Search results for the GEO ID: GSE38044  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM932887 | GPL1261 |
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Rag Replicate 1
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RAG-2-deficient v-abl-transformed pre-B cells
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genotype: RAG-2-deficient
cell type: G1-phase pre-B cells
treatment: STI571
sample type: control
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7433_420_Rag_rep_1
Mouse ID: R2K Bcl.1
Gene expression from RAG-2-deficient v-abl-transformed pre-B cells.
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| Sample_geo_accession | GSM932887
| | Sample_status | Public on Mar 13 2013
| | Sample_submission_date | May 17 2012
| | Sample_last_update_date | Mar 13 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media, incubated for 48 hr and then harvested.
| | Sample_growth_protocol_ch1 | Cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM), high glucose (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total RNA was amplified as directed in the Affymetrix One-Cycle cDNA Synthesis protocol. Fifteen micrograms of amplified biotin-cRNAs were fragmented.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed using the EukGE-WS2v5 protocol of the Affymetrix Fluidics Station FS450 for antibody amplification.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Operating Software (GCOS; Version 1.4.0.036).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932887/suppl/GSM932887_7433_420_Rag_rep_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932887/suppl/GSM932887_7433_420_Rag_rep_1.CHP.gz
| | Sample_series_id | GSE38044
| | Sample_data_row_count | 45101
| | |
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GSM932888 | GPL1261 |
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Rag Replicate 2
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RAG-2-deficient v-abl-transformed pre-B cells
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genotype: RAG-2-deficient
cell type: G1-phase pre-B cells
treatment: STI571
sample type: control
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7434_420_Rag_rep_2
Mouse ID: R2K Bcl.2
Gene expression from RAG-2-deficient v-abl-transformed pre-B cells.
|
| Sample_geo_accession | GSM932888
| | Sample_status | Public on Mar 13 2013
| | Sample_submission_date | May 17 2012
| | Sample_last_update_date | Mar 13 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media, incubated for 48 hr and then harvested.
| | Sample_growth_protocol_ch1 | Cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM), high glucose (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total RNA was amplified as directed in the Affymetrix One-Cycle cDNA Synthesis protocol. Fifteen micrograms of amplified biotin-cRNAs were fragmented.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed using the EukGE-WS2v5 protocol of the Affymetrix Fluidics Station FS450 for antibody amplification.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Operating Software (GCOS; Version 1.4.0.036).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932888/suppl/GSM932888_7434_420_Rag_rep_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932888/suppl/GSM932888_7434_420_Rag_rep_2.CHP.gz
| | Sample_series_id | GSE38044
| | Sample_data_row_count | 45101
| | |
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GSM932889 | GPL1261 |
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Rag Replicate 3
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RAG-2-deficient v-abl-transformed pre-B cells
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genotype: RAG-2-deficient
cell type: G1-phase pre-B cells
treatment: STI571
sample type: control
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7435_Rag_rep3
Mouse ID: R2K Bcl.3
Gene expression from RAG-2-deficient v-abl-transformed pre-B cells.
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| Sample_geo_accession | GSM932889
| | Sample_status | Public on Mar 13 2013
| | Sample_submission_date | May 17 2012
| | Sample_last_update_date | Mar 13 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media, incubated for 48 hr and then harvested.
| | Sample_growth_protocol_ch1 | Cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM), high glucose (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total RNA was amplified as directed in the Affymetrix One-Cycle cDNA Synthesis protocol. Fifteen micrograms of amplified biotin-cRNAs were fragmented.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed using the EukGE-WS2v5 protocol of the Affymetrix Fluidics Station FS450 for antibody amplification.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Operating Software (GCOS; Version 1.4.0.036).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932889/suppl/GSM932889_7435_Rag_rep3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932889/suppl/GSM932889_7435_Rag_rep3.CHP.gz
| | Sample_series_id | GSE38044
| | Sample_data_row_count | 45101
| | |
|
GSM932890 | GPL1261 |
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Art Replicate 1
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Artemis-deficient v-abl-transformed pre-B cells
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genotype: Artemis-deficient
cell type: G1-phase pre-B cells
treatment: STI571
|
7436_Art 1
Mouse ID: Art 2.1
Gene expression from Artemis-deficient v-abl-transformed pre-B cells.
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| Sample_geo_accession | GSM932890
| | Sample_status | Public on Mar 13 2013
| | Sample_submission_date | May 17 2012
| | Sample_last_update_date | Mar 13 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media, incubated for 48 hr and then harvested.
| | Sample_growth_protocol_ch1 | Cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM), high glucose (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total RNA was amplified as directed in the Affymetrix One-Cycle cDNA Synthesis protocol. Fifteen micrograms of amplified biotin-cRNAs were fragmented.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed using the EukGE-WS2v5 protocol of the Affymetrix Fluidics Station FS450 for antibody amplification.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Operating Software (GCOS; Version 1.4.0.036).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932890/suppl/GSM932890_7436_Art_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932890/suppl/GSM932890_7436_Art_1.CHP.gz
| | Sample_series_id | GSE38044
| | Sample_data_row_count | 45101
| | |
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GSM932891 | GPL1261 |
|
Art Replicate 2
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Artemis-deficient v-abl-transformed pre-B cells
|
genotype: Artemis-deficient
cell type: G1-phase pre-B cells
treatment: STI571
|
7437_Art 2
Mouse ID: Art 5.1
Gene expression from Artemis-deficient v-abl-transformed pre-B cells.
|
| Sample_geo_accession | GSM932891
| | Sample_status | Public on Mar 13 2013
| | Sample_submission_date | May 17 2012
| | Sample_last_update_date | Mar 13 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media, incubated for 48 hr and then harvested.
| | Sample_growth_protocol_ch1 | Cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM), high glucose (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total RNA was amplified as directed in the Affymetrix One-Cycle cDNA Synthesis protocol. Fifteen micrograms of amplified biotin-cRNAs were fragmented.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed using the EukGE-WS2v5 protocol of the Affymetrix Fluidics Station FS450 for antibody amplification.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Operating Software (GCOS; Version 1.4.0.036).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932891/suppl/GSM932891_7437_Art_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932891/suppl/GSM932891_7437_Art_2.CHP.gz
| | Sample_series_id | GSE38044
| | Sample_data_row_count | 45101
| | |
|
GSM932892 | GPL1261 |
|
Art Replicate 3
|
Artemis-deficient v-abl-transformed pre-B cells
|
genotype: Artemis-deficient
cell type: G1-phase pre-B cells
treatment: STI571
|
7438_Art 3
Mouse ID: Art.1 INV4
Gene expression from Artemis-deficient v-abl-transformed pre-B cells.
|
| Sample_geo_accession | GSM932892
| | Sample_status | Public on Mar 13 2013
| | Sample_submission_date | May 17 2012
| | Sample_last_update_date | Mar 13 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells treated with STI571 were passaged with 3 μM STI-571 (Imatinib Mesylate) added to the media, incubated for 48 hr and then harvested.
| | Sample_growth_protocol_ch1 | Cells were maintained in suspension in Dulbecco's Modified Eagle Medium (DMEM), high glucose (Gibco BRL 11960-077) supplemented with 10% fetal bovine serum, 1X Sodium Pyruvate (Gibco BRL 11360-070), 1X Non-Essential Amino Acids (Gibco BRL 11140-050), 1X L-Glutamine (Gibco BRL 25030-081), and 2 μl of beta-mercaptoethanol.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy technology following the manufacturer’s instructions, including the addition of DNase.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total RNA was amplified as directed in the Affymetrix One-Cycle cDNA Synthesis protocol. Fifteen micrograms of amplified biotin-cRNAs were fragmented.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to each array for 16 hours at 45°C in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed using the EukGE-WS2v5 protocol of the Affymetrix Fluidics Station FS450 for antibody amplification.
| | Sample_scan_protocol | Arrays were scanned in an Affymetrix Scanner 3000.
| | Sample_data_processing | Data was obtained using the GeneChip® Operating Software (GCOS; Version 1.4.0.036).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | NIEHS,,Microarray Core
| | Sample_contact_email | microarray@niehs.nih.gov, liuliw@niehs.nih.gov
| | Sample_contact_laboratory | Microarray Core
| | Sample_contact_department | DIR
| | Sample_contact_institute | NIEHS
| | Sample_contact_address | 111 T.W. Alexander Drive
| | Sample_contact_city | RTP
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932892/suppl/GSM932892_7438_Art_3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM932nnn/GSM932892/suppl/GSM932892_7438_Art_3.CHP.gz
| | Sample_series_id | GSE38044
| | Sample_data_row_count | 45101
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