Search results for the GEO ID: GSE38224  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM936970 | GPL1261 |
|
Ring1A/B cKO mock (OHT-)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing an empty vector (OHT-untreated: Ring1A(-/-))
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: an empty vector
treatment: OHT-untreated: Ring1A(-/-)
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing an empty vector (OHT-untreated: Ring1A(-/-))
|
| Sample_geo_accession | GSM936970
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936970/suppl/GSM936970_RSM08612.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936971 | GPL1261 |
|
Ring1A/B cKO mock (OHT+)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing an empty vector (2 days after the start of OHT treatment: Ring1A/B-dKO)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: an empty vector
treatment: OHT treatment: Ring1A/B-dKO
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing an empty vector (2 days after the start of OHT treatment: Ring1A/B-dKO)
|
| Sample_geo_accession | GSM936971
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936971/suppl/GSM936971_RSM08613.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936972 | GPL1261 |
|
Ring1A/B cKO expressing WT Ring1B #1 (OHT-)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #1 (OHT-untreated)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: WT Ring1B construct #1
treatment: OHT-untreated
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #1 (OHT-untreated)
|
| Sample_geo_accession | GSM936972
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936972/suppl/GSM936972_RSM08614.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936973 | GPL1261 |
|
Ring1A/B cKO expressing WT Ring1B #1 (OHT+)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #1 (2 days after the start of OHT treatment)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: WT Ring1B construct #1
treatment: OHT treatment
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #1 (2 days after the start of OHT treatment)
|
| Sample_geo_accession | GSM936973
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936973/suppl/GSM936973_RSM08615.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936974 | GPL1261 |
|
Ring1A/B cKO expressing WT Ring1B #3 (OHT-)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #3 (OHT-untreated)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: WT Ring1B construct #3
treatment: OHT-untreated
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #3 (OHT-untreated)
|
| Sample_geo_accession | GSM936974
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936974/suppl/GSM936974_RSM08616.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936975 | GPL1261 |
|
Ring1A/B cKO expressing WT Ring1B #3 (OHT+)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #3 (2 days after the start of OHT treatment)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: WT Ring1B construct #3
treatment: OHT treatment
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing WT Ring1B construct #3 (2 days after the start of OHT treatment)
|
| Sample_geo_accession | GSM936975
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936975/suppl/GSM936975_RSM08617.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936976 | GPL1261 |
|
Ring1A/B cKO expressing I53S Ring1B #1 (OHT-)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #1 (OHT-untreated)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: I53S Ring1B construct #1
treatment: OHT-untreated
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #1 (OHT-untreated)
|
| Sample_geo_accession | GSM936976
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936976/suppl/GSM936976_RSM08618.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936977 | GPL1261 |
|
Ring1A/B cKO expressing I53S Ring1B #1 (OHT+)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #1 (2 days after the start of OHT treatment)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: I53S Ring1B construct #1
treatment: OHT treatment
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #1 (2 days after the start of OHT treatment)
|
| Sample_geo_accession | GSM936977
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936977/suppl/GSM936977_RSM08619.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936978 | GPL1261 |
|
Ring1A/B cKO expressing I53S Ring1B #4 (OHT-)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #4 (OHT-untreated)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: I53S Ring1B construct #4
treatment: OHT-untreated
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #4 (OHT-untreated)
|
| Sample_geo_accession | GSM936978
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936978/suppl/GSM936978_RSM08620.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936979 | GPL1261 |
|
Ring1A/B cKO expressing I53S Ring1B #4 (OHT+)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #4 (2 days after the start of OHT treatment)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: I53S Ring1B construct #4
treatment: OHT treatment
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53S Ring1B construct #4 (2 days after the start of OHT treatment)
|
| Sample_geo_accession | GSM936979
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936979/suppl/GSM936979_RSM08621.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936980 | GPL1261 |
|
Ring1A/B cKO expressing I53A Ring1B #1 (OHT-)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53A Ring1B construct #1 (OHT-untreated)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: I53A Ring1B construct #1
treatment: OHT-untreated
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53A Ring1B construct #1 (OHT-untreated)
|
| Sample_geo_accession | GSM936980
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936980/suppl/GSM936980_RSM08622.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
|
GSM936981 | GPL1261 |
|
Ring1A/B cKO expressing I53A Ring1B #1 (OHT+)
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53A Ring1B construct #1 (2 days after the start of OHT treatment)
|
genotype/variation: Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2
expression: I53A Ring1B construct #1
treatment: OHT treatment
cell type: Embryonic stem cells
background mouse strain: 129/B6 hybrid
|
Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells expressing I53A Ring1B construct #1 (2 days after the start of OHT treatment)
|
| Sample_geo_accession | GSM936981
| | Sample_status | Public on Jun 11 2012
| | Sample_submission_date | May 24 2012
| | Sample_last_update_date | Jun 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | To deplete endogenous Ring1B, Ring1A(-/-);Ring1B(fl/fl);Rosa26::CreERT2 ES cells stably expressing either of mock, WT or mutant Ring1B construct were treated with 4-hydoxytamoxifen (OHT) at a concentration of 800nM for 24 hours.
| | Sample_growth_protocol_ch1 | ES cells were cultured in DMEM with 20 % fetal bovine serum, MEM nonessential amino acids (Invitrogen), sodium pyruvate (Invitrogen), L-glutamine (Invitrogen), 2-mercaptoethanol (Sigma), and ESGRO (Chemicon) either on irradiated MEF as feeder layers or directly on gelatin-coated surfaces.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The ds-cDNA derived from total RNA was incubated at 37℃ for 16hr with both IVT Labeling NTP mix and IVT Labeling Enzyme mix to synthesize biotin-labeled cRNA.
| | Sample_hyb_protocol | The hybridization cocktail was denatured at 99°C for 5 minutes, incubated at 45°C for 5 minutes, and then injected into a GeneChip Mouse Genome 430 2.0 cartridge. The arrays were incubated at 45°C for 16 hours in a rotating oven at 60 rpm.
| | Sample_scan_protocol | Fluorescence signals on arrays were scanned using the Affymetrix GeneChip Scaner 3000 and information about each probe on the chip was extracted from the image data by the Affymetrix image analysis software, GeneChip Operating Software (GCOS).
| | Sample_data_processing | The Affymetrix Expression Console was used to normalize data and determine signal intensity (RMA-Sketch).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takaho,A.,Endo
| | Sample_contact_email | takaho.endo@riken.jp
| | Sample_contact_laboratory | Laboratory for Integrative Genomics
| | Sample_contact_department | IMS
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | 1-7-22 Suehiro, Tsurumi
| | Sample_contact_city | Yokohama
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM936nnn/GSM936981/suppl/GSM936981_RSM08623.CEL.gz
| | Sample_series_id | GSE38224
| | Sample_series_id | GSE38650
| | Sample_data_row_count | 45101
| | |
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