Search results for the GEO ID: GSE40051  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM984404 | GPL570 |
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None target siRNA
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U87 glioma cell transfected with none target siRNA
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cell line: U87
cell type: glioblastoma cells
sirna: control
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| Sample_geo_accession | GSM984404
| | Sample_status | Public on Aug 11 2012
| | Sample_submission_date | Aug 10 2012
| | Sample_last_update_date | Aug 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | According to the Lipofectamin 2000 (Invitogen).
| | Sample_growth_protocol_ch1 | 37 degrees with 5% CO2 humid cuture, 10% FBS in DMEM medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRIZOL Reagent (Cat#15596-018, Life Technologies, Carlsbad, CA, US) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using the GeneChip 3'IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer's instructions to obtain biotin-labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash were performed using the GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US) in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US) and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) following the manufacturer's instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by the MAS 5.0 algorithm, GeneSpring Software 11.0 (Agilent Technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL570
| | Sample_contact_name | nu,,zhang
| | Sample_contact_email | zhangxiaonu@gmail.com
| | Sample_contact_department | neurosurgery
| | Sample_contact_institute | 1st affiliated hospital of Sun Yat-sen university
| | Sample_contact_address | no.58 Zhongshan 2 road
| | Sample_contact_city | guangzhou
| | Sample_contact_zip/postal_code | 510080
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM984nnn/GSM984404/suppl/GSM984404_BH12333-2_NC_HG-U133_Plus_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM984nnn/GSM984404/suppl/GSM984404_BH12333-2_NC_HG-U133_Plus_2.mas5.CHP.gz
| | Sample_series_id | GSE40051
| | Sample_data_row_count | 54675
| | |
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GSM984405 | GPL570 |
|
FOXM1 siRNA
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U87 glioma cell transfected with FOXM1 siRNA
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cell line: U87
cell type: glioblastoma cells
sirna: FOXM1
|
|
| Sample_geo_accession | GSM984405
| | Sample_status | Public on Aug 11 2012
| | Sample_submission_date | Aug 10 2012
| | Sample_last_update_date | Aug 11 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | According to the Lipofectamin 2000 (Invitogen).
| | Sample_growth_protocol_ch1 | 37 degrees with 5% CO2 humid cuture, 10% FBS in DMEM medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using TRIZOL Reagent (Cat#15596-018, Life Technologies, Carlsbad, CA, US) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA were amplified, labeled and purified by using the GeneChip 3'IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer's instructions to obtain biotin-labeled cRNA.
| | Sample_hyb_protocol | Array hybridization and wash were performed using the GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US) in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US) and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) following the manufacturer's instructions.
| | Sample_scan_protocol | Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
| | Sample_data_processing | Raw data were normalized by the MAS 5.0 algorithm, GeneSpring Software 11.0 (Agilent Technologies, Santa Clara, CA, US).
| | Sample_platform_id | GPL570
| | Sample_contact_name | nu,,zhang
| | Sample_contact_email | zhangxiaonu@gmail.com
| | Sample_contact_department | neurosurgery
| | Sample_contact_institute | 1st affiliated hospital of Sun Yat-sen university
| | Sample_contact_address | no.58 Zhongshan 2 road
| | Sample_contact_city | guangzhou
| | Sample_contact_zip/postal_code | 510080
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM984nnn/GSM984405/suppl/GSM984405_BH12333-2_SI_HG-U133_Plus_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM984nnn/GSM984405/suppl/GSM984405_BH12333-2_SI_HG-U133_Plus_2.mas5.CHP.gz
| | Sample_series_id | GSE40051
| | Sample_data_row_count | 54675
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