Search results for the GEO ID: GSE38215  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM936823 | GPL1261 |
|
control pancreas, biological rep1
|
pancreas, control diet, sham injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Control diet
treatment: sham
|
|
| Sample_geo_accession | GSM936823
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936823/suppl/GSM936823_AFFY1403.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936824 | GPL1261 |
|
control pancreas, biological rep2
|
pancreas, control diet, sham injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Control diet
treatment: sham
|
|
| Sample_geo_accession | GSM936824
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936824/suppl/GSM936824_AFFY1407.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936825 | GPL1261 |
|
control pancreas, biological rep3
|
pancreas, control diet, sham injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Control diet
treatment: sham
|
|
| Sample_geo_accession | GSM936825
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936825/suppl/GSM936825_AFFY1410.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936826 | GPL1261 |
|
STZ pancreas, biological rep1
|
pancreas, control diet, STZ injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Control diet
treatment: streptozotocin
|
|
| Sample_geo_accession | GSM936826
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936826/suppl/GSM936826_AFFY1401.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936827 | GPL1261 |
|
STZ pancreas, biological rep3
|
pancreas, control diet, STZ injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Control diet
treatment: streptozotocin
|
|
| Sample_geo_accession | GSM936827
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936827/suppl/GSM936827_AFFY1405.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936828 | GPL1261 |
|
LP pancreas, biological rep1
|
pancreas, low protein diet, sham injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: sham
|
|
| Sample_geo_accession | GSM936828
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936828/suppl/GSM936828_AFFY1402.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936829 | GPL1261 |
|
LP pancreas, biological rep2
|
pancreas, low protein diet, sham injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: sham
|
|
| Sample_geo_accession | GSM936829
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936829/suppl/GSM936829_AFFY1406.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936830 | GPL1261 |
|
LP pancreas, biological rep3
|
pancreas, low protein diet, sham injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: sham
|
|
| Sample_geo_accession | GSM936830
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936830/suppl/GSM936830_AFFY1409.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936831 | GPL1261 |
|
LPSTZ pancreas, biological rep1
|
pancreas, low protein diet, STZ injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: streptozotocin
|
|
| Sample_geo_accession | GSM936831
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936831/suppl/GSM936831_AFFY1400.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936832 | GPL1261 |
|
LPSTZ pancreas, biological rep2
|
pancreas, low protein diet, STZ injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: streptozotocin
|
|
| Sample_geo_accession | GSM936832
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936832/suppl/GSM936832_AFFY1404.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
| | |
|
GSM936833 | GPL1261 |
|
LPSTZ pancreas, biological rep3
|
pancreas, low protein diet, STZ injected
|
strain: Balb/C
Sex: female
tissue: pancreas
age: 30 days old
maternal diet: Isocaloric low protein diet
treatment: streptozotocin
|
|
| Sample_geo_accession | GSM936833
| | Sample_status | Public on Jun 01 2013
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections were given daily from days 1 to 5 from birth.
| | Sample_growth_protocol_ch1 | Mice were housed in a temperature controlled room with 12 h light:dark cycle. Water and food were given ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted and purified using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 10 ug of total RNA as per the Affymetrix GeneChip Technical Analysis Manual.
| | Sample_hyb_protocol | 10 μg of labeled cRNA was hybridized to Affymetrix Mouse 430 2.0 arrays for 16 hours at 45°C as described in the Affymetrix Technical Analysis Manual. GeneChips were stained with Streptavidin-Phycoerythrin, followed by an antibody solution and a second Streptavidin-Phycoerythrin solution, with all liquid handling performed by a GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned with the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Probe level data from the .CEL files were analyzed using Partek Genomics Suite v6.5. Probes were imported and summarized using RMA and ANOVA was used to determine fold changes and p-values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Aaron,R,Cox
| | Sample_contact_email | arcox2@gmail.com
| | Sample_contact_department | Metabolism and Diabetes
| | Sample_contact_institute | Lawson Health Research Institute
| | Sample_contact_address | 268 Grosvenor St.
| | Sample_contact_city | London
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | N6A 4V2
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936833/suppl/GSM936833_AFFY1408.CEL.gz
| | Sample_series_id | GSE38215
| | Sample_data_row_count | 45101
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