Search results for the GEO ID: GSE40286  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM990680 | GPL1261 |
|
MEF, WT_serum_0min
|
MEF cell, control, no serum
|
genotype/variation: wild type
cell type: embryonic fibroblast
developmental stage: 9.5-day embryos
treatment: no serum
|
Gene expression data from WT MEF
|
| Sample_geo_accession | GSM990680
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990680/suppl/GSM990680_WT-1.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990681 | GPL1261 |
|
MEF, WT_serum_30min
|
MEF cell, control, 30min serum
|
genotype/variation: wild type
cell type: embryonic fibroblast
developmental stage: 9.5-day embryos
treatment: serum for 30min
|
Gene expression data from WT MEF cultured with serum for 30min
|
| Sample_geo_accession | GSM990681
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990681/suppl/GSM990681_WT-2.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990682 | GPL1261 |
|
MEF, WT_serum_90min
|
MEF cell, control, 90min serum
|
genotype/variation: wild type
cell type: embryonic fibroblast
developmental stage: 9.5-day embryos
treatment: serum for 90min
|
Gene expression data from WT MEF cultured with serum for 90min
|
| Sample_geo_accession | GSM990682
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990682/suppl/GSM990682_WT-3.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990683 | GPL1261 |
|
MEF, Med23KO_serum_0min
|
MEF cell, Med23 deficiency , no serum
|
genotype/variation: Med23KO
cell type: embryonic fibroblast
developmental stage: 9.5-day embryos
treatment: no serum
|
Gene expression data from Med23 KO MEF
|
| Sample_geo_accession | GSM990683
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990683/suppl/GSM990683_KO-1.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990684 | GPL1261 |
|
MEF, Med23KO_serum_30min
|
MEF cell, Med23 deficiency , 30min serum
|
genotype/variation: Med23KO
cell type: embryonic fibroblast
developmental stage: 9.5-day embryos
treatment: serum for 30min
|
Gene expression data from Med23 KO cultured with serum for 30min
|
| Sample_geo_accession | GSM990684
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990684/suppl/GSM990684_KO-2.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990685 | GPL1261 |
|
MEF, Med23KO_serum_90min
|
MEF cell, Med23 deficiency , 90min serum
|
genotype/variation: Med23KO
cell type: embryonic fibroblast
developmental stage: 9.5-day embryos
treatment: serum for 90min
|
Gene expression data from Med23 KO cultured with serum for 90min
|
| Sample_geo_accession | GSM990685
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990685/suppl/GSM990685_KO-3.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990686 | GPL1261 |
|
10T1/2, control
|
10T1/2 cell, control
|
cell type: embryonic fibroblast
developmental stage: cell line derived from embryo
treatment: none
|
Gene expression data from 10T1/2 control
|
| Sample_geo_accession | GSM990686
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990686/suppl/GSM990686_A2-YJW.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990687 | GPL1261 |
|
10T1/2, MAL_overexpression
|
10T1/2 cell, MAL overexpression
|
cell type: embryonic fibroblast
developmental stage: cell line derived from embryo
treatment: MAL overexpression
|
Gene expression data from 10T1/2 with MAL overexpression
|
| Sample_geo_accession | GSM990687
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990687/suppl/GSM990687_B2-YJW.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990688 | GPL1261 |
|
10T1/2, Med23_knockdown
|
10T1/2 cell, Med23 deficiency
|
cell type: embryonic fibroblast
developmental stage: cell line derived from embryo
treatment: MED23 deficiency
|
Gene expression data from 10T1/2 with MED23 deficiency
|
| Sample_geo_accession | GSM990688
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990688/suppl/GSM990688_C2-YJW.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
|
GSM990689 | GPL1261 |
|
10T1/2, both_treatment
|
10T1/2 cell, both MAL overexpression and Med23 deficiency
|
cell type: embryonic fibroblast
developmental stage: cell line derived from embryo
treatment: MAL overexpression and MED23 deficiency
|
Gene expression data from 10T1/2 with both MAL overexpression and MED23 deficiency
|
| Sample_geo_accession | GSM990689
| | Sample_status | Public on Oct 01 2012
| | Sample_submission_date | Aug 22 2012
| | Sample_last_update_date | Oct 01 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatment prior to experiment was applied.
| | Sample_growth_protocol_ch1 | WT and Med23 KO MEFs were isolated from 9.5-day embryos and self-immortalized from standard protocol (Balamotis et al. 2009; Wang et al. 2009). MEF and 10T1/2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS (Hyclone) in 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The Affymetrix GeneChip arrays were hybridized and washed using the manufacturer's protocol.
| | Sample_scan_protocol | Affymetrix GeneChip arrays were scanned using the Affymetrix GeneArray scanner.
| | Sample_data_processing | The microarray data were normalized using the Robust Multichip Average (RMA) method. We discarded probe sets that did not have signals above background in more than 75% of the arrays for all samples using the Affymetrix MAS5 present/absent calls.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiyeon,,Park
| | Sample_contact_email | parkji@umdnj.edu
| | Sample_contact_department | Cell Biology and Molecular Medicine
| | Sample_contact_institute | UMDNJ
| | Sample_contact_address | 185 South Orange Ave
| | Sample_contact_city | Newark
| | Sample_contact_state | NJ
| | Sample_contact_zip/postal_code | 07101-1709
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM990nnn/GSM990689/suppl/GSM990689_D2-YJW.CEL.gz
| | Sample_series_id | GSE40286
| | Sample_data_row_count | 45101
| | |
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