Search results for the GEO ID: GSE39843  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM980285 | GPL570 |
|
CuFi cell line biological rep1
|
human CF cell lines airway
|
treatment: control
cell line: CuFi
genotype: Cystic Fibrosis deltaF508 mutation
|
Gene expression data from CF cell line cultivated on Air-liquid Interface
|
| Sample_geo_accession | GSM980285
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980285/suppl/GSM980285_CuFi_Ctl1.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980286 | GPL570 |
|
CuFi cell line biological rep2
|
human CF cell lines airway
|
treatment: control
cell line: CuFi
genotype: Cystic Fibrosis deltaF508 mutation
|
Gene expression data from CF cell line cultivated on Air-liquid Interface
|
| Sample_geo_accession | GSM980286
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980286/suppl/GSM980286_CuFi_Ctl2.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980287 | GPL570 |
|
CuFi cell line biological rep3
|
human CF cell lines airway
|
treatment: control
cell line: CuFi
genotype: Cystic Fibrosis deltaF508 mutation
|
Gene expression data from CF cell line cultivated on Air-liquid Interface
|
| Sample_geo_accession | GSM980287
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980287/suppl/GSM980287_CuFi_Ctl3.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980288 | GPL570 |
|
CuFi cell line treated at DMNQ biological rep1
|
human CF cell lines airway with 24 hours of DMNQ treatment
|
treatment: DMNQ
cell line: CuFi
genotype: Cystic Fibrosis deltaF508 mutation
|
Gene expression data from CF cell line cultivated on Air-liquid Interface with 24 hours of DMNQ treatment
|
| Sample_geo_accession | GSM980288
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980288/suppl/GSM980288_CuFi_DMNQ1.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980289 | GPL570 |
|
CuFi cell line treated at DMNQ biological rep2
|
human CF cell lines airway with 24 hours of DMNQ treatment
|
treatment: DMNQ
cell line: CuFi
genotype: Cystic Fibrosis deltaF508 mutation
|
Gene expression data from CF cell line cultivated on Air-liquid Interface with 24 hours of DMNQ treatment
|
| Sample_geo_accession | GSM980289
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980289/suppl/GSM980289_CuFi_DMNQ2.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980290 | GPL570 |
|
CuFi cell line treated at DMNQ biological rep3
|
human CF cell lines airway with 24 hours of DMNQ treatment
|
treatment: DMNQ
cell line: CuFi
genotype: Cystic Fibrosis deltaF508 mutation
|
Gene expression data from CF cell line cultivated on Air-liquid Interface with 24 hours of DMNQ treatment
|
| Sample_geo_accession | GSM980290
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980290/suppl/GSM980290_CuFi_DMNQ3.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980291 | GPL570 |
|
NuLi cell line biological rep1
|
human normal cell lines airway
|
treatment: control
cell line: NuLi
genotype: no-Cystic Fibrosis deltaF508 mutation
|
Gene expression data from normal cell line cultivated on Air-liquid Interface
|
| Sample_geo_accession | GSM980291
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980291/suppl/GSM980291_NuLi_Ctl1.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980292 | GPL570 |
|
NuLi cell line biological rep2
|
human normal cell lines airway
|
treatment: control
cell line: NuLi
genotype: no-Cystic Fibrosis deltaF508 mutation
|
Gene expression data from normal CF cell line cultivated on Air-liquid Interface
|
| Sample_geo_accession | GSM980292
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980292/suppl/GSM980292_NuLi_Ctl2.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980293 | GPL570 |
|
NuLi cell line biological rep3
|
human normal cell lines airway
|
treatment: control
cell line: NuLi
genotype: no-Cystic Fibrosis deltaF508 mutation
|
Gene expression data from normalCF cell line cultivated on Air-liquid Interface
|
| Sample_geo_accession | GSM980293
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980293/suppl/GSM980293_NuLi_Ctl3.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980294 | GPL570 |
|
NuLi cell line treated at DMNQ biological rep1
|
human normal cell lines airway with 24 hours of DMNQ treatment
|
treatment: DMNQ
cell line: NuLi
genotype: no-Cystic Fibrosis deltaF508 mutation
|
Gene expression data from normal CF cell line cultivated on Air-liquid Interface with 24 hours of DMNQ treatment
|
| Sample_geo_accession | GSM980294
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980294/suppl/GSM980294_NuLi_DMNQ1.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
| | |
|
GSM980295 | GPL570 |
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NuLi cell line treated at DMNQ biological rep2
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human normal cell lines airway with 24 hours of DMNQ treatment
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treatment: DMNQ
cell line: NuLi
genotype: no-Cystic Fibrosis deltaF508 mutation
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Gene expression data from normal CF cell line cultivated on Air-liquid Interface with 24 hours of DMNQ treatment
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| Sample_geo_accession | GSM980295
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980295/suppl/GSM980295_NuLi_DMNQ2.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
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|
GSM980296 | GPL570 |
|
NuLi cell line treated at DMNQ biological rep3
|
human normal cell lines airway with 24 hours of DMNQ treatment
|
treatment: DMNQ
cell line: NuLi
genotype: no-Cystic Fibrosis deltaF508 mutation
|
Gene expression data from normal cell line cultivated on Air-liquid Interface with 24 hours of DMNQ treatment
|
| Sample_geo_accession | GSM980296
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 02 2012
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation by cell culture at air-liquid interface, the cellular monolayer was treated on apical side with 15 μM DMNQ (2, 3-dimethoxy-1, 4-naphtoquinone) for a 24 hours period
| | Sample_growth_protocol_ch1 | Cultured in an air-liquid condition, cells were coated on collagen plastic dishes with serum-free bronchial epithelial cell growth medium with supplements (BEGM, Cambrex)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAs were purified with the RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The probes were labelled with biotin and fragmented using Affymetrix’s reagents following standard protocol
| | Sample_hyb_protocol | Washing and staining with streptavidin-phycoerythrin (Invitrogen, Carlsbad, CA)
| | Sample_scan_protocol | 10 μg of the resulting cRNA were then loaded on each chip and scanned with a Genechip Scanner 3000 workstation
| | Sample_data_processing | For each hybridized chips, the probe intensity levels were extracted by the Affymetrix® Scanner 3000 7G and the data analyzed by the Microarray Analysis Suite (version 5.0) algorithm. Expression data files were generated for each chip to generate a CEL file
| | Sample_platform_id | GPL570
| | Sample_contact_name | greg,,VOISIN
| | Sample_contact_email | voisingreg@yahoo.fr
| | Sample_contact_laboratory | physiologie pulmonaire
| | Sample_contact_department | Département de Médecine, Centre de Recherche
| | Sample_contact_institute | Centre Hospitalier de l'Université de Montréal Hôtel Dieu
| | Sample_contact_address | 3840 Saint-Urbain
| | Sample_contact_city | Montréal
| | Sample_contact_state | QC
| | Sample_contact_zip/postal_code | H2W 1T6
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980296/suppl/GSM980296_NuLi_DMNQ3.cel.gz
| | Sample_series_id | GSE39843
| | Sample_data_row_count | 54675
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