Search results for the GEO ID: GSE38718 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM948623 | GPL570 |
|
Muscle_Young Women_Rep1
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948623
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948623/suppl/GSM948623_PGEF-Untrained_1314.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948624 | GPL570 |
|
Muscle_Young Women_Rep2
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948624
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948624/suppl/GSM948624_PGEF-Untrained_1315.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948625 | GPL570 |
|
Muscle_Young Women_Rep3
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948625
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948625/suppl/GSM948625_PGEF-Untrained_1322.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948626 | GPL570 |
|
Muscle_Young Women_Rep4
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948626
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948626/suppl/GSM948626_PGEF-Untrained_1336.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948627 | GPL570 |
|
Muscle_Young Women_Rep5
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948627
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948627/suppl/GSM948627_PGEF-Untrained_1339.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948628 | GPL570 |
|
Muscle_Young Women_Rep6
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948628
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948628/suppl/GSM948628_PGEF-Untrained_1328.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948629 | GPL570 |
|
Muscle_Young Women_Rep7
|
Young women resting biceps
|
gender: Female
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948629
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948629/suppl/GSM948629_PGEF-Untrained_1334.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948630 | GPL570 |
|
Muscle_Young Men_Rep1
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948630
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948630/suppl/GSM948630_PGEF-Untrained_1313.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948631 | GPL570 |
|
Muscle_Young Men_Rep2
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948631
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948631/suppl/GSM948631_PGEF-Untrained_1321.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948632 | GPL570 |
|
Muscle_Young Men_Rep3
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948632
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948632/suppl/GSM948632_PGEF-Untrained_1338.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948633 | GPL570 |
|
Muscle_Young Men_Rep4
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948633
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948633/suppl/GSM948633_PGEF-Untrained_1332.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948634 | GPL570 |
|
Muscle_Young Men_Rep5
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948634
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948634/suppl/GSM948634_PGEF-Untrained_1326.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948635 | GPL570 |
|
Muscle_Young Men_Rep6
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948635
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948635/suppl/GSM948635_PGEF-Untrained_1331.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948636 | GPL570 |
|
Muscle_Young Men_Rep7
|
Young men resting biceps
|
gender: Male
age group: Young
|
Gene expression data from young healthy human subject
|
Sample_geo_accession | GSM948636
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948636/suppl/GSM948636_PGEF-Untrained_1337.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948637 | GPL570 |
|
Muscle_Old Women_Rep1
|
Older women resting biceps
|
gender: Female
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948637
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948637/suppl/GSM948637_PGEF-Untrained_20_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948638 | GPL570 |
|
Muscle_Old Women_Rep2
|
Older women resting biceps
|
gender: Female
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948638
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948638/suppl/GSM948638_PGEF-Untrained_16_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948639 | GPL570 |
|
Muscle_Old Women_Rep3
|
Older women resting biceps
|
gender: Female
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948639
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948639/suppl/GSM948639_PGEF-Untrained_19_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948640 | GPL570 |
|
Muscle_Old Women_Rep4
|
Older women resting biceps
|
gender: Female
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948640
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948640/suppl/GSM948640_PGEF-Untrained_13_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948641 | GPL570 |
|
Muscle_Old Men_Rep1
|
Older men resting biceps
|
gender: Male
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948641
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948641/suppl/GSM948641_PGEF-Untrained_21_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948642 | GPL570 |
|
Muscle_Old Men_Rep2
|
Older men resting biceps
|
gender: Male
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948642
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948642/suppl/GSM948642_PGEF-Untrained_17_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948643 | GPL570 |
|
Muscle_Old Men_Rep3
|
Older men resting biceps
|
gender: Male
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948643
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948643/suppl/GSM948643_PGEF-Untrained_22_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
GSM948644 | GPL570 |
|
Muscle_Old Men_Rep4
|
Older men resting biceps
|
gender: Male
age group: Older
|
Gene expression data from older healthy human subject
|
Sample_geo_accession | GSM948644
| Sample_status | Public on Feb 28 2013
| Sample_submission_date | Jun 14 2012
| Sample_last_update_date | Feb 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labeledcDNA was synthesized (EnzoBioarray High Yield RNA transcription Labeling Kit, Affymetrix).
| Sample_hyb_protocol | Biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to manufacturer's instructions. Following hybridization, the probe arrays were washed and stained.
| Sample_scan_protocol | The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent), and the stored images were analyzed using the GeneChip software Microarray Analysis Suite (MAS) 5.0 (Affymetrix, Santa Clara, CA).
| Sample_data_processing | Raw intensity values of all samples were preprocessed and normalized by RMA using Bioconductor. Differentially expressed genes were tested by using an intensity-based Bayesian moderated t-statistic.
| Sample_platform_id | GPL570
| Sample_contact_name | GORDON,,PAUL
| Sample_contact_email | gordonp@med.umich.edu
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 325 E Eisenhower
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48108
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM948nnn/GSM948644/suppl/GSM948644_PGEF-Untrained_18_A60-1UP.CEL.gz
| Sample_series_id | GSE38718
| Sample_data_row_count | 54675
| |
|
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Make groups for comparisons |
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Select GSMs and click on "Add groups" |
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