Result

Search results for the GEO ID: GSE38188

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM936400

GPL1261

fetalcellsinlung_1

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e17.5

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936400
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936400/suppl/GSM936400_GE-004_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

GSM936401

GPL1261

fetalcellsinlung_2

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e17.5

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936401
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936401/suppl/GSM936401_GE-005_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

GSM936402

GPL1261

fetalcellsinlung_3

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e16

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936402
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936402/suppl/GSM936402_GE-006_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

GSM936403

GPL1261

fetalcellsinlung_4

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e14

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936403
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936403/suppl/GSM936403_GE-020_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

GSM936404

GPL1261

fetalcellsinlung_5

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e14

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936404
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936404/suppl/GSM936404_GE-021_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

GSM936405

GPL1261

fetalcellsinlung_6

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e15

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936405
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936405/suppl/GSM936405_GE-022_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

GSM936406

GPL1261

fetalcellsinlung_7

Fetal cells flow sorted from the maternal lung

strain: C57BL/6J gestational age of pups: e17.5

Gene expression data from fetal cells collected from the maternal lung in the late stages of pregnancy

Sample_geo_accession	GSM936406
Sample_status	Public on May 31 2012
Sample_submission_date	May 23 2012
Sample_last_update_date	Jun 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Male mice homozygous for the enhanced green fluorescent protein (Egfp) transgene (C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb [stock number 267, Riken BioResource Center, Japan, originally provided by Dr. Masaru Okabe, bred in-house]) were mated to 10-12 week-old wild type C57BL/6J females (stock no. 664, Jackson Labs, Bar Harbor, Maine). With the homozygous male, all pups inherit one copy of the Egfp transgene. Egfp expression was used as a marker for all fetal cells independent of gender.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	GFP+, PI- fetal cells from each set of maternal lungs were sorted directly into the lysis buffer provided with the WT-Ovation One-Direct Amplification system (NuGEN Technologies, Inc., San Carlos, California) at a dilution of 10-40 cells/µl. Cell lysates from each female were pipetted repeatedly and stored at -80°C for 1-8 days following sorting. cDNA was converted and amplified from the RNA in the cell lysates using the WT-Ovation One Direct Amplification system following the manufacturer’s protocol with the exception of using up to 80 cells as input material.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Twenty-five μg cDNA were fragmented and biotinylated using the Encore Biotin Module (NuGEN Techonologies, Inc.)
Sample_hyb_protocol	The arrays were hybridized in an Affymetrix oven at 45°C and 60rpm for 18-40 hours. Arrays were washed using a GeneChip Fluidics Station 450 and stained with streptavidin–phycoerythrin according to the protocol supplied by NuGEN Technologies, Inc. with their Encore Biotin Module.
Sample_scan_protocol	Arrays were scanned with the GeneArray Scanner (Affymetrix).
Sample_data_processing	The arrays were analyzed using the GeneChip Microarray Suite 5.0 (Affymetrix) and normalized in R using quantile-normalization, with ideal mismatch background correction and Tukey biweight summarization.
Sample_platform_id	GPL1261
Sample_contact_name	Stephanie,,Pritchard
Sample_contact_email	stephanie.pritchard@tufts.edu
Sample_contact_phone	617-636-9120
Sample_contact_laboratory	Diana W. Bianchi Lab
Sample_contact_department	Genetics
Sample_contact_institute	Tufts University
Sample_contact_address	800 Washington St, Box 396
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02111
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936406/suppl/GSM936406_GE-024_GFP_pos_Mouse430_2_.CEL.gz
Sample_series_id	GSE38188
Sample_data_row_count	45101

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