Search results for the GEO ID: GSE39979 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM982854 | GPL1355 |
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Cultured astrocyte control
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Rat embryo hippocampus
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cell type: astrocyte
genotype: normal
age: embryonic day 18
transfection: vector control
genetic background: Sprague-Dawley
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Gene expression data from astrocytes expressing IRES2-RFP, i.e. control
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Sample_geo_accession | GSM982854
| Sample_status | Public on Aug 09 2012
| Sample_submission_date | Aug 08 2012
| Sample_last_update_date | Aug 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Cultured astrocytes were washed with PBS, and RNeasy (Qiagen) was used.
| Sample_growth_protocol_ch1 | Kept at 37C and 5% CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy was used according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat expression 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kazunori,,Kanemaru
| Sample_contact_laboratory | Masamitsu Iino Lab
| Sample_contact_department | Department of Pharmacology
| Sample_contact_institute | Graduate School of Medicine, The University of Tokyo
| Sample_contact_address | 7-3-1 Hongo, Bunkyo-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 113-0033
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM982nnn/GSM982854/suppl/GSM982854_ID.CEL.gz
| Sample_series_id | GSE39979
| Sample_data_row_count | 31099
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GSM982855 | GPL1355 |
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Cultured astrocyte 5ppase
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Rat embryo hippocampus
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cell type: astrocyte
genotype: normal
age: embryonic day 18
transfection: 55ppase-IRES-RFP
genetic background: Sprague-Dawley
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Gene expression data from astrocytes expressing 5ppase-IRES2-RFP
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Sample_geo_accession | GSM982855
| Sample_status | Public on Aug 09 2012
| Sample_submission_date | Aug 08 2012
| Sample_last_update_date | Aug 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Cultured astrocytes were washed with PBS, and RNeasy (Qiagen) was used.
| Sample_growth_protocol_ch1 | Kept at 37C and 5% CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy was used according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Rat expression 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Kazunori,,Kanemaru
| Sample_contact_laboratory | Masamitsu Iino Lab
| Sample_contact_department | Department of Pharmacology
| Sample_contact_institute | Graduate School of Medicine, The University of Tokyo
| Sample_contact_address | 7-3-1 Hongo, Bunkyo-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 113-0033
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM982nnn/GSM982855/suppl/GSM982855_5P.CEL.gz
| Sample_series_id | GSE39979
| Sample_data_row_count | 31099
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