Search results for the GEO ID: GSE39594 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM972736 | GPL570 |
|
T0_Control.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 0 hours
|
|
Sample_geo_accession | GSM972736
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972736/suppl/GSM972736_25.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972736/suppl/GSM972736_25.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972737 | GPL570 |
|
T24_Control.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972737
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972737/suppl/GSM972737_26.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972737/suppl/GSM972737_26.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972738 | GPL570 |
|
T24_CD3.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972738
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972738/suppl/GSM972738_27.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972738/suppl/GSM972738_27.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972739 | GPL570 |
|
T24_CD3CD28.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3 + anti-CD28
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972739
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972739/suppl/GSM972739_28.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972739/suppl/GSM972739_28.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972740 | GPL570 |
|
T0_Control.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 0 hours
|
|
Sample_geo_accession | GSM972740
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972740/suppl/GSM972740_33.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972740/suppl/GSM972740_33.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972741 | GPL570 |
|
T24_Control.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972741
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972741/suppl/GSM972741_34.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972741/suppl/GSM972741_34.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972742 | GPL570 |
|
T24_CD3.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972742
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972742/suppl/GSM972742_35.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972742/suppl/GSM972742_35.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972743 | GPL570 |
|
T24_CD3CD28.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3 + anti-CD28
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972743
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972743/suppl/GSM972743_36.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972743/suppl/GSM972743_36.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972744 | GPL570 |
|
T0_Control.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 0 hours
|
|
Sample_geo_accession | GSM972744
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972744/suppl/GSM972744_41.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972744/suppl/GSM972744_41.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972745 | GPL570 |
|
T24_Control.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972745
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972745/suppl/GSM972745_42.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972745/suppl/GSM972745_42.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972746 | GPL570 |
|
T24_CD3.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972746
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972746/suppl/GSM972746_43.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972746/suppl/GSM972746_43.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972747 | GPL570 |
|
T24_CD3CD28.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3 + anti-CD28
treatment time: 24 hours
|
|
Sample_geo_accession | GSM972747
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972747/suppl/GSM972747_44.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972747/suppl/GSM972747_44.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972748 | GPL570 |
|
T4_Control.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972748
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972748/suppl/GSM972748_45.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972748/suppl/GSM972748_45.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972749 | GPL570 |
|
T4_CD3.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972749
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972749/suppl/GSM972749_46.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972749/suppl/GSM972749_46.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972750 | GPL570 |
|
T4_CD3CD28.rep1
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3 + anti-CD28
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972750
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972750/suppl/GSM972750_47.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972750/suppl/GSM972750_47.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972751 | GPL570 |
|
T4_Control.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972751
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972751/suppl/GSM972751_49.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972751/suppl/GSM972751_49.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972752 | GPL570 |
|
T4_CD3.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972752
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972752/suppl/GSM972752_50.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972752/suppl/GSM972752_50.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972753 | GPL570 |
|
T4_CD3CD28.rep2
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3 + anti-CD28
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972753
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972753/suppl/GSM972753_51.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972753/suppl/GSM972753_51.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972754 | GPL570 |
|
T4_Control.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: Control
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972754
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972754/suppl/GSM972754_57.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972754/suppl/GSM972754_57.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972755 | GPL570 |
|
T4_CD3.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972755
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972755/suppl/GSM972755_58.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972755/suppl/GSM972755_58.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
| |
|
GSM972756 | GPL570 |
|
T4_CD3CD28.rep3
|
naïve T cells from blood
|
tissue: blood
cell type: CD4+CD45RA+ T cells
treatment: anti-CD3 + anti-CD28
treatment time: 4 hours
|
|
Sample_geo_accession | GSM972756
| Sample_status | Public on Jul 03 2013
| Sample_submission_date | Jul 23 2012
| Sample_last_update_date | Jul 03 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The cells were activated with latex beads conjugates to control antibody or antibodies specific for anti-CD3 or to both anti-CD3 and anti-CD28.
| Sample_growth_protocol_ch1 | Human naïve CD4+ T cells were isolated by FACS sorter and seeded into a 12-well plates at a density of 1x10^6 cells per ml of RMPI-1640 supplemented with 5% human heat-inactivated pooled AB serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_hyb_protocol | Hybridization protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning protocol was performed by the Genome Core of the J. David Gladstone Institutes according to the manufacturer's instructions.
| Sample_data_processing | RMA normalization
| Sample_platform_id | GPL570
| Sample_contact_name | MARC,,MARTINEZ-LLORDELLA
| Sample_contact_email | marc.martinez-llordella@kcl.ac.uk
| Sample_contact_laboratory | BLUESTONE LAB
| Sample_contact_department | DIABETES CENTER
| Sample_contact_institute | UCSF
| Sample_contact_address | 513 PARNASSUS AVE. HSW1102
| Sample_contact_city | SAN FRANCSICO
| Sample_contact_state | California
| Sample_contact_zip/postal_code | 94143
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972756/suppl/GSM972756_59.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM972nnn/GSM972756/suppl/GSM972756_59.a_.rma.chp.gz
| Sample_series_id | GSE39594
| Sample_series_id | GSE39596
| Sample_data_row_count | 54675
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Make groups for comparisons |
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Select GSMs and click on "Add groups" |
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