Result

Search results for the GEO ID: GSE39999

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM983106

GPL570

Human iDC stimulated with IL-8, replicate 1

Human iDC_IL-8

tissue source: peripheral blood mononuclear cells responder cells: immature dendritic cells (iDCs) stimulated with: 1 mg/l human recombinant IL-8

IL8 KRON15

Sample_geo_accession	GSM983106
Sample_status	Public on Dec 08 2012
Sample_submission_date	Aug 08 2012
Sample_last_update_date	Dec 08 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	On day 7 iDCs were either left without stimulation (2 control samples) or stimulated with 1 mg/l human recombinant IL-8 (2 IL-8 samples) or with recombinant Brugia malayi AsnRS (2 samples rBmAsnRS). After 48 hours, cells were harvested, pelleted and frozen at -86*C (in TRIzol) for RNA extraction.
Sample_growth_protocol_ch1	Human immature dendritic cells (iDCs) were generated from CD14+ cells isolated from human peripheral blood mononuclear cells, cultured in 6 well plates for 5 days with 50 ng/ml of IL-4 and GM-CSF (1000 IU/ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
Sample_hyb_protocol	The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
Sample_scan_protocol	After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
Sample_data_processing	Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) to determine signal log ratios. The mean fold change was calculated from the 2 independent biological replicates for each of the 3 experimental conditions (6 arrays total). The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in BioConductor (RankProd, www.bioconductor.org/).
Sample_platform_id	GPL570
Sample_contact_name	Michael,Andrew,Kron
Sample_contact_email	mkron@mcw.edu
Sample_contact_phone	414 955 5613
Sample_contact_fax	414 955 6568
Sample_contact_laboratory	BIotechnology and Bioengineering
Sample_contact_department	Medicine
Sample_contact_institute	Medical College of Wisconsin
Sample_contact_address	8701 Watertown Plank Road
Sample_contact_city	Milwaukee
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53206
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM983nnn/GSM983106/suppl/GSM983106_15_KRON_2_IL-8_4-13-11.CEL.gz
Sample_series_id	GSE39999
Sample_data_row_count	54675

GSM983107

GPL570

Human iDC stimulated with IL-8, replicate 2

Human iDC_IL-8

tissue source: peripheral blood mononuclear cells responder cells: immature dendritic cells (iDCs) stimulated with: 1 mg/l human recombinant IL-8

IL8 KRON7124

Sample_geo_accession	GSM983107
Sample_status	Public on Dec 08 2012
Sample_submission_date	Aug 08 2012
Sample_last_update_date	Dec 08 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	On day 7 iDCs were either left without stimulation (2 control samples) or stimulated with 1 mg/l human recombinant IL-8 (2 IL-8 samples) or with recombinant Brugia malayi AsnRS (2 samples rBmAsnRS). After 48 hours, cells were harvested, pelleted and frozen at -86*C (in TRIzol) for RNA extraction.
Sample_growth_protocol_ch1	Human immature dendritic cells (iDCs) were generated from CD14+ cells isolated from human peripheral blood mononuclear cells, cultured in 6 well plates for 5 days with 50 ng/ml of IL-4 and GM-CSF (1000 IU/ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
Sample_hyb_protocol	The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
Sample_scan_protocol	After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
Sample_data_processing	Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) to determine signal log ratios. The mean fold change was calculated from the 2 independent biological replicates for each of the 3 experimental conditions (6 arrays total). The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in BioConductor (RankProd, www.bioconductor.org/).
Sample_platform_id	GPL570
Sample_contact_name	Michael,Andrew,Kron
Sample_contact_email	mkron@mcw.edu
Sample_contact_phone	414 955 5613
Sample_contact_fax	414 955 6568
Sample_contact_laboratory	BIotechnology and Bioengineering
Sample_contact_department	Medicine
Sample_contact_institute	Medical College of Wisconsin
Sample_contact_address	8701 Watertown Plank Road
Sample_contact_city	Milwaukee
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53206
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM983nnn/GSM983107/suppl/GSM983107_KRON_IL-8_52001900911908020113412666987124.CEL.gz
Sample_series_id	GSE39999
Sample_data_row_count	54675

GSM983108

GPL570

Human iDC stimulated with AsnRS, replicate 1

Human iDC_AsnRS

tissue source: peripheral blood mononuclear cells responder cells: immature dendritic cells (iDCs) stimulated with: recombinant Brugia malayi AsnRS

AsnRS KRON16

Sample_geo_accession	GSM983108
Sample_status	Public on Dec 08 2012
Sample_submission_date	Aug 08 2012
Sample_last_update_date	Dec 08 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	On day 7 iDCs were either left without stimulation (2 control samples) or stimulated with 1 mg/l human recombinant IL-8 (2 IL-8 samples) or with recombinant Brugia malayi AsnRS (2 samples rBmAsnRS). After 48 hours, cells were harvested, pelleted and frozen at -86*C (in TRIzol) for RNA extraction.
Sample_growth_protocol_ch1	Human immature dendritic cells (iDCs) were generated from CD14+ cells isolated from human peripheral blood mononuclear cells, cultured in 6 well plates for 5 days with 50 ng/ml of IL-4 and GM-CSF (1000 IU/ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
Sample_hyb_protocol	The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
Sample_scan_protocol	After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
Sample_data_processing	Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) to determine signal log ratios. The mean fold change was calculated from the 2 independent biological replicates for each of the 3 experimental conditions (6 arrays total). The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in BioConductor (RankProd, www.bioconductor.org/).
Sample_platform_id	GPL570
Sample_contact_name	Michael,Andrew,Kron
Sample_contact_email	mkron@mcw.edu
Sample_contact_phone	414 955 5613
Sample_contact_fax	414 955 6568
Sample_contact_laboratory	BIotechnology and Bioengineering
Sample_contact_department	Medicine
Sample_contact_institute	Medical College of Wisconsin
Sample_contact_address	8701 Watertown Plank Road
Sample_contact_city	Milwaukee
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53206
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM983nnn/GSM983108/suppl/GSM983108_16_KRON_3_AsnRS_4-13-11.CEL.gz
Sample_series_id	GSE39999
Sample_data_row_count	54675

GSM983109

GPL570

Human iDC stimulated with AsnRS, replicate 2

Human iDC_AsnRS

tissue source: peripheral blood mononuclear cells responder cells: immature dendritic cells (iDCs) stimulated with: recombinant Brugia malayi AsnRS

AsnRS KRON7139

Sample_geo_accession	GSM983109
Sample_status	Public on Dec 08 2012
Sample_submission_date	Aug 08 2012
Sample_last_update_date	Dec 08 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	On day 7 iDCs were either left without stimulation (2 control samples) or stimulated with 1 mg/l human recombinant IL-8 (2 IL-8 samples) or with recombinant Brugia malayi AsnRS (2 samples rBmAsnRS). After 48 hours, cells were harvested, pelleted and frozen at -86*C (in TRIzol) for RNA extraction.
Sample_growth_protocol_ch1	Human immature dendritic cells (iDCs) were generated from CD14+ cells isolated from human peripheral blood mononuclear cells, cultured in 6 well plates for 5 days with 50 ng/ml of IL-4 and GM-CSF (1000 IU/ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
Sample_hyb_protocol	The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
Sample_scan_protocol	After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
Sample_data_processing	Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) to determine signal log ratios. The mean fold change was calculated from the 2 independent biological replicates for each of the 3 experimental conditions (6 arrays total). The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in BioConductor (RankProd, www.bioconductor.org/).
Sample_platform_id	GPL570
Sample_contact_name	Michael,Andrew,Kron
Sample_contact_email	mkron@mcw.edu
Sample_contact_phone	414 955 5613
Sample_contact_fax	414 955 6568
Sample_contact_laboratory	BIotechnology and Bioengineering
Sample_contact_department	Medicine
Sample_contact_institute	Medical College of Wisconsin
Sample_contact_address	8701 Watertown Plank Road
Sample_contact_city	Milwaukee
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53206
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM983nnn/GSM983109/suppl/GSM983109_KRON_AsnRS_52001900911908020113412666987139.CEL.gz
Sample_series_id	GSE39999
Sample_data_row_count	54675

GSM983110

GPL570

Human iDC control no stimulation, replicate 1

Human iDC control_no stimulation

tissue source: peripheral blood mononuclear cells responder cells: immature dendritic cells (iDCs)

Ctrl KRON14

Sample_geo_accession	GSM983110
Sample_status	Public on Dec 08 2012
Sample_submission_date	Aug 08 2012
Sample_last_update_date	Dec 08 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	On day 7 iDCs were either left without stimulation (2 control samples) or stimulated with 1 mg/l human recombinant IL-8 (2 IL-8 samples) or with recombinant Brugia malayi AsnRS (2 samples rBmAsnRS). After 48 hours, cells were harvested, pelleted and frozen at -86*C (in TRIzol) for RNA extraction.
Sample_growth_protocol_ch1	Human immature dendritic cells (iDCs) were generated from CD14+ cells isolated from human peripheral blood mononuclear cells, cultured in 6 well plates for 5 days with 50 ng/ml of IL-4 and GM-CSF (1000 IU/ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
Sample_hyb_protocol	The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
Sample_scan_protocol	After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
Sample_data_processing	Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) to determine signal log ratios. The mean fold change was calculated from the 2 independent biological replicates for each of the 3 experimental conditions (6 arrays total). The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in BioConductor (RankProd, www.bioconductor.org/).
Sample_platform_id	GPL570
Sample_contact_name	Michael,Andrew,Kron
Sample_contact_email	mkron@mcw.edu
Sample_contact_phone	414 955 5613
Sample_contact_fax	414 955 6568
Sample_contact_laboratory	BIotechnology and Bioengineering
Sample_contact_department	Medicine
Sample_contact_institute	Medical College of Wisconsin
Sample_contact_address	8701 Watertown Plank Road
Sample_contact_city	Milwaukee
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53206
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM983nnn/GSM983110/suppl/GSM983110_14_KRON_1_control_4-13-11.CEL.gz
Sample_series_id	GSE39999
Sample_data_row_count	54675

GSM983111

GPL570

Human iDC control no stimulation, replicate 2

Human iDC control_no stimulation

tissue source: peripheral blood mononuclear cells responder cells: immature dendritic cells (iDCs)

Ctrl KRON1862

Sample_geo_accession	GSM983111
Sample_status	Public on Dec 08 2012
Sample_submission_date	Aug 08 2012
Sample_last_update_date	Dec 08 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	On day 7 iDCs were either left without stimulation (2 control samples) or stimulated with 1 mg/l human recombinant IL-8 (2 IL-8 samples) or with recombinant Brugia malayi AsnRS (2 samples rBmAsnRS). After 48 hours, cells were harvested, pelleted and frozen at -86*C (in TRIzol) for RNA extraction.
Sample_growth_protocol_ch1	Human immature dendritic cells (iDCs) were generated from CD14+ cells isolated from human peripheral blood mononuclear cells, cultured in 6 well plates for 5 days with 50 ng/ml of IL-4 and GM-CSF (1000 IU/ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and amplified using an Affymetrix two-cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cRNA was synthesized, labeled, fragmented, and hybridized to an array in accordance to the Affymetrix GeneChip expression analysis technical manual.
Sample_hyb_protocol	The GeneChip human genome U133 plus 2.0 array was selected for these studies for its overall comprehensive coverage.
Sample_scan_protocol	After hybridization, arrays were washed and stained with Affymetrix fluidics protocol FS450_0001 and scanned with a 7G Affymetrix GeneChip Scanner
Sample_data_processing	Image data were analyzed with Affymetrix Expression Console™ software and normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) to determine signal log ratios. The mean fold change was calculated from the 2 independent biological replicates for each of the 3 experimental conditions (6 arrays total). The statistical significance of differential gene expression and false discovery rates (FDR) were derived through a Rank Product algorithm available as an analysis package in BioConductor (RankProd, www.bioconductor.org/).
Sample_platform_id	GPL570
Sample_contact_name	Michael,Andrew,Kron
Sample_contact_email	mkron@mcw.edu
Sample_contact_phone	414 955 5613
Sample_contact_fax	414 955 6568
Sample_contact_laboratory	BIotechnology and Bioengineering
Sample_contact_department	Medicine
Sample_contact_institute	Medical College of Wisconsin
Sample_contact_address	8701 Watertown Plank Road
Sample_contact_city	Milwaukee
Sample_contact_state	Wisconsin
Sample_contact_zip/postal_code	53206
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM983nnn/GSM983111/suppl/GSM983111_KRON_control_52001900909212101112411652471862.CEL.gz
Sample_series_id	GSE39999
Sample_data_row_count	54675

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