Search results for the GEO ID: GSE39840  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM980212 | GPL570 |
|
Mono-PBS-1
|
Umbilical Cord blood Monocytes; PBS
|
human umbilical cord blood cells: Monocytes
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980212
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980212/suppl/GSM980212_Mono-PBS-1.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980213 | GPL570 |
|
Mono-PBS-2
|
Umbilical Cord blood Monocytes; PBS
|
human umbilical cord blood cells: Monocytes
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980213
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980213/suppl/GSM980213_Mono-PBS-2.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980214 | GPL570 |
|
Mono-PBS-3
|
Umbilical Cord blood Monocytes; PBS
|
human umbilical cord blood cells: Monocytes
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980214
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980214/suppl/GSM980214_Mono-PBS-3.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980215 | GPL570 |
|
Mono-PBS-4
|
Umbilical Cord blood Monocytes; PBS
|
human umbilical cord blood cells: Monocytes
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980215
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980215/suppl/GSM980215_Mono-PBS-4.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980216 | GPL570 |
|
Mono-PBS-5
|
Umbilical Cord blood Monocytes; PBS
|
human umbilical cord blood cells: Monocytes
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980216
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980216/suppl/GSM980216_Mono-PBS-5.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980217 | GPL570 |
|
Mono-LPS-1
|
Umbilical Cord blood Monocytes; LPS
|
human umbilical cord blood cells: Monocytes
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980217
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980217/suppl/GSM980217_Mono-LPS-1.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980218 | GPL570 |
|
Mono-LPS-2
|
Umbilical Cord blood Monocytes; LPS
|
human umbilical cord blood cells: Monocytes
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980218
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980218/suppl/GSM980218_Mono-LPS-2.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980219 | GPL570 |
|
Mono-LPS-3
|
Umbilical Cord blood Monocytes; LPS
|
human umbilical cord blood cells: Monocytes
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980219
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980219/suppl/GSM980219_Mono-LPS-3.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980220 | GPL570 |
|
Mono-LPS-4
|
Umbilical Cord blood Monocytes; LPS
|
human umbilical cord blood cells: Monocytes
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980220
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980220/suppl/GSM980220_Mono-LPS-4.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980221 | GPL570 |
|
Mono-LPS-5
|
Umbilical Cord blood Monocytes; LPS
|
human umbilical cord blood cells: Monocytes
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Monocytes treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980221
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980221/suppl/GSM980221_Mono-LPS-5.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980222 | GPL570 |
|
PMN-PBS-1
|
Umbilical Cord blood Polymorphonucler cells; PBS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980222
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980222/suppl/GSM980222_PMN-PBS-1.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980223 | GPL570 |
|
PMN-PBS-2
|
Umbilical Cord blood Polymorphonucler cells; PBS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980223
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980223/suppl/GSM980223_PMN-PBS-2.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980224 | GPL570 |
|
PMN-PBS-3
|
Umbilical Cord blood Polymorphonucler cells; PBS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980224
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980224/suppl/GSM980224_PMN-PBS-3.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980225 | GPL570 |
|
PMN-PBS-4
|
Umbilical Cord blood Polymorphonucler cells; PBS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980225
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980225/suppl/GSM980225_PMN-PBS-4.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980226 | GPL570 |
|
PMN-PBS-5
|
Umbilical Cord blood Polymorphonucler cells; PBS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: PBS (vehicle control)
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with 10^ -8 M Dexamethasone for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980226
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980226/suppl/GSM980226_PMN-PBS-5.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980227 | GPL570 |
|
PMN-LPS-1
|
Umbilical Cord blood Polymorphonucler cells; LPS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980227
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980227/suppl/GSM980227_PMN-LPS-1.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980228 | GPL570 |
|
PMN-LPS-2
|
Umbilical Cord blood Polymorphonucler cells; LPS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980228
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980228/suppl/GSM980228_PMN-LPS-2.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980229 | GPL570 |
|
PMN-LPS-3
|
Umbilical Cord blood Polymorphonucler cells; LPS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980229
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980229/suppl/GSM980229_PMN-LPS-3.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980230 | GPL570 |
|
PMN-LPS-4
|
Umbilical Cord blood Polymorphonucler cells; LPS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980230
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980230/suppl/GSM980230_PMN-LPS-4.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
GSM980231 | GPL570 |
|
PMN-LPS-5
|
Umbilical Cord blood Polymorphonucler cells; LPS
|
human umbilical cord blood cells: Polymorphonuclear cells
treatment: LPS (10 ng/mL) for 4 h
|
Gene Expression data from the Umbilical Cord Blood Polymorphonuclear cells treated with PBS vehicle for 1 hr followed by 10ng/ml Lipopolysaccharide stimulation for 4 hrs
|
| Sample_geo_accession | GSM980231
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were pre-incubated with PBS (vehicle control) or stimulated with LPS (10 ng/mL) for 4 h. Cells were harvested and suspended in RNAlater to preserve RNA.
| | Sample_growth_protocol_ch1 | PMNs and MONOs were resuspended in RPMI 1640+10%FCS at 5x106 cells/mL.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was amplified and labled using Ambion MessageAmp II-Biotin enhanced kit. Total RNA and cRNA purity and integrity were monitored by 260/280 and 260/230 ratios respectively, using a Bioanalyzer .
| | Sample_hyb_protocol | Fragmented cRNA (n=5) were hybridized to Affymetrix Human U133 plus 2.0 microarray chips in hybridization oven for 18hr at 45˚C, processed by Gene Chip 450 fluidics station.
| | Sample_scan_protocol | Chips were scanned by Gene Chip 3000 scanner
| | Sample_data_processing | Raw data (n=5 patients) was uploaded onto GeneSifter (internet-based microarray data analysis software), log transformed and normalized using GC-RMA (v 1.1)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dennis,,Davidson
| | Sample_contact_email | dendavidson48@gmail.com
| | Sample_contact_phone | 5164553609
| | Sample_contact_laboratory | Neonatology Research Lab
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | The Feinstein Institute for Medical Research
| | Sample_contact_address | 350 Community Dr
| | Sample_contact_city | Manhasset
| | Sample_contact_state | New York
| | Sample_contact_zip/postal_code | 11030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980231/suppl/GSM980231_PMN-LPS-5.CEL.gz
| | Sample_series_id | GSE39840
| | Sample_data_row_count | 54675
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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