Search results for the GEO ID: GSE38001 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM931699 | GPL1261 |
|
STHdhQ7/Q7 Heat Shock rep1
|
STHdhQ7/Q7 striatal cells, Heat Shock
|
cell type: striatal cells derived from wild type HdhQ7/Q7 embryonic mice
treatment: cells were heat-shocked at 42°C for six hours
genotype: STHdhQ7/Q7
|
|
Sample_geo_accession | GSM931699
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931699/suppl/GSM931699_STHdhQ7.Q7.HS.1.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931700 | GPL1261 |
|
STHdhQ7/Q7 Heat Shock rep2
|
STHdhQ7/Q7 striatal cells, Heat Shock
|
cell type: striatal cells derived from wild type HdhQ7/Q7 embryonic mice
treatment: cells were heat-shocked at 42°C for six hours
genotype: STHdhQ7/Q7
|
|
Sample_geo_accession | GSM931700
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931700/suppl/GSM931700_STHdhQ7.Q7.HS.2.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931701 | GPL1261 |
|
STHdhQ7/Q7 Heat Shock rep3
|
STHdhQ7/Q7 striatal cells, Heat Shock
|
cell type: striatal cells derived from wild type HdhQ7/Q7 embryonic mice
treatment: cells were heat-shocked at 42°C for six hours
genotype: STHdhQ7/Q7
|
|
Sample_geo_accession | GSM931701
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931701/suppl/GSM931701_STHdhQ7.Q7.HS.3.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931702 | GPL1261 |
|
STHdhQ111/Q111 Heat Shock rep1
|
STHdhQ111/Q111 striatal cells, Heat Shock
|
cell type: striatal cells derived from HdhQ111/Q111 knock-in embryonic mice
treatment: cells were heat-shocked at 42°C for six hours
genotype: STHdhQ111/Q111
|
|
Sample_geo_accession | GSM931702
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931702/suppl/GSM931702_STHdhQ111.Q111.HS.1.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931703 | GPL1261 |
|
STHdhQ111/Q111 Heat Shock rep2
|
STHdhQ111/Q111 striatal cells, Heat Shock
|
cell type: striatal cells derived from HdhQ111/Q111 knock-in embryonic mice
treatment: cells were heat-shocked at 42°C for six hours
genotype: STHdhQ111/Q111
|
|
Sample_geo_accession | GSM931703
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931703/suppl/GSM931703_STHdhQ111.Q111.HS.2.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931704 | GPL1261 |
|
STHdhQ111/Q111 Heat Shock rep3
|
STHdhQ111/Q111 striatal cells, Heat Shock
|
cell type: striatal cells derived from HdhQ111/Q111 knock-in embryonic mice
treatment: cells were heat-shocked at 42°C for six hours
genotype: STHdhQ111/Q111
|
|
Sample_geo_accession | GSM931704
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931704/suppl/GSM931704_STHdhQ111.Q111.HS.3.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931705 | GPL1261 |
|
STHdhQ7/Q7 no treatment rep1
|
STHdhQ7/Q7 striatal cells, no treatment
|
cell type: striatal cells derived from wild type HdhQ7/Q7 embryonic mice
treatment: no treatment
genotype: STHdhQ7/Q7
|
|
Sample_geo_accession | GSM931705
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931705/suppl/GSM931705_STHdhQ7.Q7.1.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931706 | GPL1261 |
|
STHdhQ7/Q7 no treatment rep2
|
STHdhQ7/Q7 striatal cells, no treatment
|
cell type: striatal cells derived from wild type HdhQ7/Q7 embryonic mice
treatment: no treatment
genotype: STHdhQ7/Q7
|
|
Sample_geo_accession | GSM931706
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931706/suppl/GSM931706_STHdhQ7.Q7.2.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931707 | GPL1261 |
|
STHdhQ7/Q7 no treatment rep3
|
STHdhQ7/Q7 striatal cells, no treatment
|
cell type: striatal cells derived from wild type HdhQ7/Q7 embryonic mice
treatment: no treatment
genotype: STHdhQ7/Q7
|
|
Sample_geo_accession | GSM931707
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931707/suppl/GSM931707_STHdhQ7.Q7.3.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931708 | GPL1261 |
|
STHdhQ111/Q111 no treatment rep1
|
STHdhQ111/Q111 striatal cells, no treatment
|
cell type: striatal cells derived from HdhQ111/Q111 knock-in embryonic mice
treatment: no treatment
genotype: STHdhQ111/Q111
|
|
Sample_geo_accession | GSM931708
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931708/suppl/GSM931708_STHdhQ111.Q111.1.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931709 | GPL1261 |
|
STHdhQ111/Q111 no treatment rep2
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STHdhQ111/Q111 striatal cells, no treatment
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cell type: striatal cells derived from HdhQ111/Q111 knock-in embryonic mice
treatment: no treatment
genotype: STHdhQ111/Q111
|
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Sample_geo_accession | GSM931709
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931709/suppl/GSM931709_STHdhQ111.Q111.2.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
|
GSM931710 | GPL1261 |
|
STHdhQ111/Q111 no treatment rep3
|
STHdhQ111/Q111 striatal cells, no treatment
|
cell type: striatal cells derived from HdhQ111/Q111 knock-in embryonic mice
treatment: no treatment
genotype: STHdhQ111/Q111
|
|
Sample_geo_accession | GSM931710
| Sample_status | Public on Jun 19 2012
| Sample_submission_date | May 16 2012
| Sample_last_update_date | Jun 20 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells were heat-shocked at 42°C for six hours
| Sample_growth_protocol_ch1 | STHdhQ7/Q7 and STHdhQ111/Q111 striatal cells derived from wild type HdhQ7/Q7 and HdhQ111/Q111 knock-in embryonic mice were used in this study. Cells were grown at 5% CO2 at 33°C in Dulbecco’s modified Eagle’s medium (DMEM, from CellGro, Manassas, VA) with 10% fetal bovine serum (HyClone, Logan, UT), Pen/Strep/Glutamine and 400 μg/ml G418 (both from CellGro). The cells were only used up to passage 15.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated and purified using Rneasy columns (Qiagen Rneasy plus Mini kit, catalogue no. 74134)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | As recommended by manufacturer
| Sample_hyb_protocol | hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (MOE430v2) following standard techniques.
| Sample_scan_protocol | Scanned with GeneChIP scanner 3000 7G using the standard protocol
| Sample_data_processing | Expression data were analyzed using R and Bioconductor (http://www.bioconductor.org/). We normalized gene expression data using Robust Multichip Average (RMA) algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Laura,,Riva
| Sample_contact_email | lriva@mit.edu
| Sample_contact_laboratory | Fraenkel Lab
| Sample_contact_department | Biological Engineering
| Sample_contact_institute | MIT
| Sample_contact_address | 77 Massachusetts Avenue
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM931nnn/GSM931710/suppl/GSM931710_STHdhQ111.Q111.3.CEL.gz
| Sample_series_id | GSE38001
| Sample_series_id | GSE38002
| Sample_data_row_count | 45101
| |
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