Search results for the GEO ID: GSE38408 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM941668 | GPL570 |
|
A549 EV control, biological rep 1
|
A549 cells transfected with EV control
|
cell line: A549
transfection: EV control
|
transfected cell line
gene expression data from A549 EV control
|
Sample_geo_accession | GSM941668
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941668/suppl/GSM941668_Bourboulia_1B.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941669 | GPL570 |
|
A549 EV control, biological rep 2
|
A549 cells transfected with EV control
|
cell line: A549
transfection: EV control
|
transfected cell line
gene expression data from A549 EV control
|
Sample_geo_accession | GSM941669
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941669/suppl/GSM941669_Bourboulia_2B.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941670 | GPL570 |
|
A549 EV control, biological rep 3
|
A549 cells transfected with EV control
|
cell line: A549
transfection: EV control
|
transfected cell line
gene expression data from A549 EV control
|
Sample_geo_accession | GSM941670
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941670/suppl/GSM941670_Bourboulia_3B.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941671 | GPL570 |
|
A549 TIMP-2, biological rep 1
|
A549 cells transfected with human cDNA TIMP-2
|
cell line: A549
transfection: TIMP-2
|
transfected cell line
gene expression data from TIMP-2 overexpressing A549 cells
|
Sample_geo_accession | GSM941671
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941671/suppl/GSM941671_Bourboulia_1D.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941672 | GPL570 |
|
A549 TIMP-2, biological rep 2
|
A549 cells transfected with human cDNA TIMP-2
|
cell line: A549
transfection: TIMP-2
|
transfected cell line
gene expression data from TIMP-2 overexpressing A549 cells
|
Sample_geo_accession | GSM941672
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941672/suppl/GSM941672_Bourboulia_2D.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941673 | GPL570 |
|
A549 TIMP-2, biological rep 3
|
A549 cells transfected with human cDNA TIMP-2
|
cell line: A549
transfection: TIMP-2
|
transfected cell line
gene expression data from TIMP-2 overexpressing A549 cells
|
Sample_geo_accession | GSM941673
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941673/suppl/GSM941673_Bourboulia_3D.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941674 | GPL570 |
|
A549 Ala+TIMP-2, biological rep 1
|
A549 cells transfected with human cDNA Ala+TIMP-2
|
cell line: A549
transfection: Ala+TIMP-2
|
transfected cell line
gene expression data from Ala+TIMP-2 overexpressing a549 cells
|
Sample_geo_accession | GSM941674
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941674/suppl/GSM941674_Bourboulia_1E.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941675 | GPL570 |
|
A549 Ala+TIMP-2, biological rep 2
|
A549 cells transfected with human cDNA Ala+TIMP-2
|
cell line: A549
transfection: Ala+TIMP-2
|
transfected cell line
gene expression data from Ala+TIMP-2 overexpressing a549 cells
|
Sample_geo_accession | GSM941675
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941675/suppl/GSM941675_Bourboulia_2E.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941676 | GPL570 |
|
A549 Ala+TIMP-2, biological rep 3
|
A549 cells transfected with human cDNA Ala+TIMP-2
|
cell line: A549
transfection: Ala+TIMP-2
|
transfected cell line
gene expression data from Ala+TIMP-2 overexpressing a549 cells
|
Sample_geo_accession | GSM941676
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941676/suppl/GSM941676_Bourboulia_3E.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941677 | GPL570 |
|
A549 EV control day 21 tumor
|
Tumor xenograft of A549 cells transfected with EV control injected into NOD-SCID mice.
|
parental cell line: A549
transfection: EV control
tissue: tumor xenograft
host: NOD-SCID mouse
|
tumor day 21 post injection
gene expression data from A549 EV control xenograft tumors (pool of 6)
|
Sample_geo_accession | GSM941677
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941677/suppl/GSM941677_Bourboulia_A549EVd21_tumor.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
|
GSM941678 | GPL570 |
|
A549 TIMP-2 day 21 tumor
|
Tumor xenograft of A549 cells transfected with TIMP-2 injected into NOD-SCID mice.
|
parental cell line: A549
transfection: TIMP-2
tissue: tumor xenograft
host: NOD-SCID mouse
|
tumor day 21 post injection
gene expression data from A549 TIMP-2 control xenograft tumors (pool of 6)
|
Sample_geo_accession | GSM941678
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941678/suppl/GSM941678_Bourboulia_A549timp2d21_tumor.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
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GSM941679 | GPL570 |
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A549 Ala+TIMP-2 day 21 tumor
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Tumor xenograft of A549 cells transfected with Ala+TIMP-2 injected into NOD-SCID mice.
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parental cell line: A549
transfection: Ala+TIMP-2
tissue: tumor xenograft
host: NOD-SCID mouse
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tumor day 21 post injection
gene expression data from A549 Ala+TIMP-2 control xenograft tumors (pool of 6)
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Sample_geo_accession | GSM941679
| Sample_status | Public on Jan 28 2013
| Sample_submission_date | Jun 01 2012
| Sample_last_update_date | Jan 29 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | No treatment
| Sample_growth_protocol_ch1 | cells were cultured for 48hrs to 80% confluency.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | FOR CELL LINES: Total RNA from three independent exponential growth phase cell cultures of A549 EV, TIMP-2 and Ala+TIMP-2 was isolated using the RNAeasy kit (Qiagen). FOR TUMORS: Equal amounts of RNA (500ng) from six tumors per group were pooled into one sample and used to perform microarray analysis in tumor xenografts.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | About 100 ng of total RNA samples are reverse transcribed and labeled by biotin. If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_hyb_protocol | If the labeling reaction is successful and passes labeling QC, samples are hybridized overnight to Affymetrix GeneChip microarrays.
| Sample_scan_protocol | Wash/stain/scan of the microarray is carried out under strictly controlled conditions with the Affymetrix Fluidics Station and Scanner.
| Sample_data_processing | The array data were processed using the R/Bioconductor Statistical Package.
| Sample_platform_id | GPL570
| Sample_contact_name | DIMITRA,,BOURBOULIA
| Sample_contact_email | bourmpouliad@mail.nih.gov
| Sample_contact_phone | 3014354588
| Sample_contact_fax | 3014358036
| Sample_contact_department | RADIATION ONCOLOGY BRANCH
| Sample_contact_institute | NIH
| Sample_contact_address | 8717 GROVEMONT CIRCLE
| Sample_contact_city | BETHESDA
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892-4605
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM941nnn/GSM941679/suppl/GSM941679_Bourboulia_A549alat2d21_tumor.CEL.gz
| Sample_series_id | GSE38408
| Sample_data_row_count | 54675
| |
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