Search results for the GEO ID: GSE39039  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM954456 | GPL1261 |
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P1CM
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neonatal cardiomyocytes
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strain: C57BL/6J
Sex: male/female
developmental stage: day 1, neonatal
cell type: cardiomyocytes
isolation: dissociation and Percoll separation
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Gene expression data from isolated primary neonatal cardiomyocytes.
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| Sample_geo_accession | GSM954456
| | Sample_status | Public on Jul 06 2012
| | Sample_submission_date | Jun 29 2012
| | Sample_last_update_date | Aug 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The cells were all dissociated by trypsin and collagenase. To separate the cardiomyocytes from cardiac fibroblasts, dissociated cells were expanded on a culture dish and checked the absence of cardiomyocytes by TMRM (Nat Methods 2009 Hattori).
| | Sample_growth_protocol_ch1 | Cardiomyocytes and cardiac fibroblasts freshly obtained from postnatal day 1 and 8-week-old C57BL/6J mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The probe set intensities were quantified using the GeneChip Operating Software (GCOS). All hybridized chips met standard quality control criteria.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mayumi,,Oda
| | Sample_contact_email | moda@a8.keio.jp
| | Sample_contact_fax | +81-3-5363-3293
| | Sample_contact_laboratory | Institute of Integrated Medical Research
| | Sample_contact_department | School of Medicine
| | Sample_contact_institute | Keio University
| | Sample_contact_address | 35 Shinanomachi, Shinjuku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 1608582
| | Sample_contact_country | Japan
| | Sample_contact_web_link | +81-3-5363-3547
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM954nnn/GSM954456/suppl/GSM954456_B6_P1CM100727.CEL.gz
| | Sample_series_id | GSE39039
| | Sample_data_row_count | 45101
| | |
|
GSM954457 | GPL1261 |
|
P1CF
|
neonatal cardiac fibroblasts
|
strain: C57BL/6J
Sex: male/female
developmental stage: day 1, neonatal
cell type: cardiac fibroblasts
isolation: dissociation and Percoll separation
|
Gene expression data from isolated primary neonatal cardiac fibroblasts.
|
| Sample_geo_accession | GSM954457
| | Sample_status | Public on Jul 06 2012
| | Sample_submission_date | Jun 29 2012
| | Sample_last_update_date | Aug 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The cells were all dissociated by trypsin and collagenase. To separate the cardiomyocytes from cardiac fibroblasts, dissociated cells were expanded on a culture dish and checked the absence of cardiomyocytes by TMRM (Nat Methods 2009 Hattori).
| | Sample_growth_protocol_ch1 | Cardiomyocytes and cardiac fibroblasts freshly obtained from postnatal day 1 and 8-week-old C57BL/6J mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The probe set intensities were quantified using the GeneChip Operating Software (GCOS). All hybridized chips met standard quality control criteria.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mayumi,,Oda
| | Sample_contact_email | moda@a8.keio.jp
| | Sample_contact_fax | +81-3-5363-3293
| | Sample_contact_laboratory | Institute of Integrated Medical Research
| | Sample_contact_department | School of Medicine
| | Sample_contact_institute | Keio University
| | Sample_contact_address | 35 Shinanomachi, Shinjuku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 1608582
| | Sample_contact_country | Japan
| | Sample_contact_web_link | +81-3-5363-3547
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM954nnn/GSM954457/suppl/GSM954457_B6_P1CF100727.CEL.gz
| | Sample_series_id | GSE39039
| | Sample_data_row_count | 45101
| | |
|
GSM954458 | GPL1261 |
|
W8CM
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adult cardiomyocytes
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strain: C57BL/6J
Sex: male
developmental stage: 8 weeks, adult
cell type: cardiomyocytes
isolation: dissociation by Langendorf perfusion
|
Gene expression data from isolated primary adult cardiomyocytes.
|
| Sample_geo_accession | GSM954458
| | Sample_status | Public on Jul 06 2012
| | Sample_submission_date | Jun 29 2012
| | Sample_last_update_date | Aug 14 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The cells were all dissociated by trypsin and collagenase. To separate the cardiomyocytes from cardiac fibroblasts, dissociated cells were expanded on a culture dish and checked the absence of cardiomyocytes by TMRM (Nat Methods 2009 Hattori).
| | Sample_growth_protocol_ch1 | Cardiomyocytes and cardiac fibroblasts freshly obtained from postnatal day 1 and 8-week-old C57BL/6J mice.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The probe set intensities were quantified using the GeneChip Operating Software (GCOS). All hybridized chips met standard quality control criteria.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mayumi,,Oda
| | Sample_contact_email | moda@a8.keio.jp
| | Sample_contact_fax | +81-3-5363-3293
| | Sample_contact_laboratory | Institute of Integrated Medical Research
| | Sample_contact_department | School of Medicine
| | Sample_contact_institute | Keio University
| | Sample_contact_address | 35 Shinanomachi, Shinjuku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 1608582
| | Sample_contact_country | Japan
| | Sample_contact_web_link | +81-3-5363-3547
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM954nnn/GSM954458/suppl/GSM954458_B6_W8CM100727.CEL.gz
| | Sample_series_id | GSE39039
| | Sample_data_row_count | 45101
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