Search results for the GEO ID: GSE39718  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM978175 | GPL570 |
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MDA-MB-231 control ETOH (vehicle)
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MDA-MB-231 cells, treated for 3 hours with 0.1% Ethanol
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cell line: MDA-MB-231
protocol: ethanol treated cells (vehicle) 3h
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| Sample_geo_accession | GSM978175
| | Sample_status | Public on Jul 31 2012
| | Sample_submission_date | Jul 30 2012
| | Sample_last_update_date | Jul 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with Estradiol or ERα17p for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_data_processing | Summarization Algorithm: RMA
| | Sample_data_processing | Technology: Affymetrix.GeneChip.HG-U133_Plus_2
| | Sample_data_processing | Normalization: Quantile
| | Sample_data_processing | Baseline Transformation: median of all samples
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM978nnn/GSM978175/suppl/GSM978175_MDA-MB-231_control_ETOH_vehicle_.CEL.gz
| | Sample_series_id | GSE39718
| | Sample_series_id | GSE39721
| | Sample_data_row_count | 54675
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GSM978176 | GPL570 |
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MDA-MB-231 Estradiol
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MDA-MB-231 cells, treated for 3 hours with 10-6M estradiol
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cell line: MDA-MB-231
protocol: estradiol treated cells 3h
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| Sample_geo_accession | GSM978176
| | Sample_status | Public on Jul 31 2012
| | Sample_submission_date | Jul 30 2012
| | Sample_last_update_date | Jul 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with Estradiol or ERα17p for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_data_processing | Summarization Algorithm: RMA
| | Sample_data_processing | Technology: Affymetrix.GeneChip.HG-U133_Plus_2
| | Sample_data_processing | Normalization: Quantile
| | Sample_data_processing | Baseline Transformation: median of all samples
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM978nnn/GSM978176/suppl/GSM978176_MDA-MB-231_Estradiol.CEL.gz
| | Sample_series_id | GSE39718
| | Sample_series_id | GSE39721
| | Sample_data_row_count | 54675
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GSM978177 | GPL570 |
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MDA-MB-231 ERα17p
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MDA-MB-231 cells, treated for 3 hours with 10-6M ERα17p
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cell line: MDA-MB-231
protocol: ERα17p treated cells 3h
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| Sample_geo_accession | GSM978177
| | Sample_status | Public on Jul 31 2012
| | Sample_submission_date | Jul 30 2012
| | Sample_last_update_date | Jul 31 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 cells were incubated with Estradiol or ERα17p for 3 hours after preincubation for 4 hours with medium containing 10% charcoal stripped FBS
| | Sample_growth_protocol_ch1 | The human breast cancer cell line MDA-MB-231 was obtained from DSMZ (Braunschweig, Germany) and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, at 37 °C, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA was labeled according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual)
| | Sample_hyb_protocol | RNA was hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), on HGU133A plus 2 chips.
| | Sample_scan_protocol | Signals were detected by an Affymetrix microarray chip reader.
| | Sample_data_processing | Normalization and analysis was performed with the raw data using Genespring GX V11.0 (Agilent, Foster City, CA)
| | Sample_data_processing | Summarization Algorithm: RMA
| | Sample_data_processing | Technology: Affymetrix.GeneChip.HG-U133_Plus_2
| | Sample_data_processing | Normalization: Quantile
| | Sample_data_processing | Baseline Transformation: median of all samples
| | Sample_platform_id | GPL570
| | Sample_contact_name | George,,Notas
| | Sample_contact_email | gnotas@med.uoc.gr
| | Sample_contact_laboratory | Experimental Endocrinology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | University of Crete
| | Sample_contact_address | PO BOX 2208
| | Sample_contact_city | Heraklion
| | Sample_contact_zip/postal_code | 71003
| | Sample_contact_country | Greece
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM978nnn/GSM978177/suppl/GSM978177_MDA-MB-231_ER17p.CEL.gz
| | Sample_series_id | GSE39718
| | Sample_series_id | GSE39721
| | Sample_data_row_count | 54675
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