Search results for the GEO ID: GSE39186  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM957688 | GPL570 |
|
1.TET1-A_+Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET1
treatment: induced
|
Gene expression data from TET1-A clone, 48 h after induced expression of the integrated TET1 gene
|
| Sample_geo_accession | GSM957688
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957688/suppl/GSM957688_1.TET1-A_+Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957688/suppl/GSM957688_1.TET1-A_+Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957689 | GPL570 |
|
2.TET1-F_+Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET1
treatment: induced
|
Gene expression data from TET1-F clone, 48 h after induced expression of the integrated TET1 gene
|
| Sample_geo_accession | GSM957689
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957689/suppl/GSM957689_2.TET1-F_+Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957689/suppl/GSM957689_2.TET1-F_+Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957690 | GPL570 |
|
3.TET1-G_+Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET1
treatment: induced
|
Gene expression data from TET1-G clone, 48 h after induced expression of the integrated TET1 gene
|
| Sample_geo_accession | GSM957690
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957690/suppl/GSM957690_3.TET1-G_+Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957690/suppl/GSM957690_3.TET1-G_+Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957691 | GPL570 |
|
4.TET1-A_-Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET1
treatment: uninduced
|
Gene expression data from TET1-A clone, not induced
|
| Sample_geo_accession | GSM957691
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957691/suppl/GSM957691_4.TET1-A_-Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957691/suppl/GSM957691_4.TET1-A_-Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957692 | GPL570 |
|
5.TET1-F_-Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET1
treatment: uninduced
|
Gene expression data from TET1-F clone, not induced
|
| Sample_geo_accession | GSM957692
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957692/suppl/GSM957692_5.TET1-F_-Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957692/suppl/GSM957692_5.TET1-F_-Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957693 | GPL570 |
|
6.TET1-G_-Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET1
treatment: uninduced
|
Gene expression data from TET1-G clone, not induced
|
| Sample_geo_accession | GSM957693
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957693/suppl/GSM957693_6.TET1-G_-Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957693/suppl/GSM957693_6.TET1-G_-Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957694 | GPL570 |
|
7.TET3-A_+Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET3
treatment: induced
|
Gene expression data from TET3-A clone, 48 h after induced expression of the integrated TET3 gene
|
| Sample_geo_accession | GSM957694
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957694/suppl/GSM957694_7.TET3-A_+Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957694/suppl/GSM957694_7.TET3-A_+Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957695 | GPL570 |
|
8.TET3-B_+Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET3
treatment: induced
|
Gene expression data from TET3-B clone, 48 h after induced expression of the integrated TET3 gene
|
| Sample_geo_accession | GSM957695
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957695/suppl/GSM957695_8.TET3-B_+Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957695/suppl/GSM957695_8.TET3-B_+Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957696 | GPL570 |
|
9.TET3-C_+Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET3
treatment: induced
|
Gene expression data from TET3-C clone, 48 h after induced expression of the integrated TET3 gene
|
| Sample_geo_accession | GSM957696
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957696/suppl/GSM957696_9.TET3-C_+Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957696/suppl/GSM957696_9.TET3-C_+Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957697 | GPL570 |
|
10.TET3-A_-Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET3
treatment: uninduced
|
Gene expression data from TET3-A clone, not induced
|
| Sample_geo_accession | GSM957697
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957697/suppl/GSM957697_10.TET3-A_-Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957697/suppl/GSM957697_10.TET3-A_-Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957698 | GPL570 |
|
11.TET3-B_-Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET3
treatment: uninduced
|
Gene expression data from TET3-B clone, not induced
|
| Sample_geo_accession | GSM957698
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957698/suppl/GSM957698_11.TET3-B_-Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957698/suppl/GSM957698_11.TET3-B_-Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
|
GSM957699 | GPL570 |
|
12.TET3-C_-Dox
|
Flp-In™ T-REx™ 293
|
cell type: Flp-In™ T-REx™ 293
transgene: TET3
treatment: uninduced
|
Gene expression data from TET3-C clone, not induced
|
| Sample_geo_accession | GSM957699
| | Sample_status | Public on Jul 09 2013
| | Sample_submission_date | Jul 09 2012
| | Sample_last_update_date | Jul 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Doxycycline was used to induce the expression of the integrated TET1 or TET3 copy.
| | Sample_growth_protocol_ch1 | Cells were cultured in DMEM supplemented with 10% FCS, 1% Pen/Strep and appropriate concentrations of selective antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the AllPrep DNA/RNA/Protein Mini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix ExpressKit protocol starting from 200 ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133Plus_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Affymetrix Hybridization, Wash and Stain Kit..
| | Sample_scan_protocol | Arrays were scanned in a GeneArray Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling to a target intensity of 1000 as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ludger,,Klein-Hitpass
| | Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| | Sample_contact_phone | +49 201 723 85552
| | Sample_contact_laboratory | BioChip Lab
| | Sample_contact_department | Universitaetsklinikum
| | Sample_contact_institute | Institut fuer Zellbiologie
| | Sample_contact_address | Virchowstr. 173
| | Sample_contact_city | Essen
| | Sample_contact_zip/postal_code | D-45122
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957699/suppl/GSM957699_12.TET3-C_-Dox.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM957nnn/GSM957699/suppl/GSM957699_12.TET3-C_-Dox.CHP.gz
| | Sample_series_id | GSE39186
| | Sample_data_row_count | 54675
| | |
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