Search results for the GEO ID: GSE38972  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM953063 | GPL570 |
|
PC3 Control Rep1
|
PC3 cells Untreated
|
cell line: PC3
treatment: none
|
Gene expression data from PC3 cells Untreated
|
| Sample_geo_accession | GSM953063
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953063/suppl/GSM953063_Mollapour_1_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953063/suppl/GSM953063_Mollapour_1_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953064 | GPL570 |
|
PC3 Wee1 Rep1
|
PC3 cells treated with Wee1inhibitorII
|
cell line: PC3
treatment: Wee1 inhibitor II
|
Gene expression data from PC3 cells treated with Wee1inhibitorII
|
| Sample_geo_accession | GSM953064
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953064/suppl/GSM953064_Mollapour_2_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953064/suppl/GSM953064_Mollapour_2_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953065 | GPL570 |
|
PC3 17AAG Rep1
|
PC3 cells treated with 17-AAG
|
cell line: PC3
treatment: 17AAG
|
Gene expression data from PC3 cells treated with 17-AAG
|
| Sample_geo_accession | GSM953065
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953065/suppl/GSM953065_Mollapour_3_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953065/suppl/GSM953065_Mollapour_3_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953066 | GPL570 |
|
PC3 Wee117AAG Rep1
|
PC3 cells treated with Wee1inhibitorII and 17AAG
|
cell line: PC3
treatment: Wee1 inhibitor II and 17AAG
|
Gene expression data from PC3 cells treated with Wee1inhibitorII and 17AAG
|
| Sample_geo_accession | GSM953066
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953066/suppl/GSM953066_Mollapour_4_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953066/suppl/GSM953066_Mollapour_4_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953067 | GPL570 |
|
PC3 Control Rep2 (weak signal)
|
PC3 cells Untreated
|
cell line: PC3
treatment: none
|
Gene expression data from PC3 cells Untreated
|
| Sample_geo_accession | GSM953067
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953067/suppl/GSM953067_Mollapour_5_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953067/suppl/GSM953067_Mollapour_5_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953068 | GPL570 |
|
PC3 Wee1 Rep2
|
PC3 cells treated with Wee1inhibitorII
|
cell line: PC3
treatment: Wee1 inhibitor II
|
Gene expression data from PC3 cells treated with Wee1inhibitorII
|
| Sample_geo_accession | GSM953068
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953068/suppl/GSM953068_Mollapour_6_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953068/suppl/GSM953068_Mollapour_6_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953069 | GPL570 |
|
PC3 17AAG Rep2
|
PC3 cells treated with 17-AAG
|
cell line: PC3
treatment: 17AAG
|
Gene expression data from PC3 cells treated with 17-AAG
|
| Sample_geo_accession | GSM953069
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953069/suppl/GSM953069_Mollapour_7_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953069/suppl/GSM953069_Mollapour_7_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953070 | GPL570 |
|
PC3 Wee117AAG Rep2
|
PC3 cells treated with Wee1inhibitorII and 17AAG
|
cell line: PC3
treatment: Wee1 inhibitor II and 17AAG
|
Gene expression data from PC3 cells treated with Wee1inhibitorII and 17AAG
|
| Sample_geo_accession | GSM953070
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953070/suppl/GSM953070_Mollapour_8_rpt_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953070/suppl/GSM953070_Mollapour_8_rpt_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953071 | GPL570 |
|
PC3 Control Rep3
|
PC3 cells Untreated
|
cell line: PC3
treatment: none
|
Gene expression data from PC3 cells Untreated
|
| Sample_geo_accession | GSM953071
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953071/suppl/GSM953071_Mollapour_9_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953071/suppl/GSM953071_Mollapour_9_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953072 | GPL570 |
|
PC3 Wee1 Rep3
|
PC3 cells treated with Wee1inhibitorII
|
cell line: PC3
treatment: Wee1 inhibitor II
|
Gene expression data from PC3 cells treated with Wee1inhibitorII
|
| Sample_geo_accession | GSM953072
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953072/suppl/GSM953072_Mollapour_10_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953072/suppl/GSM953072_Mollapour_10_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953073 | GPL570 |
|
PC3 17AAG Rep3
|
PC3 cells treated with 17-AAG
|
cell line: PC3
treatment: 17AAG
|
Gene expression data from PC3 cells treated with 17-AAG
|
| Sample_geo_accession | GSM953073
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953073/suppl/GSM953073_Mollapour_11_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953073/suppl/GSM953073_Mollapour_11_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
GSM953074 | GPL570 |
|
PC3 Wee117AAG Rep3
|
PC3 cells treated with Wee1inhibitorII and 17AAG
|
cell line: PC3
treatment: Wee1 inhibitor II and 17AAG
|
Gene expression data from PC3 cells treated with Wee1inhibitorII and 17AAG
|
| Sample_geo_accession | GSM953074
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Jun 27 2012
| | Sample_last_update_date | Jan 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Wee1 inhibitor II was synthesized and 17-AAG was obtained from the National Cancer Institute (Bethesda, MD). Drug treatments were performed as follows unless otherwise stated. Cells were plated in growth media at 10% confluency 1 day prior to initiation of treatment. On the first day of treatment, Wee1 inhibitor was added to the medium so that the final concentration was 2.5 μM. Twentyfour hours later, 17-AAG was added to the medium so that the final concentration was 40 nM. For controls, an equivalent amount of vehicle was administered. Cells were harvested for analysis 48 hrs after administration of 17-AAG.
| | Sample_growth_protocol_ch1 | The human prostate cancer cell line PC3 (ATCC) was cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen). Cells were propagated at 37°C in an atmosphere containing 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNAeasy kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (100 ng) was reverse transcribed and labeled with biotin using Affymetrix 3’ IVTexpress Labeling kit following manufacturer’s protocol.
| | Sample_hyb_protocol | Samples were hybridized to Affymetrix GeneChip microarrays overnight
| | Sample_scan_protocol | GeneChip were scanned on Affymetrix GeneChip scanner 3000
| | Sample_data_processing | Data were collected using Affymetrix AGCC software.Statistical and clustering analysis was performed with Partek Genomics Suite (6.5) software using RMA normalization algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mehdi,,Mollapour
| | Sample_contact_email | mollapourm@mail.nih.gov
| | Sample_contact_phone | 3014436080
| | Sample_contact_department | UOB
| | Sample_contact_institute | NCI-NIH
| | Sample_contact_address | 9000 Rockville Pike
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953074/suppl/GSM953074_Mollapour_12_HG-U133_Plus_2_.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM953nnn/GSM953074/suppl/GSM953074_Mollapour_12_HG-U133_Plus_2_.mas5.CHP.gz
| | Sample_series_id | GSE38972
| | Sample_data_row_count | 54675
| | |
|
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