Search results for the GEO ID: GSE39889  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM980734 | GPL570 |
|
PMN R-Mab, rep1
|
neutrophils, rough Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: rough M. abscessus
treatment: 2 hrs
|
R1
Gene expression data from neutrophils after 2h R-Mab.
|
| Sample_geo_accession | GSM980734
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980734/suppl/GSM980734_R1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980734/suppl/GSM980734_R1.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980735 | GPL570 |
|
PMN Sm-Mab, rep1
|
neutrophils, smooth Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: smooth M. abscessus
treatment: 2 hrs
|
Sm1
Gene expression data from neutrophils after 2h Sm-Mab.
|
| Sample_geo_accession | GSM980735
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980735/suppl/GSM980735_Sm1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980735/suppl/GSM980735_Sm1.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980736 | GPL570 |
|
PMN Sa, rep1
|
neutrophils, S. aureus
|
cell type: blood neutrophils
disease status: healthy
treatment: S. aureus
treatment: 2 hrs
|
Sa1
Gene expression data from neutrophils after 2h Sa.
|
| Sample_geo_accession | GSM980736
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980736/suppl/GSM980736_Sa1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980736/suppl/GSM980736_Sa1.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980738 | GPL570 |
|
PMN R-Mab, rep2
|
neutrophils, rough Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: rough M. abscessus
treatment: 2 hrs
|
R2
Gene expression data from neutrophils after 2h R-Mab.
|
| Sample_geo_accession | GSM980738
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980738/suppl/GSM980738_R2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980738/suppl/GSM980738_R2.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980739 | GPL570 |
|
PMN Sm-Mab, rep2
|
neutrophils, smooth Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: smooth M. abscessus
treatment: 2 hrs
|
Sm2
Gene expression data from neutrophils after 2h Sm-Mab.
|
| Sample_geo_accession | GSM980739
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980739/suppl/GSM980739_Sm2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980739/suppl/GSM980739_Sm2.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980740 | GPL570 |
|
PMN Sa, rep2
|
neutrophils, S. aureus
|
cell type: blood neutrophils
disease status: healthy
treatment: S. aureus
treatment: 2 hrs
|
Sa2
Gene expression data from neutrophils after 2h Sa.
|
| Sample_geo_accession | GSM980740
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980740/suppl/GSM980740_Sa2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980740/suppl/GSM980740_Sa2.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980741 | GPL570 |
|
PMN NS, rep2
|
neutrophils, control
|
cell type: blood neutrophils
disease status: healthy
treatment: saline
treatment: 2 hrs
|
C2
Gene expression data from neutrophils after 2h.
|
| Sample_geo_accession | GSM980741
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980741/suppl/GSM980741_C2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980741/suppl/GSM980741_C2.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980742 | GPL570 |
|
PMN R-Mab, rep3
|
neutrophils, rough Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: rough M. abscessus
treatment: 2 hrs
|
R3
Gene expression data from neutrophils after 2h R-Mab.
|
| Sample_geo_accession | GSM980742
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980742/suppl/GSM980742_R3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980742/suppl/GSM980742_R3.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980743 | GPL570 |
|
PMN Sm-Mab, rep3
|
neutrophils, smooth Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: smooth M. abscessus
treatment: 2 hrs
|
Sm3
Gene expression data from neutrophils after 2h Sm-Mab.
|
| Sample_geo_accession | GSM980743
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980743/suppl/GSM980743_Sm3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980743/suppl/GSM980743_Sm3.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980744 | GPL570 |
|
PMN Sa, rep3
|
neutrophils, S. aureus
|
cell type: blood neutrophils
disease status: healthy
treatment: S. aureus
treatment: 2 hrs
|
Sa3
Gene expression data from neutrophils after 2h Sa.
|
| Sample_geo_accession | GSM980744
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980744/suppl/GSM980744_Sa3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980744/suppl/GSM980744_Sa3.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980745 | GPL570 |
|
PMN NS, rep3
|
neutrophils, control
|
cell type: blood neutrophils
disease status: healthy
treatment: saline
treatment: 2 hrs
|
C3
Gene expression data from neutrophils after 2h.
|
| Sample_geo_accession | GSM980745
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980745/suppl/GSM980745_C3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980745/suppl/GSM980745_C3.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980746 | GPL570 |
|
PMN R-Mab, rep4
|
neutrophils, rough Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: rough M. abscessus
treatment: 2 hrs
|
R4
Gene expression data from neutrophils after 2h R-Mab.
|
| Sample_geo_accession | GSM980746
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980746/suppl/GSM980746_R4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980746/suppl/GSM980746_R4.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980747 | GPL570 |
|
PMN Sm-Mab, rep4
|
neutrophils, smooth Mab
|
cell type: blood neutrophils
disease status: healthy
treatment: smooth M. abscessus
treatment: 2 hrs
|
Sm4
Gene expression data from neutrophils after 2h Sm-Mab.
|
| Sample_geo_accession | GSM980747
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980747/suppl/GSM980747_Sm4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980747/suppl/GSM980747_Sm4.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980748 | GPL570 |
|
PMN Sa, rep4
|
neutrophils, S. aureus
|
cell type: blood neutrophils
disease status: healthy
treatment: S. aureus
treatment: 2 hrs
|
Sa4
Gene expression data from neutrophils after 2h Sa.
|
| Sample_geo_accession | GSM980748
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980748/suppl/GSM980748_Sa4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980748/suppl/GSM980748_Sa4.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
|
GSM980749 | GPL570 |
|
PMN NS, rep4
|
neutrophils, control
|
cell type: blood neutrophils
disease status: healthy
treatment: saline
treatment: 2 hrs
|
C4
Gene expression data from neutrophils after 2h.
|
| Sample_geo_accession | GSM980749
| | Sample_status | Public on Jan 10 2013
| | Sample_submission_date | Aug 05 2012
| | Sample_last_update_date | Aug 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
| | Sample_growth_protocol_ch1 | Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Trizol Reagent (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
| | Sample_hyb_protocol | Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix)
| | Sample_data_processing | The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ken,,Malcolm
| | Sample_contact_email | malcolmk@njhealth.org
| | Sample_contact_institute | National Jewish Health
| | Sample_contact_address | 1400 Jackson St
| | Sample_contact_city | Denver
| | Sample_contact_zip/postal_code | 80206
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980749/suppl/GSM980749_C4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM980nnn/GSM980749/suppl/GSM980749_C4.CHP.gz
| | Sample_series_id | GSE39889
| | Sample_data_row_count | 54675
| | |
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