPL570 |
|
dnLEF-1 inducible cell line, treated with water control for 23 hr, biological replicate #1
|
T20 cells, treated with water control for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from T20 cells cells induced with water control for 23hr
|
| Sample_geo_accession | GSM931070
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931070/suppl/GSM931070_100704A-01_MT45_noDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931071 | GPL570 |
|
dnLEF-1 inducible cell line, treated with water control for 23 hr, biological replicate #2
|
T20 cells, treated with water control for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from T20 cells cells induced with water control for 23hr
|
| Sample_geo_accession | GSM931071
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931071/suppl/GSM931071_120704A-01_T20_noDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931072 | GPL570 |
|
dnLEF-1 inducible cell line, treated with water control for 23 hr, biological replicate #3
|
T20 cells, treated with water control for 23 hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from T20 cells cells induced with water control for 23hr
|
| Sample_geo_accession | GSM931072
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931072/suppl/GSM931072_121304A-01_T20_noDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931073 | GPL570 |
|
mock cell, treated with 1ug/ml doxycycline for 23h, biological rep1
|
DLD TR7 cells, treated with 1ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from DLD TR7 cells treated with 1ug/ml doxycycline for 23hr-control, but this T-Rex cell line does not express any transgene
|
| Sample_geo_accession | GSM931073
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931073/suppl/GSM931073_100704A-01_OLDTR7_wDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931074 | GPL570 |
|
mock cell, treated with 1ug/ml doxycycline for 23h, biological rep2
|
DLD TR7 cells, treated with 1ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from DLD TR7 cells treated with 1ug/ml doxycycline for 23hr-control, but this T-Rex cell line does not express any transgene
|
| Sample_geo_accession | GSM931074
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931074/suppl/GSM931074_120704A-01_OLD_TR7_wDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931075 | GPL570 |
|
mock cell, treated with 1ug/ml doxycycline for 23h, biological rep3
|
DLD TR7 cells, treated with 1ug/ml doxycycline for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from DLD TR7 cells treated with 1ug/ml doxycycline for 23hr-control, but this T-Rex cell line does not express any transgene
|
| Sample_geo_accession | GSM931075
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931075/suppl/GSM931075_121304A-01_DLD_TR7_wDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931076 | GPL570 |
|
mock cell, treated with water control for 23h, biological rep1
|
DLD TR7 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from DLD TR7 cells induced with water for 23hr. Control T-Rex cell line does not have any transgene
|
| Sample_geo_accession | GSM931076
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931076/suppl/GSM931076_100704A-01_OLDTR7_noDOX.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931077 | GPL570 |
|
mock cell, treated with water control for 23h, biological rep2
|
DLD TR7 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from DLD TR7 cells induced with water for 23hr. Control T-Rex cell line does not have any transgene
|
| Sample_geo_accession | GSM931077
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931077/suppl/GSM931077_120704A-01_OLD_TR7_noDOX_2.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
GSM931078 | GPL570 |
|
mock cell, treated with water control for 23h, biological rep3
|
DLD TR7 cells, treated with water control for 23hr
|
cell type: colon cancer cell, T-REx-regulated clonal stable cell
mutated tumor suppressor: APC (Adenomatous polyposis coli) and p53
constitutive signaling pathway: Wnt signaling pathway
tetracycline/doxycycline-induced protein expression: No
|
Expression data from DLD TR7 cells induced with water for 23hr. Control T-Rex cell line does not have any transgene
|
| Sample_geo_accession | GSM931078
| | Sample_status | Public on Jun 01 2012
| | Sample_submission_date | May 14 2012
| | Sample_last_update_date | Jun 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Three plates per condition were pooled for RNA isolation, and three independent RNA isolations were performed, bringing the total number of independent cultures to 9 plates per condition. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human HG-U133 Plus 2.0 arrays. GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using a GeneChip Scanner 3000.
| | Sample_data_processing | Affymetrix GeneChip measurements were obtained using the robust multi-array average (RMA) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Marian,L.,Waterman
| | Sample_contact_email | marian.waterman@uci.edu
| | Sample_contact_phone | 9498242885
| | Sample_contact_fax | 9498248598
| | Sample_contact_department | Microbiology and Molecular Genetics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 19182 Jamboree Blvd.
| | Sample_contact_city | Irvine
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92697-4025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931078/suppl/GSM931078_121304A-01_DLD_TR7_noDOX_3.CEL.gz
| | Sample_series_id | GSE37964
| | Sample_data_row_count | 54675
| | |
|
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