Search results for the GEO ID: GSE39592 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM972664 | GPL1261 |
|
WT CD4 T cells, vehicle-treated, biological rep1
|
WT CD4 T cells treated with veh
|
tissue: spleen
genotype/variation: wild type
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with vehicle
|
Sample_geo_accession | GSM972664
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972664/suppl/GSM972664_WT_med_1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972664/suppl/GSM972664_WT_med_1.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972665 | GPL1261 |
|
WT CD4 T cells, vehicle-treated, biological rep2
|
WT CD4 T cells treated with veh
|
tissue: spleen
genotype/variation: wild type
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with vehicle
|
Sample_geo_accession | GSM972665
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972665/suppl/GSM972665_WT_med_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972665/suppl/GSM972665_WT_med_2.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972666 | GPL1261 |
|
WT CD4 T cells, cAMP-treated, biological rep1
|
WT CD4 T cells treated with db-cAMP
|
tissue: spleen
genotype/variation: wild type
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with db-cAMP
|
Sample_geo_accession | GSM972666
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972666/suppl/GSM972666_WT_cAMP_1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972666/suppl/GSM972666_WT_cAMP_1.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972667 | GPL1261 |
|
WT CD4 T cells, cAMP-treated, biological rep2
|
WT CD4 T cells treated with db-cAMP
|
tissue: spleen
genotype/variation: wild type
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with db-cAMP
|
Sample_geo_accession | GSM972667
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972667/suppl/GSM972667_WT_cAMP_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972667/suppl/GSM972667_WT_cAMP_2.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972668 | GPL1261 |
|
IFN-gR1-KO CD4 T cells, vehicle-treated, biological rep1
|
KO CD4 T cells treated with veh
|
tissue: spleen
genotype/variation: IFN-gR1 KO
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with vehicle
|
Sample_geo_accession | GSM972668
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972668/suppl/GSM972668_KO_med_1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972668/suppl/GSM972668_KO_med_1.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972669 | GPL1261 |
|
IFN-gR1-KO CD4 T cells, vehicle-treated, biological rep2
|
KO CD4 T cells treated with veh
|
tissue: spleen
genotype/variation: IFN-gR1 KO
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with vehicle
|
Sample_geo_accession | GSM972669
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972669/suppl/GSM972669_KO_med_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972669/suppl/GSM972669_KO_med_2.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972670 | GPL1261 |
|
IFN-gR1-KO CD4 T cells, cAMP-treated, biological rep1
|
KO CD4 T cells treated with db-cAMP
|
tissue: spleen
genotype/variation: IFN-gR1 KO
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with db-cAMP
|
Sample_geo_accession | GSM972670
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972670/suppl/GSM972670_KO_cAMP_1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972670/suppl/GSM972670_KO_cAMP_1.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
GSM972671 | GPL1261 |
|
IFN-gR1-KO CD4 T cells, cAMP-treated, biological rep2
|
KO CD4 T cells treated with db-cAMP
|
tissue: spleen
genotype/variation: IFN-gR1 KO
age: 8 weeks
cell type: CD4 T cells
|
Gene expression data from TCR-activated WT CD4 T cells treated with db-cAMP
|
Sample_geo_accession | GSM972671
| Sample_status | Public on Jul 24 2012
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 24 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | db-cAMP or vehicle control (med) was added into cultures for the last 3 h.
| Sample_growth_protocol_ch1 | CD4+ T cells were purified from spleens of WT or IFN-gR1-deficient mice by autoMACS, and activated with anti-CD3 plus anti-CD28 for 15 h.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was purified by Qiagen Rneasy mini kit.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol by GeneChip 3' IVT Express Kit (Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chengcan,,Yao
| Sample_contact_email | yao@mfour.med.kyoyo-u.ac.jp
| Sample_contact_phone | +81-75-753-4395
| Sample_contact_fax | +81-75-753-4693
| Sample_contact_department | Pharmacology
| Sample_contact_institute | Kyoto University
| Sample_contact_address | Yoshida, Sakyo-ku
| Sample_contact_city | Kyoto
| Sample_contact_zip/postal_code | 606-8501
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972671/suppl/GSM972671_KO_cAMP_2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972671/suppl/GSM972671_KO_cAMP_2.CHP.gz
| Sample_series_id | GSE39592
| Sample_data_row_count | 45101
| |
|
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