Search results for the GEO ID: GSE39382  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM866205 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3, rep 1
|
mouse bone marrow-derived mast cells treated with IL3
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3, rep 1 of 6.
|
| Sample_geo_accession | GSM866205
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Jul 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866205/suppl/GSM866205.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866205/suppl/GSM866205.CHP.gz
| | Sample_series_id | GSE35332
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866206 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3, rep 2
|
mouse bone marrow-derived mast cells treated with IL3
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3, rep 2 of 6.
|
| Sample_geo_accession | GSM866206
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Jul 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866206/suppl/GSM866206.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866206/suppl/GSM866206.CHP.gz
| | Sample_series_id | GSE35332
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866207 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3, rep 3
|
mouse bone marrow-derived mast cells treated with IL3
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3, rep 3 of 6.
|
| Sample_geo_accession | GSM866207
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Jul 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866207/suppl/GSM866207.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866207/suppl/GSM866207.CHP.gz
| | Sample_series_id | GSE35332
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866208 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3, rep 4
|
mouse bone marrow-derived mast cells treated with IL3
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3, rep 4 of 6.
|
| Sample_geo_accession | GSM866208
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Jul 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866208/suppl/GSM866208.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866208/suppl/GSM866208.CHP.gz
| | Sample_series_id | GSE35332
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866209 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3, rep 5
|
mouse bone marrow-derived mast cells treated with IL3
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3, rep 5 of 6.
|
| Sample_geo_accession | GSM866209
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Jul 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866209/suppl/GSM866209.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866209/suppl/GSM866209.CHP.gz
| | Sample_series_id | GSE35332
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866210 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3, rep 6
|
mouse bone marrow-derived mast cells treated with IL3
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3, rep 6 of 6.
|
| Sample_geo_accession | GSM866210
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Jul 16 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866210/suppl/GSM866210.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866210/suppl/GSM866210.CHP.gz
| | Sample_series_id | GSE35332
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866211 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3+IL33, rep 1
|
mouse bone marrow-derived mast cells treated with IL3+IL33
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3 and interleukin 33
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3+IL33, rep 1 of 6.
|
| Sample_geo_accession | GSM866211
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ) and mouse IL-33 (10 ng/ml). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866211/suppl/GSM866211.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866211/suppl/GSM866211.CHP.gz
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866212 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3+IL33, rep 2
|
mouse bone marrow-derived mast cells treated with IL3+IL33
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3 and interleukin 33
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3+IL33, rep 2 of 6.
|
| Sample_geo_accession | GSM866212
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ) and mouse IL-33 (10 ng/ml). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866212/suppl/GSM866212.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866212/suppl/GSM866212.CHP.gz
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866213 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3+IL33, rep 3
|
mouse bone marrow-derived mast cells treated with IL3+IL33
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3 and interleukin 33
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3+IL33, rep 3 of 6.
|
| Sample_geo_accession | GSM866213
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ) and mouse IL-33 (10 ng/ml). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866213/suppl/GSM866213.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866213/suppl/GSM866213.CHP.gz
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866214 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3+IL33, rep 4
|
mouse bone marrow-derived mast cells treated with IL3+IL33
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3 and interleukin 33
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3+IL33, rep 4 of 6.
|
| Sample_geo_accession | GSM866214
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ) and mouse IL-33 (10 ng/ml). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866214/suppl/GSM866214.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866214/suppl/GSM866214.CHP.gz
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866215 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3+IL33, rep 5
|
mouse bone marrow-derived mast cells treated with IL3+IL33
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3 and interleukin 33
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3+IL33, rep 5 of 6.
|
| Sample_geo_accession | GSM866215
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ) and mouse IL-33 (10 ng/ml). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866215/suppl/GSM866215.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866215/suppl/GSM866215.CHP.gz
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
|
GSM866216 | GPL1261 |
|
Mouse bone marrow-derived mast cells treated with IL3+IL33, rep 6
|
mouse bone marrow-derived mast cells treated with IL3+IL33
|
strain: C57BL/6
cell type: bone marrow-derived mast cells
treatment: interleukin 3 and interleukin 33
|
Gene expression data from mouse bone marrow-derived mast cells treated with IL3+IL33, rep 6 of 6.
|
| Sample_geo_accession | GSM866216
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Jan 25 2012
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Essentially, the cells were cultured for 4-6 weeks in media containing mouse recombinant IL-3 (30 ng/ml) (Peprotech, Rocky Hill, NJ) and mouse IL-33 (10 ng/ml). The cells were maintained at 37 ˚C in a humidified incubator gassed with 95% air and 5% CO2. The purity of the cultures, as assessed by toluidine blue staining and FceRIa and KIT expression, was >99%.
| | Sample_growth_protocol_ch1 | Bone marrow-derived mast cells (BMMCs) were developed from bone marrow obtained from the femurs of C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME) as described (Kuehn, H. S., M. A. Beaven, H. T. Ma, M. S. Kim, D. D. Metcalfe, and A. M. Gilfillan. 2008. Synergistic activation of phospholipases Cγ and Cβ: a novel mechanism for PI3K-independent enhancement of FcεRI-induced mast cell mediator release. Cell Signal 20:625-636 (PMID 18207701)).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were extracted using an RNeasy 96 kit (Qiagen, Valencia, CA) with each sample treated with 27 units DNase I during the extraction process.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix GeneChip targets were made by amplification of 50 ng RNA using the WT-Ovation RNA Amplification System (Nugen Inc., San Carlos, CA).
| | Sample_hyb_protocol | Hybridization and fluidics were performed according to standard Affymetrix protocols (http://www.affymetrix.com).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus according to standard Affymetrix protocols.
| | Sample_data_processing | The data were analyzed with GCOS 1.4 and EC 1.1 using Affymetrix default settings except the trimmed mean target intensity of each array was arbitrarily set to 1250.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Dan,,Sturdevant
| | Sample_contact_email | dsturdevant@niaid.nih.gov
| | Sample_contact_phone | 4063639248
| | Sample_contact_institute | NIH
| | Sample_contact_address | -
| | Sample_contact_city | Hamilton
| | Sample_contact_state | MT
| | Sample_contact_zip/postal_code | 59840
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866216/suppl/GSM866216.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM866nnn/GSM866216/suppl/GSM866216.CHP.gz
| | Sample_series_id | GSE39382
| | Sample_data_row_count | 45101
| | |
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