Sample_geo_accession | GSM945687
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Sample_status | Public on Jan 07 2013
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Sample_submission_date | Jun 07 2012
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Sample_last_update_date | Jan 07 2013
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_treatment_protocol_ch1 | The brain tissue was minced into small pieces with scalpel on dry ice. The minced tissue were furtherly ground in the mortar with pestle, putting liquid nitrogen during that.
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Sample_growth_protocol_ch1 | The mice were autopsied as a acknowledged protocol by ethical animal committee in Graduate School of Medicine of Kobe University . The cerebrum was dissected from each mouse and immediately frozen in -80 degree.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Trizol extraction of total RNA and purification was performed with TRIzol Plus RNA purification Kit (Invirogen) basically according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were synthesized by GeneChip 3'IVT Express Kit(Affymetrix) from 250 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
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Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degrees on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
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Sample_data_processing | Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
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Sample_platform_id | GPL1261
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Sample_contact_name | Toshiaki,,Sakisaka
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Sample_contact_email | sakisaka@med.kobe-u.ac.jp
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Sample_contact_phone | +81-78-382-5727
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Sample_contact_fax | +81-78-382-5419
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Sample_contact_laboratory | Membrane Dynamics
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Sample_contact_department | Physiology and Cell Biology
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Sample_contact_institute | Kobe University Graduate School of Medicine
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Sample_contact_address | Kusunoki-cho 7-5-1, Chuou-ku
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Sample_contact_city | Kobe
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Sample_contact_state | Hyogo
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Sample_contact_zip/postal_code | 650-0017
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Sample_contact_country | Japan
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM945nnn/GSM945687/suppl/GSM945687_EA0724_01.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM945nnn/GSM945687/suppl/GSM945687_EA0724_01.CHP.gz
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Sample_series_id | GSE38573
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Sample_data_row_count | 45101
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